In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia

Nur Azna Saari, Nor Hazwani Hamzah, Mohamed Tajuddin Abdul Manaf, Lee Kok Leong, Alia Syafiqah Aznan, Wan Nurhafizah Wan Ibrahim, Nurul Aqilah Iberahim, Khairulbariyyah Zakaria, Nadirah Musa, Laith Abdul Razzak, Muhd Danish Daniel Abdullah, Shumpei Ieahata, Sandra Catherine Zainathan, Sharifah Noor Emilia Syed Jamil Fadaak, Sharifah Rahmah Syed Muhammad, Yeong Yik Sung, Mazlan Abd. Ghaffar, Najiah Musa

Research output: Contribution to journalArticle

Abstract

Mud crabs are among the commercially important aquatic animals because of high demand in Asian countries. Currently, one of the main constraints for expansion of mud crab aquaculture is the inconsistent and low hatchery production of crab juveniles. The use of beneficial bacteria offers a promising approach in aquaculture to prevent infections by pathogenic bacteria, and has been shown to help reduce mortality after infection. The goals of this study were to isolate, identify and evaluate potential probiotic bacteria from the gut of wild orange mud crab from Setiu Wetlands, Terengganu. Isolate was identified using BBLTM Crystal Gram Positive (GP) Identification System. Probiotic properties were evaluated including antibiotic susceptibility, tolerances to pH, bile salt, trypsin and pepsin, autoaggregation and coaggregation with Vibrio pathogens. Isolate UMTK003 was identified as Bacillus licheniformis. The isolate was able to withstand pH 4.5 to 9.5. Bile salt, pepsin and trypsin tolerances were 0.1-0.4%, 0.1-0.7% and 0.4-1%, respectively. Antibiotic susceptibility profiling showed multiple antibiotic resistance (MAR) index of 0.15. Autoaggregation was 79.83 ± 0.0015%. Coaggregation varied from 57.71 ± 0.0050% (Vibrio harveyi), 52.41 ± 0.0011% (Vibrio metschnikovii), 50.07 ± 0.0011% (Vibrio cholera) to 43.25 ± 0.0010% (Vibrio parahaemolyticus). These results suggested probiotic potential of B. licheniformis strain UMTK003 for mud crab aquaculture in Malaysia.

Original languageEnglish
Pages (from-to)62-74
Number of pages13
JournalJournal of Sustainability Science and Management
Volume2017
Issue numberSpecial Issue 3
Publication statusPublished - 1 Jan 2017

Fingerprint

Aquaculture
probiotics
Wetlands
Antibiotics
Bacilli
wetland
tolerance
Malaysia
crab
Bacteria
mud
aquaculture
evaluation
Salts
mortality
animal
antibiotics
bacterium
Pathogens
demand

Keywords

  • Mud crab
  • Probiotic
  • Scylla olivacea
  • Setiu Wetlands

ASJC Scopus subject areas

  • Geography, Planning and Development
  • Pollution
  • Management, Monitoring, Policy and Law

Cite this

In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia. / Saari, Nur Azna; Hamzah, Nor Hazwani; Manaf, Mohamed Tajuddin Abdul; Leong, Lee Kok; Aznan, Alia Syafiqah; Ibrahim, Wan Nurhafizah Wan; Iberahim, Nurul Aqilah; Zakaria, Khairulbariyyah; Musa, Nadirah; Razzak, Laith Abdul; Abdullah, Muhd Danish Daniel; Ieahata, Shumpei; Zainathan, Sandra Catherine; Fadaak, Sharifah Noor Emilia Syed Jamil; Muhammad, Sharifah Rahmah Syed; Sung, Yeong Yik; Abd. Ghaffar, Mazlan; Musa, Najiah.

In: Journal of Sustainability Science and Management, Vol. 2017, No. Special Issue 3, 01.01.2017, p. 62-74.

Research output: Contribution to journalArticle

Saari, NA, Hamzah, NH, Manaf, MTA, Leong, LK, Aznan, AS, Ibrahim, WNW, Iberahim, NA, Zakaria, K, Musa, N, Razzak, LA, Abdullah, MDD, Ieahata, S, Zainathan, SC, Fadaak, SNESJ, Muhammad, SRS, Sung, YY, Abd. Ghaffar, M & Musa, N 2017, 'In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia', Journal of Sustainability Science and Management, vol. 2017, no. Special Issue 3, pp. 62-74.
Saari, Nur Azna ; Hamzah, Nor Hazwani ; Manaf, Mohamed Tajuddin Abdul ; Leong, Lee Kok ; Aznan, Alia Syafiqah ; Ibrahim, Wan Nurhafizah Wan ; Iberahim, Nurul Aqilah ; Zakaria, Khairulbariyyah ; Musa, Nadirah ; Razzak, Laith Abdul ; Abdullah, Muhd Danish Daniel ; Ieahata, Shumpei ; Zainathan, Sandra Catherine ; Fadaak, Sharifah Noor Emilia Syed Jamil ; Muhammad, Sharifah Rahmah Syed ; Sung, Yeong Yik ; Abd. Ghaffar, Mazlan ; Musa, Najiah. / In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia. In: Journal of Sustainability Science and Management. 2017 ; Vol. 2017, No. Special Issue 3. pp. 62-74.
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