In Vitro Evaluation of Human Hand Tendon Ingrowth into A Synthetic Scaffold

Shalimar Abdullah, Fadzlina Mohtar, Nordashima Abdul Shukor, Jamari Sapuan

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Synthetic scaffold has been used for tissue approximation and reconstructing damaged and torn ligaments. This study explores the ability of tendon ingrowth into a synthetic scaffold in vitro, evaluate growth characteristics, morphology and deposition of collagen matrix into a synthetic scaffold. METHODS: Upper limb tendons were harvested with consent from patients with crush injuries and non-replantable amputations. These tendons (both extensor and flexor) measuring 1 cm are sutured to either side of a 0.5 cm synthetic tendon strip and cultured in growth medium. At 2, 4, 6 and 8 weeks, samples were fixed into paraffin blocks, cut and stained with haematoxylin-eosin (H&E) and Masson's trichrome. RESULTS: Minimal tendon ingrowth were seen in the first 2 weeks of incubation. However at 4 weeks, the cell ingrowth were seen migrating towards the junction between the tendon and the synthetic scaffold. This ingrowth continued to expand at 6 weeks and up to 8 weeks. At this point, the demarcation between human tendon and synthetic scaffold was indistinct. CONCLUSIONS: We conclude that tendon ingrowth composed of collagen matrix were able to proliferate into a synthetic scaffold in vitro.

Original languageEnglish
Pages (from-to)429-434
Number of pages6
JournalThe journal of hand surgery Asian-Pacific volume
Volume22
Issue number4
DOIs
Publication statusPublished - 1 Dec 2017

Fingerprint

Tendons
Hand
Collagen
In Vitro Techniques
Hematoxylin
Eosine Yellowish-(YS)
Growth
Amputation
Ligaments
Upper Extremity
Paraffin

Keywords

  • Extensor tendon
  • Flexor tendon
  • Synthetic scaffold
  • Tendon graft
  • Tendon ingrowth

ASJC Scopus subject areas

  • Medicine(all)

Cite this

In Vitro Evaluation of Human Hand Tendon Ingrowth into A Synthetic Scaffold. / Abdullah, Shalimar; Mohtar, Fadzlina; Abdul Shukor, Nordashima; Sapuan, Jamari.

In: The journal of hand surgery Asian-Pacific volume, Vol. 22, No. 4, 01.12.2017, p. 429-434.

Research output: Contribution to journalArticle

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