In-vitro differentiation study on isolated human mesenchymal stem cells.

P. L. Mok, S. K. Cheong, Leong Chooi Fun

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Mesenchymal stem cells are pluripotent progenitors that could be found in human bone marrow. Mesenchymal stem cells are capable of renewing themselves without differentiation in long-term culture. These cells also have low immunogenicity and can suppress alloreactive T cell responses. In the current study, mesenchymal stem cells isolated and propagated previously from the bone marrow of a megaloblastic anaemia patient were tested for their capabilities to differentiate into adipocytes, chondrocytes and osteoblasts in vitro. The differentiated cells were determined by Oil Red O, Alcian Blue-PAS and Alizarin Red S staining, and reverse transcriptase-polymerase chain reaction to determine the expression of mRNA specific for adipogenesis, chondrogenesis and osteogenesis. The results showed that the fibroblast-like cells were capable of differentiating into adipocytes, chondrocytes and osteoblasts upon chemical induction. The adipocytes, chondrocytes and osteoblasts were stained positively to Oil Red O, Alcian Blue-PAS and Alizarin Red S respectively. The differentiated cells were also found to express mRNA specific for adipogenesis ('peroxisome proliferation-activated receptor gamma2' and lipoprotein lipase), chondrogenesis (collagen type II) and osteogenesis (osteocalcin, osteopontin and alkaline phosphatase). In conclusion, this research has successfully isolated fibroblast-like cells from human bone marrow and these cells demonstrated morphological, cytochemical and immunochemical characteristics similar to mesenchymal stem cells. These cells maintain their proliferative properties and could be differentiated into the mesoderm lineage. The success of this study is vital because mesenchymal stem cells can be used in cellular therapy to regenerate or replace damaged tissues, or as a vehicle for therapeutic gene delivery in the future.

Original languageEnglish
Pages (from-to)11-19
Number of pages9
JournalThe Malaysian journal of pathology
Volume30
Issue number1
Publication statusPublished - Jun 2008

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Mesenchymal Stromal Cells
Chondrocytes
Osteoblasts
Adipocytes
Chondrogenesis
Adipogenesis
Alcian Blue
Osteogenesis
Fibroblasts
Bone Marrow
Megaloblastic Anemia
Messenger RNA
Osteopontin
Peroxisomes
Collagen Type II
Lipoprotein Lipase
Osteocalcin
Mesoderm
Reverse Transcriptase Polymerase Chain Reaction
Bone Marrow Cells

ASJC Scopus subject areas

  • Medicine(all)

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In-vitro differentiation study on isolated human mesenchymal stem cells. / Mok, P. L.; Cheong, S. K.; Chooi Fun, Leong.

In: The Malaysian journal of pathology, Vol. 30, No. 1, 06.2008, p. 11-19.

Research output: Contribution to journalArticle

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