In vitro cytotoxicity effects of single and combination Nigella sativa and Zingiber zerumbet extracts on human myeloid leukemia (HL60) cells and its mode of cell death

M. N. Norfazlina, M. Y. Farida Zuraina, N. F. Rajab, S. Mohd Nazip, A. R. Rumiza, C. F. Suziana Zaila, L. Lek Mun, N. Nurshahirah, L. Florinsiah

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human myeloid leukemia (HL60) cell line is the precursor and the attractive model of human cell for studying molecular event drug evaluation and cell differentiation. This research investigate the interactive effects between Nigella sativa (Ns) and Zingiber zerumbet (Zz) treated on HL60 cell line for 24 hours at non constant ratio of combination doses. To explain cytotoxicity effect of single and combination extracts is using 3-[45-dimethylthizol-2-yl]-25-diphenyltetrazolium bromide (MTT) assay. The combination of Ns and Zz at various combination doses setting showed the antagonism interactive effects. Ns and Zz showed more potent as anti proliferative agent when treated alone as compared to co-administrations of both. As single agent the IC50 of Ns (Petroleum ether extract) is 654.9ug/ml which is less cytotoxic as compared to Zz (Hexane extract) that inhibit 50% of HL60 viable cells at 63.72ug/ml (p<0.05). In conclusion combination between Ns and Zz conveyed the antagonist interactive effects and indicated that Ns and Zz could not be combined together in order to achieve a safe drug.

Original languageEnglish
Pages (from-to)51-55
Number of pages5
JournalJournal of Applied Pharmaceutical Science
Volume4
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

Nigella sativa
Myeloid Leukemia
HL-60 Cells
Cell Death
Cell Line
Drug Evaluation
Hexanes
Bromides
Inhibitory Concentration 50
In Vitro Techniques
Cell Differentiation
Research
Pharmaceutical Preparations

Keywords

  • 3-[45-dimethylthizol-2-yl]-25-diphenyltetrazolium bromide (MTT)
  • Apoptosis
  • Human myeloid leukemia (HL60)
  • Nigella sativa (Ns)
  • Zingiber zerumbet (Zz)

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Medicine (miscellaneous)
  • Pharmacology (medical)

Cite this

In vitro cytotoxicity effects of single and combination Nigella sativa and Zingiber zerumbet extracts on human myeloid leukemia (HL60) cells and its mode of cell death. / Norfazlina, M. N.; Farida Zuraina, M. Y.; Rajab, N. F.; Mohd Nazip, S.; Rumiza, A. R.; Suziana Zaila, C. F.; Lek Mun, L.; Nurshahirah, N.; Florinsiah, L.

In: Journal of Applied Pharmaceutical Science, Vol. 4, No. 5, 2014, p. 51-55.

Research output: Contribution to journalArticle

Norfazlina, M. N. ; Farida Zuraina, M. Y. ; Rajab, N. F. ; Mohd Nazip, S. ; Rumiza, A. R. ; Suziana Zaila, C. F. ; Lek Mun, L. ; Nurshahirah, N. ; Florinsiah, L. / In vitro cytotoxicity effects of single and combination Nigella sativa and Zingiber zerumbet extracts on human myeloid leukemia (HL60) cells and its mode of cell death. In: Journal of Applied Pharmaceutical Science. 2014 ; Vol. 4, No. 5. pp. 51-55.
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AU - Rajab, N. F.

AU - Mohd Nazip, S.

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