In vitro cytotoxic activity of new triphenyltin (IV) alkyl-isopropyldi-thiocarbamate compounds on human acute T-lymphoblastic cell line

Normah Awang, Nor Syaidatul Akmal Mohd Yousof, Nor Fadilah Rajab, Kamaludin Nurul Farahana

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cancer is one of the leading causes of mortality and morbidity worldwide. Various new metal-based compounds including organotin (IV) compounds have been actively synthesised due to their good cytotoxicity in cancerous cells. In this study, we tested the cytotoxicity of new triphenyltin (IV) dithiocarbamate compounds (triphenyltin (IV) benzylisopropyldithiocarbamate, compound 1; triphenyltin (IV) methylisopropyldithiocarbamate, compound 2; and triphenyltin (IV) ethylisopropyldithiocarbamate, compound 3) on the human acute T-lymphoblastic cell line, Jurkat E6.1 by MTT assay at three periods of time. The triphenyltin (IV) methylisopropyldithiocarbamate compound showed the lowest IC<inf>50</inf> values (0.03 μM) after 24 h of treatment on Jurkat E6.1 cell lines. The IC<inf>50</inf> values obtained for the three compounds for 24 h of treatment were 0.18 μM, 0.03 μM, and 0.42 μM, respectively. Next, for 48 h and 72 h of treatment, the IC<inf>50</inf> values were 0.15 μM, 0.18 μM, and 0.40 μM and 0.18 μM, 0.19 μM, and 0.41 μM, respectively. These tested compounds were found to give cytotoxic activity against Jurkat E6.1 cell lines at micromolar doses. Observation on morphological changes of Jurkat E6.1 cell lines treated with IC<inf>50</inf> values at 24 h of treatment showed morphological changes such as cell shrinkage and membrane blebbing, characterising the mode of cell death as apoptosis. Thus, further studies on the specific mechanisms of action of these compounds in human cells should be carried out to elucidate their potential as anticancer agents.

Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalJournal of Applied Pharmaceutical Science
Volume5
DOIs
Publication statusPublished - 2015

Fingerprint

Thiocarbamates
Inhibitory Concentration 50
Jurkat Cells
Cell Line
Organotin Compounds
Blister
Antineoplastic Agents
Cell Death
Metals
Cell Membrane
In Vitro Techniques
triphenyltin
Apoptosis
Morbidity
Mortality
Neoplasms

Keywords

  • Apoptosis
  • Cytotoxic
  • Dithiocarbamate
  • MTT assay
  • Triphenyltin(IV)

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Medicine (miscellaneous)
  • Pharmacology (medical)

Cite this

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title = "In vitro cytotoxic activity of new triphenyltin (IV) alkyl-isopropyldi-thiocarbamate compounds on human acute T-lymphoblastic cell line",
abstract = "Cancer is one of the leading causes of mortality and morbidity worldwide. Various new metal-based compounds including organotin (IV) compounds have been actively synthesised due to their good cytotoxicity in cancerous cells. In this study, we tested the cytotoxicity of new triphenyltin (IV) dithiocarbamate compounds (triphenyltin (IV) benzylisopropyldithiocarbamate, compound 1; triphenyltin (IV) methylisopropyldithiocarbamate, compound 2; and triphenyltin (IV) ethylisopropyldithiocarbamate, compound 3) on the human acute T-lymphoblastic cell line, Jurkat E6.1 by MTT assay at three periods of time. The triphenyltin (IV) methylisopropyldithiocarbamate compound showed the lowest IC50 values (0.03 μM) after 24 h of treatment on Jurkat E6.1 cell lines. The IC50 values obtained for the three compounds for 24 h of treatment were 0.18 μM, 0.03 μM, and 0.42 μM, respectively. Next, for 48 h and 72 h of treatment, the IC50 values were 0.15 μM, 0.18 μM, and 0.40 μM and 0.18 μM, 0.19 μM, and 0.41 μM, respectively. These tested compounds were found to give cytotoxic activity against Jurkat E6.1 cell lines at micromolar doses. Observation on morphological changes of Jurkat E6.1 cell lines treated with IC50 values at 24 h of treatment showed morphological changes such as cell shrinkage and membrane blebbing, characterising the mode of cell death as apoptosis. Thus, further studies on the specific mechanisms of action of these compounds in human cells should be carried out to elucidate their potential as anticancer agents.",
keywords = "Apoptosis, Cytotoxic, Dithiocarbamate, MTT assay, Triphenyltin(IV)",
author = "Normah Awang and Yousof, {Nor Syaidatul Akmal Mohd} and Rajab, {Nor Fadilah} and {Nurul Farahana}, Kamaludin",
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doi = "10.7324/JAPS.2015.54.S2",
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T1 - In vitro cytotoxic activity of new triphenyltin (IV) alkyl-isopropyldi-thiocarbamate compounds on human acute T-lymphoblastic cell line

AU - Awang, Normah

AU - Yousof, Nor Syaidatul Akmal Mohd

AU - Rajab, Nor Fadilah

AU - Nurul Farahana, Kamaludin

PY - 2015

Y1 - 2015

N2 - Cancer is one of the leading causes of mortality and morbidity worldwide. Various new metal-based compounds including organotin (IV) compounds have been actively synthesised due to their good cytotoxicity in cancerous cells. In this study, we tested the cytotoxicity of new triphenyltin (IV) dithiocarbamate compounds (triphenyltin (IV) benzylisopropyldithiocarbamate, compound 1; triphenyltin (IV) methylisopropyldithiocarbamate, compound 2; and triphenyltin (IV) ethylisopropyldithiocarbamate, compound 3) on the human acute T-lymphoblastic cell line, Jurkat E6.1 by MTT assay at three periods of time. The triphenyltin (IV) methylisopropyldithiocarbamate compound showed the lowest IC50 values (0.03 μM) after 24 h of treatment on Jurkat E6.1 cell lines. The IC50 values obtained for the three compounds for 24 h of treatment were 0.18 μM, 0.03 μM, and 0.42 μM, respectively. Next, for 48 h and 72 h of treatment, the IC50 values were 0.15 μM, 0.18 μM, and 0.40 μM and 0.18 μM, 0.19 μM, and 0.41 μM, respectively. These tested compounds were found to give cytotoxic activity against Jurkat E6.1 cell lines at micromolar doses. Observation on morphological changes of Jurkat E6.1 cell lines treated with IC50 values at 24 h of treatment showed morphological changes such as cell shrinkage and membrane blebbing, characterising the mode of cell death as apoptosis. Thus, further studies on the specific mechanisms of action of these compounds in human cells should be carried out to elucidate their potential as anticancer agents.

AB - Cancer is one of the leading causes of mortality and morbidity worldwide. Various new metal-based compounds including organotin (IV) compounds have been actively synthesised due to their good cytotoxicity in cancerous cells. In this study, we tested the cytotoxicity of new triphenyltin (IV) dithiocarbamate compounds (triphenyltin (IV) benzylisopropyldithiocarbamate, compound 1; triphenyltin (IV) methylisopropyldithiocarbamate, compound 2; and triphenyltin (IV) ethylisopropyldithiocarbamate, compound 3) on the human acute T-lymphoblastic cell line, Jurkat E6.1 by MTT assay at three periods of time. The triphenyltin (IV) methylisopropyldithiocarbamate compound showed the lowest IC50 values (0.03 μM) after 24 h of treatment on Jurkat E6.1 cell lines. The IC50 values obtained for the three compounds for 24 h of treatment were 0.18 μM, 0.03 μM, and 0.42 μM, respectively. Next, for 48 h and 72 h of treatment, the IC50 values were 0.15 μM, 0.18 μM, and 0.40 μM and 0.18 μM, 0.19 μM, and 0.41 μM, respectively. These tested compounds were found to give cytotoxic activity against Jurkat E6.1 cell lines at micromolar doses. Observation on morphological changes of Jurkat E6.1 cell lines treated with IC50 values at 24 h of treatment showed morphological changes such as cell shrinkage and membrane blebbing, characterising the mode of cell death as apoptosis. Thus, further studies on the specific mechanisms of action of these compounds in human cells should be carried out to elucidate their potential as anticancer agents.

KW - Apoptosis

KW - Cytotoxic

KW - Dithiocarbamate

KW - MTT assay

KW - Triphenyltin(IV)

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U2 - 10.7324/JAPS.2015.54.S2

DO - 10.7324/JAPS.2015.54.S2

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