In-vitro comparative study of marginal leakage and penetration ability of moisture-tolerant and conventional resin-based pit and fissure sealants with different surface preparations

Thavamalar Marimuthoo, Nor Atika Md Asfar, Mariati Abdul Rahman, S. Nagarajan M.P Sockalingam

Research output: Contribution to journalArticle

Abstract

This in-vitro study aimed to evaluate and compare the marginal leakage and penetration ability of a moisture-tolerant (Embrace WetBondTM) and a conventional (ClinproTM) resin-based sealants under three different enamel surface preparations (acid etched, acid etched and saliva contaminated and bur preparation and acid etched). One hundred and twenty extracted caries free human premolars teeth were cleaned and randomly divided into six groups of equal numbers, according to the type of sealants used and surface preparations. All the sealed teeth were subjected to thermocycling and immersed in a methylene blue dye. Each tooth was then embedded into acrylic resin before it was sectioned into four sections per tooth. Marginal leakage and unfilled surface area (indicating penetration depth of resin) were then measured using an optical 3D measurement device (Alicona Infinite Focus®). Both sealants exhibited comparable proportion of marginal leakage on acid etched only surfaces. Moisture-tolerant sealant showed the least proportion of marginal leakage on bur prepared and etched surfaces. Presence of saliva has detrimental effect on adhesion of both sealants. Nevertheless, depth of penetration of sealant into the fissures is comparable with both sealant types irrespective of the surface preparations.

Original languageEnglish
Pages (from-to)12-22
Number of pages11
JournalArchives of Orofacial Sciences
Volume12
Issue number1
Publication statusPublished - 1 Jun 2017

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Pit and Fissure Sealants
Tooth
Acids
Saliva
Acrylic Resins
Methylene Blue
Bicuspid
Dental Enamel
Coloring Agents
Equipment and Supplies
In Vitro Techniques

Keywords

  • Hydrophilic sealant
  • Marginal leakage
  • Penetration ability
  • Pit and fissure sealant

ASJC Scopus subject areas

  • Oral Surgery
  • Orthodontics

Cite this

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abstract = "This in-vitro study aimed to evaluate and compare the marginal leakage and penetration ability of a moisture-tolerant (Embrace WetBondTM) and a conventional (ClinproTM) resin-based sealants under three different enamel surface preparations (acid etched, acid etched and saliva contaminated and bur preparation and acid etched). One hundred and twenty extracted caries free human premolars teeth were cleaned and randomly divided into six groups of equal numbers, according to the type of sealants used and surface preparations. All the sealed teeth were subjected to thermocycling and immersed in a methylene blue dye. Each tooth was then embedded into acrylic resin before it was sectioned into four sections per tooth. Marginal leakage and unfilled surface area (indicating penetration depth of resin) were then measured using an optical 3D measurement device (Alicona Infinite Focus{\circledR}). Both sealants exhibited comparable proportion of marginal leakage on acid etched only surfaces. Moisture-tolerant sealant showed the least proportion of marginal leakage on bur prepared and etched surfaces. Presence of saliva has detrimental effect on adhesion of both sealants. Nevertheless, depth of penetration of sealant into the fissures is comparable with both sealant types irrespective of the surface preparations.",
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