In vitro chondrogenesis transformation study of mouse dental pulp stem cells

Shahrul Hisham Zainal Ariffin, Shabnam Kermani, Rohaya Megat Abdul Wahab, Sahidan Senafi, Zaidah Zainal Ariffin, Mohamad Abdul Razak

Research output: Contribution to journalArticle

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Abstract

A major challenge in the application of mesenchymal stem cells in cartilage reconstruction is that whether the cells are able to differentiate into fully mature chondrocytes before grafting. The aim of this study was to isolate mouse dental pulp stem cells (DPSC) and differentiate them into chondrocytes. For this investigation, morphological, molecular, and biochemical analyses for differentiated cells were used. To induce the chondrocyte differentiation, DPSC were cultured in chondrogenic medium (Zen-Bio, Inc.). Based on morphological analyses using toluidine blue staining, proteoglycan products appear in DPSC after 21 days of chondrocyte induction. Biochemical analyses in differentiated group showed that alkaline phosphatase activity was significantly increased at day 14 as compared to control (P < 0.05). Cell viability analyses during the differentiation to chondrocytes also showed that these cells were viable during differentiation. However, after the 14th day of differentiation, there was a significant decrease (P < 0.05) in the viability proportion among differentiated cells as compared to the control cells. In RT-PCR molecular analyses, mouse DPSC expressed Cd146 and Cd166 which indicated that these cells belong to mesenchymal stem cells. Coll I and Coll II markers showed high expression after 14 and 21 days, respectively. In conclusion, this study showed that DPSC successfully differentiated into chondrocytes.

Original languageEnglish
Article number827149
JournalThe Scientific World Journal
Volume2012
DOIs
Publication statusPublished - 2012

Fingerprint

Chondrogenesis
Dental Pulp
Chondrocytes
Stem cells
Pulp
Stem Cells
stem
Mesenchymal Stromal Cells
viability
Tolonium Chloride
Proteoglycans
Cartilage
Alkaline Phosphatase
In Vitro Techniques
pulp
Cell Survival
phosphatase
Staining and Labeling
Cells
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

In vitro chondrogenesis transformation study of mouse dental pulp stem cells. / Zainal Ariffin, Shahrul Hisham; Kermani, Shabnam; Megat Abdul Wahab, Rohaya; Senafi, Sahidan; Zainal Ariffin, Zaidah; Abdul Razak, Mohamad.

In: The Scientific World Journal, Vol. 2012, 827149, 2012.

Research output: Contribution to journalArticle

Zainal Ariffin, Shahrul Hisham ; Kermani, Shabnam ; Megat Abdul Wahab, Rohaya ; Senafi, Sahidan ; Zainal Ariffin, Zaidah ; Abdul Razak, Mohamad. / In vitro chondrogenesis transformation study of mouse dental pulp stem cells. In: The Scientific World Journal. 2012 ; Vol. 2012.
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