In vitro characterization of pluronic F127 and D-α-tocopheryl polyethylene glycol 1000 succinate mixed micelles as nanocarriers for targeted anticancer-drug delivery

Adeel Masood Butt, Mohd Cairul Iqbal Mohd Amin, Haliza Katas, Narong Sarisuta, Wasu Witoonsaridsilp, Ruthairat Benjakul

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Mixed micelles of Pluronic F127 and d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) in different molar ratios (10: 0, 7: 3, 5: 5, and 3: 7) were prepared to characterize this system as nanocarriers for targeted delivery of chemotherapeutic agents. Their size, zeta potential, critical micelle concentration, drug loading content, entrapment efficiency, drug release, cytotoxicity, and stability in serum were evaluated in vitro by using doxorubicin as the model anticancer drug. The micellar sizes ranged from 25 to 35 nm. The 7: 3 and 5: 5 micellar combinations had lower critical micelle concentrations (5×10-5 M) than the 10: 0 combination (5×10-4 M). The entrapment efficiencies of the 7: 3, 5: 5, and 3: 7 micellar combinations were 72, 88, and 69, respectively. Doxorubicin release was greater at acidic tumour pH than at normal physiological pH. The doxorubicin-loaded mixed micelles showed greater percent inhibition and apoptosis activity in human breast adenocarcinoma (MCF-7) and acute monocytic leukaemia (THP-1) cell lines than free doxorubicin did. The mixed micelles were also stable against aggregation and precipitation in serum. These findings suggest that Pluronic F127-TPGS mixed micelles could be used as nanocarriers for targeted anticancer-drug delivery.

Original languageEnglish
Article number916573
JournalJournal of Nanomaterials
Volume2012
DOIs
Publication statusPublished - 2012

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UCON 50-HB-5100
Poloxamer
Micelles
Succinic Acid
Doxorubicin
Polyethylene glycols
Critical micelle concentration
Pharmaceutical Preparations
Cell death
Zeta potential
Cytotoxicity
Tumors
Agglomeration
Cells
Apoptosis
Targeted drug delivery
polyethylene glycol 1000

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

In vitro characterization of pluronic F127 and D-α-tocopheryl polyethylene glycol 1000 succinate mixed micelles as nanocarriers for targeted anticancer-drug delivery. / Butt, Adeel Masood; Mohd Amin, Mohd Cairul Iqbal; Katas, Haliza; Sarisuta, Narong; Witoonsaridsilp, Wasu; Benjakul, Ruthairat.

In: Journal of Nanomaterials, Vol. 2012, 916573, 2012.

Research output: Contribution to journalArticle

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AU - Witoonsaridsilp, Wasu

AU - Benjakul, Ruthairat

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