In-vitro characterization of lytic bacteriophage PhVh6 as potential biocontrol agent against pathogenic Vibrio harveyi

Wan Wan Ibrahim Nurhafizah, Syafiqah Aznan Alia, Nur Saari Azna, Kok Leong Lee, Musa Nadirah, Abdul Razzak Laith, Muhd Danish-Daniel, Sandra Catherine Zainathan, Mohamed Mohamed Din Shariff, Mazlan Abd. Ghaffar, Musa Najiah

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Bacterial diseases are the major threat to the shrimp aquaculture industry. However, the applications of antibiotics for treatment and prophylaxis are generally not permissible by law. The present study characterized a lytic bacteriophage strain PhVh6 for biocontrol potential against pathogenic Vibrio spp. PhVh6 was isolated from the water in shrimp pond encountered ongoing mortality using luminescent Vibrio harveyi strain Vh10 (GenBank: KJ700303) as enrichment host. Characterizations were conducted for morphology, titer (pfu mL–1), multiplicity of infection (MOI), onestep growth curve, physico-chemical sensitivities, host range susceptibility and bacteriolytic activities against Vibrio spp. Transmission electron microscopy revealed tailed icosahedral viral particles lacking of neck and collar, thus assigned PhVh6 to the order Caudovirales and family Siphoviridae. MOI of 1 was found optimum for PhVh6 and Vh10 interaction. One-step growth curve analysis revealed short latent period and high burst rate. PhVh6 was stable from 25 to 65°C, and optimum at 45°C. PhVh6 was able to tolerate low temperatures at 4, –20 and –75°C. The lytic ability was optimum at pH 6 to 8. PhVh6 was tolerant to salinities of 15, 30 and 45 ppt, 1–3% chloroform and 30–40% glycerol. Host range susceptibility test showed ability of PhVh6 to lyse Vibrio parahaemolyticus, Vibrio alginolyticus and Vibrio harveyi strain Vh8. The present study suggested the potential of PhVh6 as biocontrol agent against Vibrio spp. in shrimp aquaculture.

Original languageEnglish
Pages (from-to)64-76
Number of pages13
JournalAACL Bioflux
Volume10
Issue number1
Publication statusPublished - 28 Feb 2017

Fingerprint

potential biocontrol agent
Vibrio harveyi
host range
bacteriophage
growth curve
Vibrio
bacteriophages
biological control agents
shrimp culture
bacterial disease
aquaculture industry
biocontrol agent
Caudovirales
lysis
chloroform
Siphoviridae
antibiotics
shrimp fisheries
aquaculture
transmission electron microscopy

Keywords

  • Biocontrol
  • Caudovirales
  • Lytic bacteriophage
  • Shrimp aquaculture
  • Vibrio harveyi

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Water Science and Technology
  • Management, Monitoring, Policy and Law

Cite this

Nurhafizah, W. W. I., Alia, S. A., Azna, N. S., Lee, K. L., Nadirah, M., Laith, A. R., ... Najiah, M. (2017). In-vitro characterization of lytic bacteriophage PhVh6 as potential biocontrol agent against pathogenic Vibrio harveyi. AACL Bioflux, 10(1), 64-76.

In-vitro characterization of lytic bacteriophage PhVh6 as potential biocontrol agent against pathogenic Vibrio harveyi. / Nurhafizah, Wan Wan Ibrahim; Alia, Syafiqah Aznan; Azna, Nur Saari; Lee, Kok Leong; Nadirah, Musa; Laith, Abdul Razzak; Danish-Daniel, Muhd; Zainathan, Sandra Catherine; Shariff, Mohamed Mohamed Din; Abd. Ghaffar, Mazlan; Najiah, Musa.

In: AACL Bioflux, Vol. 10, No. 1, 28.02.2017, p. 64-76.

Research output: Contribution to journalArticle

Nurhafizah, WWI, Alia, SA, Azna, NS, Lee, KL, Nadirah, M, Laith, AR, Danish-Daniel, M, Zainathan, SC, Shariff, MMD, Abd. Ghaffar, M & Najiah, M 2017, 'In-vitro characterization of lytic bacteriophage PhVh6 as potential biocontrol agent against pathogenic Vibrio harveyi', AACL Bioflux, vol. 10, no. 1, pp. 64-76.
Nurhafizah WWI, Alia SA, Azna NS, Lee KL, Nadirah M, Laith AR et al. In-vitro characterization of lytic bacteriophage PhVh6 as potential biocontrol agent against pathogenic Vibrio harveyi. AACL Bioflux. 2017 Feb 28;10(1):64-76.
Nurhafizah, Wan Wan Ibrahim ; Alia, Syafiqah Aznan ; Azna, Nur Saari ; Lee, Kok Leong ; Nadirah, Musa ; Laith, Abdul Razzak ; Danish-Daniel, Muhd ; Zainathan, Sandra Catherine ; Shariff, Mohamed Mohamed Din ; Abd. Ghaffar, Mazlan ; Najiah, Musa. / In-vitro characterization of lytic bacteriophage PhVh6 as potential biocontrol agent against pathogenic Vibrio harveyi. In: AACL Bioflux. 2017 ; Vol. 10, No. 1. pp. 64-76.
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