In vitro biocompatibility evaluation of poly(3-hydroxybutyrate-co-4- hydroxybutyrate) copolymer in fibroblast cells

Ling Siew Ee, Nor Fadilah Rajab, Annuar Bin Osman, Kumar Sudesh, Salmaan Hussain Inayat-Hussain

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Among the various biomaterials available for tissue engineering and therapeutic applications, microbial polyhydroxyalkanoates offer the most diverse range of thermal and mechanical properties. In this study, the biocompatibility of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB); containing 50 mol % of 4-hydroxybutyrate] copolymer produced by Delftia acidovorans was evaluated. The cytotoxicity, mode of cell death, and genotoxicity of P(3HB-co-4HB) extract against V79 and L929 fibroblast cells were assessed using MTT assay, acridine orange/propidium iodide staining, and alkaline comet assay, respectively. Our results demonstrate that P(3HB-co-4HB) treated on both cell lines were comparable with clinically-used Polyglactin 910, where more man 60% of viable cells were observed following 72-h treatment at 200 mg/mL. Further morphological investigation on the mode of cell death showed an increase in apoptotic cells in a time-dependent manner in both cell lines. On the other hand, P(3HB-co-4HB) at 200 mg/mL showed no genotoxic effects as determined by alkaline comet assay following 72-h treatment. In conclusion, our study indicated that P(3HB-co-4HB) compounds showed good biocompatibility in fibroblast cells suggesting that it has potential to be used for future medical applications.

Original languageEnglish
Pages (from-to)317-325
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume81
Issue number2
DOIs
Publication statusPublished - May 2007

Fingerprint

Hydroxybutyrates
Fibroblasts
Biocompatibility
3-Hydroxybutyric Acid
Copolymers
Cells
Assays
Cell death
Polyhydroxyalkanoates
Polyglactin 910
Acridine Orange
Propidium
Biocompatible Materials
Medical applications
Cytotoxicity
Tissue engineering
Biomaterials
Thermodynamic properties
poly(3-hydroxybutyrate-co-4-hydroxybutyrate)
Mechanical properties

Keywords

  • Apoptosis
  • Cytotoxicity
  • Fibroblasts
  • Genotoxicity
  • Polyhydroxyalkanoates

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

In vitro biocompatibility evaluation of poly(3-hydroxybutyrate-co-4- hydroxybutyrate) copolymer in fibroblast cells. / Ee, Ling Siew; Rajab, Nor Fadilah; Osman, Annuar Bin; Sudesh, Kumar; Inayat-Hussain, Salmaan Hussain.

In: Journal of Biomedical Materials Research - Part A, Vol. 81, No. 2, 05.2007, p. 317-325.

Research output: Contribution to journalArticle

Ee, Ling Siew ; Rajab, Nor Fadilah ; Osman, Annuar Bin ; Sudesh, Kumar ; Inayat-Hussain, Salmaan Hussain. / In vitro biocompatibility evaluation of poly(3-hydroxybutyrate-co-4- hydroxybutyrate) copolymer in fibroblast cells. In: Journal of Biomedical Materials Research - Part A. 2007 ; Vol. 81, No. 2. pp. 317-325.
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