In vitro antiviral activity of styrylpyrone derivative-incorporated formulations against Herpes simplex virus type-1

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Abstract

Aims: This study was aimed to evaluate in vitro antiviral activity of topical formulations incorporated with a styrylpyrone derivative (SPD) against Herpes Simplex Virus type 1 (HSV-1). Methodology and results: Two types of SPD-incorporated formulations (ointment and gel) were tested for their antiviral activity against HSV-1 clinical strain using plaque reduction assay on Vero cells. The antiviral activity was determined based on the percentage of plaque reduction occurred between treatment and control (non-treated infected cells). In this study, 10% SPD-gel (SPD = 0.004 mg) and 20% SPD-ointment (SPD = 0.003 mg) showed plaque reduction percentage of 87% and 79% respectively. Further evaluation on the ointment base, gel base (formulation without SPD) demonstrated less than 10% of antiviral activity while pure SPD at 0.0025 mg showed 81% of plaque reduction. These results indicated that the antiviral activity observed in both SPD-incorporated ointment and gel was mainly due to SPD regardless of formulation components. Furthermore, the antiviral activities observed in both SPD-incorporated products were also in agreement with the antiviral activity observed in pure SPD. Conclusion, significance and impact study: SPD-incorporated products retained the antiviral activity and can further be tested in animal model.

Original languageEnglish
Pages (from-to)228-232
Number of pages5
JournalMalaysian Journal of Microbiology
Volume12
Issue number3
DOIs
Publication statusPublished - 2016

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Human Herpesvirus 1
Antiviral Agents
Ointments
Gels
Ointment Bases
Vero Cells
In Vitro Techniques
Animal Models

Keywords

  • Antiviral
  • Goniothalamus umbrosus
  • HSV-1
  • Styrylpyrone derivative

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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title = "In vitro antiviral activity of styrylpyrone derivative-incorporated formulations against Herpes simplex virus type-1",
abstract = "Aims: This study was aimed to evaluate in vitro antiviral activity of topical formulations incorporated with a styrylpyrone derivative (SPD) against Herpes Simplex Virus type 1 (HSV-1). Methodology and results: Two types of SPD-incorporated formulations (ointment and gel) were tested for their antiviral activity against HSV-1 clinical strain using plaque reduction assay on Vero cells. The antiviral activity was determined based on the percentage of plaque reduction occurred between treatment and control (non-treated infected cells). In this study, 10{\%} SPD-gel (SPD = 0.004 mg) and 20{\%} SPD-ointment (SPD = 0.003 mg) showed plaque reduction percentage of 87{\%} and 79{\%} respectively. Further evaluation on the ointment base, gel base (formulation without SPD) demonstrated less than 10{\%} of antiviral activity while pure SPD at 0.0025 mg showed 81{\%} of plaque reduction. These results indicated that the antiviral activity observed in both SPD-incorporated ointment and gel was mainly due to SPD regardless of formulation components. Furthermore, the antiviral activities observed in both SPD-incorporated products were also in agreement with the antiviral activity observed in pure SPD. Conclusion, significance and impact study: SPD-incorporated products retained the antiviral activity and can further be tested in animal model.",
keywords = "Antiviral, Goniothalamus umbrosus, HSV-1, Styrylpyrone derivative",
author = "Bahtiar, {Adibah Ahamad} and {Md Nor}, {Norefrina Shafinaz} and Nazlina Ibrahim",
year = "2016",
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language = "English",
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pages = "228--232",
journal = "Malaysian Journal of Microbiology",
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AU - Bahtiar, Adibah Ahamad

AU - Md Nor, Norefrina Shafinaz

AU - Ibrahim, Nazlina

PY - 2016

Y1 - 2016

N2 - Aims: This study was aimed to evaluate in vitro antiviral activity of topical formulations incorporated with a styrylpyrone derivative (SPD) against Herpes Simplex Virus type 1 (HSV-1). Methodology and results: Two types of SPD-incorporated formulations (ointment and gel) were tested for their antiviral activity against HSV-1 clinical strain using plaque reduction assay on Vero cells. The antiviral activity was determined based on the percentage of plaque reduction occurred between treatment and control (non-treated infected cells). In this study, 10% SPD-gel (SPD = 0.004 mg) and 20% SPD-ointment (SPD = 0.003 mg) showed plaque reduction percentage of 87% and 79% respectively. Further evaluation on the ointment base, gel base (formulation without SPD) demonstrated less than 10% of antiviral activity while pure SPD at 0.0025 mg showed 81% of plaque reduction. These results indicated that the antiviral activity observed in both SPD-incorporated ointment and gel was mainly due to SPD regardless of formulation components. Furthermore, the antiviral activities observed in both SPD-incorporated products were also in agreement with the antiviral activity observed in pure SPD. Conclusion, significance and impact study: SPD-incorporated products retained the antiviral activity and can further be tested in animal model.

AB - Aims: This study was aimed to evaluate in vitro antiviral activity of topical formulations incorporated with a styrylpyrone derivative (SPD) against Herpes Simplex Virus type 1 (HSV-1). Methodology and results: Two types of SPD-incorporated formulations (ointment and gel) were tested for their antiviral activity against HSV-1 clinical strain using plaque reduction assay on Vero cells. The antiviral activity was determined based on the percentage of plaque reduction occurred between treatment and control (non-treated infected cells). In this study, 10% SPD-gel (SPD = 0.004 mg) and 20% SPD-ointment (SPD = 0.003 mg) showed plaque reduction percentage of 87% and 79% respectively. Further evaluation on the ointment base, gel base (formulation without SPD) demonstrated less than 10% of antiviral activity while pure SPD at 0.0025 mg showed 81% of plaque reduction. These results indicated that the antiviral activity observed in both SPD-incorporated ointment and gel was mainly due to SPD regardless of formulation components. Furthermore, the antiviral activities observed in both SPD-incorporated products were also in agreement with the antiviral activity observed in pure SPD. Conclusion, significance and impact study: SPD-incorporated products retained the antiviral activity and can further be tested in animal model.

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KW - Goniothalamus umbrosus

KW - HSV-1

KW - Styrylpyrone derivative

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