In Vitro Antifungal Susceptibility of Neoscytalidium dimidiatum Clinical Isolates from Malaysia

Jasper Elvin James, Jacinta Santhanam, Mei Chen Lee, Choon Xian Wong, Parameswari Sabaratnam, Hamidah Yusoff, Tzar Mohd Nizam Khaithir, Mohd Fuat Abdul Razak

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Neoscytalidium dimidiatum is an opportunistic fungus causing cutaneous infections mostly, which are difficult to treat due to antifungal resistance. In Malaysia, N. dimidiatum is associated with skin and nail infections, especially in the elderly. These infections may be mistaken for dermatophyte infections due to similar clinical appearance. In this study, Neoscytalidium isolates from cutaneous specimens, identified using morphological and molecular methods (28 Neoscytalidium dimidiatum and 1 Neoscytalidium sp.), were evaluated for susceptibility towards antifungal agents using the CLSI broth microdilution (M38-A2) and Etest methods. Amphotericin B, voriconazole, miconazole and clotrimazole showed high in vitro activity against all isolates with MIC ranging from 0.0313 to 1 µg/mL. Susceptibility towards fluconazole and itraconazole was noted in up to 10% of isolates, while ketoconazole was inactive against all isolates. Clinical breakpoints for antifungal drugs are not yet available for most filamentous fungi, including Neoscytalidium species. However, the results indicate that clinical isolates of N. dimidiatum in Malaysia were sensitive towards miconazole, clotrimazole, voriconazole and amphotericin B, in vitro.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalMycopathologia
DOIs
Publication statusAccepted/In press - 4 Nov 2016

Fingerprint

Neoscytalidium
Malaysia
Clotrimazole
Miconazole
Arthrodermataceae
clotrimazole
miconazole
Amphotericin B
Infection
antifungal agents
amphotericin B
Disk Diffusion Antimicrobial Tests
infection
Skin
Ketoconazole
Itraconazole
Fluconazole
Antifungal Agents
Nails
ketoconazole

Keywords

  • Antifungal susceptibility
  • Malaysia
  • Neoscytalidium dimidiatum

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • veterinary (miscalleneous)
  • Agronomy and Crop Science
  • Plant Science

Cite this

James, J. E., Santhanam, J., Lee, M. C., Wong, C. X., Sabaratnam, P., Yusoff, H., ... Razak, M. F. A. (Accepted/In press). In Vitro Antifungal Susceptibility of Neoscytalidium dimidiatum Clinical Isolates from Malaysia. Mycopathologia, 1-9. https://doi.org/10.1007/s11046-016-0085-5

In Vitro Antifungal Susceptibility of Neoscytalidium dimidiatum Clinical Isolates from Malaysia. / James, Jasper Elvin; Santhanam, Jacinta; Lee, Mei Chen; Wong, Choon Xian; Sabaratnam, Parameswari; Yusoff, Hamidah; Khaithir, Tzar Mohd Nizam; Razak, Mohd Fuat Abdul.

In: Mycopathologia, 04.11.2016, p. 1-9.

Research output: Contribution to journalArticle

James, Jasper Elvin ; Santhanam, Jacinta ; Lee, Mei Chen ; Wong, Choon Xian ; Sabaratnam, Parameswari ; Yusoff, Hamidah ; Khaithir, Tzar Mohd Nizam ; Razak, Mohd Fuat Abdul. / In Vitro Antifungal Susceptibility of Neoscytalidium dimidiatum Clinical Isolates from Malaysia. In: Mycopathologia. 2016 ; pp. 1-9.
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