In vitro antifungal activities against moulds isolated from dermatological specimens

Tzar Mohd Nizam Khaithir, Rabiatul Adawiyah A G Binting, Shafika Mohd Saari, Thivyananthini Vijaya Kumar, Marianayati Muhammad, Hartini Satim, Hamidah Yusoff, Jacinta Santhanam

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: This study aimed to determine the minimum inhibitory concentrations (MICs) of various antifungal agents against moulds isolated from dermatological specimens. Methods: We identified 29 moulds from dermatological specimens between October 2012 and March 2013 by conventional methods. We performed antifungal susceptibility testing on six antifungal agents, amphotericin B, clotrimazole, itraconazole, ketoconazole, miconazole and terbinafine, according to the Clinical and Laboratory Standards Institute guidelines contained in the M38-A2 document. Results: Most antifungal agents were active against the dermatophytes, except for terbinafine against Trichophyton rubrum (geometric mean MIC, MICGM 3.17 μg/mL). The dematiaceous moulds were relatively susceptible to amphotericin B and azoles (MICGM 0.17-0.34 μg/mL), but not to terbinafine (MICGM 3.62 μg/mL). Septate hyaline moulds showed variable results between the relatively more susceptible Aspergillus spp. (MICGM 0.25-4 μg/mL) and the more resistant Fusarium spp. (MICGM 5.66-32 μg/mL). The zygomycetes were susceptible to amphotericin B (MICGM 0.5 μg/ mL) and clotrimazole (MICGM 0.08 μg/mL), but not to other azoles (MICGM 2.52-4 μg/mL). Conclusion: Amphotericin B and clotrimazole were the most effective antifungal agents against all moulds excepting Fusarium spp., while terbinafine was useful against dermatophytes (except T. rubrum) and Aspergillus spp. However, a larger study is required to draw more solid conclusions.

Original languageEnglish
Pages (from-to)32-39
Number of pages8
JournalMalaysian Journal of Medical Sciences
Volume23
Issue number3
Publication statusPublished - 1 May 2016

Fingerprint

terbinafine
Antifungal Agents
Amphotericin B
Clotrimazole
Fungi
Azoles
Arthrodermataceae
Fusarium
Microbial Sensitivity Tests
Aspergillus
Miconazole
Trichophyton
Hyalin
Ketoconazole
Itraconazole
varespladib methyl
In Vitro Techniques
Guidelines

Keywords

  • Amphotericin B
  • Antifungal
  • Azoles
  • Dermatology
  • Mold
  • Terbinafine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

In vitro antifungal activities against moulds isolated from dermatological specimens. / Khaithir, Tzar Mohd Nizam; Binting, Rabiatul Adawiyah A G; Mohd Saari, Shafika; Kumar, Thivyananthini Vijaya; Muhammad, Marianayati; Satim, Hartini; Yusoff, Hamidah; Santhanam, Jacinta.

In: Malaysian Journal of Medical Sciences, Vol. 23, No. 3, 01.05.2016, p. 32-39.

Research output: Contribution to journalArticle

Khaithir, TMN, Binting, RAAG, Mohd Saari, S, Kumar, TV, Muhammad, M, Satim, H, Yusoff, H & Santhanam, J 2016, 'In vitro antifungal activities against moulds isolated from dermatological specimens', Malaysian Journal of Medical Sciences, vol. 23, no. 3, pp. 32-39.
Khaithir TMN, Binting RAAG, Mohd Saari S, Kumar TV, Muhammad M, Satim H et al. In vitro antifungal activities against moulds isolated from dermatological specimens. Malaysian Journal of Medical Sciences. 2016 May 1;23(3):32-39.
Khaithir, Tzar Mohd Nizam ; Binting, Rabiatul Adawiyah A G ; Mohd Saari, Shafika ; Kumar, Thivyananthini Vijaya ; Muhammad, Marianayati ; Satim, Hartini ; Yusoff, Hamidah ; Santhanam, Jacinta. / In vitro antifungal activities against moulds isolated from dermatological specimens. In: Malaysian Journal of Medical Sciences. 2016 ; Vol. 23, No. 3. pp. 32-39.
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abstract = "Background: This study aimed to determine the minimum inhibitory concentrations (MICs) of various antifungal agents against moulds isolated from dermatological specimens. Methods: We identified 29 moulds from dermatological specimens between October 2012 and March 2013 by conventional methods. We performed antifungal susceptibility testing on six antifungal agents, amphotericin B, clotrimazole, itraconazole, ketoconazole, miconazole and terbinafine, according to the Clinical and Laboratory Standards Institute guidelines contained in the M38-A2 document. Results: Most antifungal agents were active against the dermatophytes, except for terbinafine against Trichophyton rubrum (geometric mean MIC, MICGM 3.17 μg/mL). The dematiaceous moulds were relatively susceptible to amphotericin B and azoles (MICGM 0.17-0.34 μg/mL), but not to terbinafine (MICGM 3.62 μg/mL). Septate hyaline moulds showed variable results between the relatively more susceptible Aspergillus spp. (MICGM 0.25-4 μg/mL) and the more resistant Fusarium spp. (MICGM 5.66-32 μg/mL). The zygomycetes were susceptible to amphotericin B (MICGM 0.5 μg/ mL) and clotrimazole (MICGM 0.08 μg/mL), but not to other azoles (MICGM 2.52-4 μg/mL). Conclusion: Amphotericin B and clotrimazole were the most effective antifungal agents against all moulds excepting Fusarium spp., while terbinafine was useful against dermatophytes (except T. rubrum) and Aspergillus spp. However, a larger study is required to draw more solid conclusions.",
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