In vitro anti-malarial activity of a plant extract from Malaysia

Mohamed Kamel Abdul Ghani, M. J Siti Najila, S. I Syed Zahir, A. Noor Rain, Lokman Hakim, I. Zakiah, Khozirah, Azizol

Research output: Contribution to journalArticle

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Abstract

Introduction: Malaria remains as one of the leading causes of illness and death in the world. This could be attributed in part to the widespread problem of parasite drug resistance. The quest for new drugs has led researchers focussing on medicinal plants in the hope that they can provide novel anti-malarial agents. Objective: In this preliminary study, a plant from Malaysia, Goniothalamus scortechinii was selected based on its traditional values and claims, and screened for anti-malarial activity towards Plasmodium falciparum. Materials and Methods: Two strains of Plasmodium falciparum, the chloroquine resistant, Gombak A and the sensitive strain, D10 were screened for anti-malarial activity towards Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. Results: The crude extract of G. scortechinii exhibited potent schizonticidal activity. It is effective against both the chloroquine resistant, Gombak A and the sensitive strain, D10 of Plasmodium falciparum. A better IC50 value was obtained against the resistant strain, (9 μg/ml) compared to the sensitive strain, 40 μg/ml. When the crude extract was fractionated into 3 fractions, the chloroform fraction yielded the best activity, exhibiting equipotency against both strains of parasite used; IC50 of 21.06 μg/mi against Gombak A and 23.53 μg/ml against D10. Conclusion: G. scortechinii exhibited potent schizonticidal activity and is effective against both the chloroquine resistant, Gombak A and the sensitive strain, D10 of Plasmodium falciparum. This plant should make a good potential source of new anti-malarial drug.

Original languageEnglish
Pages (from-to)135-138
Number of pages4
JournalInternational Medical Journal
Volume9
Issue number2
Publication statusPublished - Jun 2002

Fingerprint

Malaysia
Plant Extracts
Antimalarials
Plasmodium falciparum
Chloroquine
Complex Mixtures
Inhibitory Concentration 50
Goniothalamus
Parasites
Medicinal Plants
Chloroform
L-Lactate Dehydrogenase
Drug Resistance
Pharmaceutical Preparations
Malaria
Cause of Death
Research Personnel
In Vitro Techniques

Keywords

  • Goniothalamus scortechinii
  • In vitro anti-malarial activity
  • Lactate dehydrogenase (LDH) assay
  • Medicinal plants
  • Plasmodiumfalciparum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Abdul Ghani, M. K., Najila, M. J. S., Zahir, S. I. S., Rain, A. N., Hakim, L., Zakiah, I., ... Azizol (2002). In vitro anti-malarial activity of a plant extract from Malaysia. International Medical Journal, 9(2), 135-138.

In vitro anti-malarial activity of a plant extract from Malaysia. / Abdul Ghani, Mohamed Kamel; Najila, M. J Siti; Zahir, S. I Syed; Rain, A. Noor; Hakim, Lokman; Zakiah, I.; Khozirah; Azizol.

In: International Medical Journal, Vol. 9, No. 2, 06.2002, p. 135-138.

Research output: Contribution to journalArticle

Abdul Ghani, MK, Najila, MJS, Zahir, SIS, Rain, AN, Hakim, L, Zakiah, I, Khozirah & Azizol 2002, 'In vitro anti-malarial activity of a plant extract from Malaysia', International Medical Journal, vol. 9, no. 2, pp. 135-138.
Abdul Ghani MK, Najila MJS, Zahir SIS, Rain AN, Hakim L, Zakiah I et al. In vitro anti-malarial activity of a plant extract from Malaysia. International Medical Journal. 2002 Jun;9(2):135-138.
Abdul Ghani, Mohamed Kamel ; Najila, M. J Siti ; Zahir, S. I Syed ; Rain, A. Noor ; Hakim, Lokman ; Zakiah, I. ; Khozirah ; Azizol. / In vitro anti-malarial activity of a plant extract from Malaysia. In: International Medical Journal. 2002 ; Vol. 9, No. 2. pp. 135-138.
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AU - Zahir, S. I Syed

AU - Rain, A. Noor

AU - Hakim, Lokman

AU - Zakiah, I.

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N2 - Introduction: Malaria remains as one of the leading causes of illness and death in the world. This could be attributed in part to the widespread problem of parasite drug resistance. The quest for new drugs has led researchers focussing on medicinal plants in the hope that they can provide novel anti-malarial agents. Objective: In this preliminary study, a plant from Malaysia, Goniothalamus scortechinii was selected based on its traditional values and claims, and screened for anti-malarial activity towards Plasmodium falciparum. Materials and Methods: Two strains of Plasmodium falciparum, the chloroquine resistant, Gombak A and the sensitive strain, D10 were screened for anti-malarial activity towards Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. Results: The crude extract of G. scortechinii exhibited potent schizonticidal activity. It is effective against both the chloroquine resistant, Gombak A and the sensitive strain, D10 of Plasmodium falciparum. A better IC50 value was obtained against the resistant strain, (9 μg/ml) compared to the sensitive strain, 40 μg/ml. When the crude extract was fractionated into 3 fractions, the chloroform fraction yielded the best activity, exhibiting equipotency against both strains of parasite used; IC50 of 21.06 μg/mi against Gombak A and 23.53 μg/ml against D10. Conclusion: G. scortechinii exhibited potent schizonticidal activity and is effective against both the chloroquine resistant, Gombak A and the sensitive strain, D10 of Plasmodium falciparum. This plant should make a good potential source of new anti-malarial drug.

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