In vitro anti-allergic activity of Moringa oleifera Lam. extracts and their isolated compounds

Nur Zahirah Abd Rani, Endang Kumolosasi, Malina Jasamai, Jamia Azdina Jamal, Kok Wai Lam, Khairana Husain

Research output: Contribution to journalArticle

Abstract

Background: Moringa oleifera Lam. is a commonly used plant in herbal medicine and has various reported bioactivities such as antioxidant, antimicrobial, anticancer and antidiabetes. It is rich in nutrients and polyphenols. The plant also has been traditionally used for alleviating allergic conditions. This study was aimed to examine the anti-allergic activity of M. oleifera extracts and its isolated compounds. Method: M. oleifera leaves, seeds and pods were extracted with 80% of ethanol. Individual compounds were isolated using a column chromatographic technique and elucidated based on the nuclear magnetic resonance (NMR) and electrospray ionisation mass spectrometry (ESIMS) spectral data. The anti-allergic activity of the extracts, isolated compounds and ketotifen fumarate as a positive control was evaluated using rat basophilic leukaemia (RBL-2H3) cells for early and late phases of allergic reactions. The early phase was determined based on the inhibition of beta-hexosaminidase and histamine release; while the late phase was based on the inhibition of interleukin (IL-4) and tumour necrosis factor (TNF-α) release. Results: Two new compounds; ethyl-(E)-undec-6-enoate (1) and 3,5,6-trihydroxy-2-(2,3,4,5,6-pentahydroxyphenyl)-4H-chromen-4-one (2) together with six known compounds; quercetin (3), kaempferol (4), β-sitosterol-3-O-glucoside (5), oleic acid (6), glucomoringin (7), 2,3,4-trihydroxybenzaldehyde (8) and stigmasterol (9) were isolated from M. oleifera extracts. All extracts and the isolated compounds inhibited mast cell degranulation by inhibiting beta-hexosaminidase and histamine release, as well as the release of IL-4 and TNF-α at varying levels compared with ketotifen fumarate. Conclusion: The study suggested that M. oleifera and its isolated compounds potentially have an anti-allergic activity by inhibiting both early and late phases of allergic reactions.

Original languageEnglish
Article number361
JournalBMC complementary and alternative medicine
Volume19
Issue number1
DOIs
Publication statusPublished - 11 Dec 2019

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Moringa oleifera
Anti-Allergic Agents
Interleukin-4
Ketotifen
beta-N-Acetylhexosaminidases
Histamine Release
Hypersensitivity
Stigmasterol
Cell Degranulation
Electrospray Ionization Mass Spectrometry
Cotyledon
Herbal Medicine
Quercetin
Glucosides
Polyphenols
Oleic Acid
Mast Cells
Leukemia
Ethanol
Magnetic Resonance Spectroscopy

Keywords

  • Anti-allergic
  • Beta-hexosaminidase
  • Histamine
  • IL-4
  • Isolation
  • Moringa oleifera
  • New compound
  • RBL-2H3
  • TNF- α

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

In vitro anti-allergic activity of Moringa oleifera Lam. extracts and their isolated compounds. / Abd Rani, Nur Zahirah; Kumolosasi, Endang; Jasamai, Malina; Jamal, Jamia Azdina; Lam, Kok Wai; Husain, Khairana.

In: BMC complementary and alternative medicine, Vol. 19, No. 1, 361, 11.12.2019.

Research output: Contribution to journalArticle

Abd Rani, Nur Zahirah ; Kumolosasi, Endang ; Jasamai, Malina ; Jamal, Jamia Azdina ; Lam, Kok Wai ; Husain, Khairana. / In vitro anti-allergic activity of Moringa oleifera Lam. extracts and their isolated compounds. In: BMC complementary and alternative medicine. 2019 ; Vol. 19, No. 1.
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AU - Jamal, Jamia Azdina

AU - Lam, Kok Wai

AU - Husain, Khairana

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