In vitro and in vivo effects of three different mitragyna speciosa korth leaf extracts on phase II drug metabolizing enzymes-glutathione transferases (GSTs)

Juzaili Azizi, Sabariah Ismail, Mohd Nizam Mordi, Surash Ramanathan, Mohd Ikram Mohd Said, Sharif Mahsufi Mansor

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    In the present study, we investigate the effects of three different Mitragyna speciosa extracts, namely methanolic, aqueous and total alkaloid extracts, on glutathione transferase-specific activity in male Sprague Dawley rat liver cytosol in vitro and in vivo. In the in vitro study, the effect of Mitragyna speciosa extracts (0.01 to 750 μg/mL) against the specific activity of glutathione transferases was examined in rat liver cytosolic fraction from untreated rats. Our data show concentration dependent inhibition of cytosolic GSTs when Mitragyna speciosa extract was added into the reaction mixture. At the highest concentration used, the methanolic extract showed the highest GSTs specific activity inhibition (61%), followed by aqueous (50%) and total alkaloid extract (43%), respectively. In in vivo study, three different dosages; 50, 100 and 200 mg/kg for methanolic and aqueous extracts and 5, 10 and 20 mg/kg for total alkaloid extract were given orally for 14 days. An increase in GST specific activity was generally observed. However, only Mitragyna speciosa aqueous extract with a dosage of 100 mg/kg showed significant results: 129%) compared to control.

    Original languageEnglish
    Pages (from-to)432-441
    Number of pages10
    JournalMolecules
    Volume15
    Issue number1
    DOIs
    Publication statusPublished - Jan 2010

    Fingerprint

    Mitragyna
    glutathione
    Glutathione Transferase
    Alkaloids
    leaves
    alkaloids
    enzymes
    Rats
    drugs
    rats
    Liver
    Enzymes
    liver
    Pharmaceutical Preparations
    dosage
    Cytosol
    Sprague Dawley Rats
    In Vitro Techniques

    Keywords

    • Glutathione transferases
    • Herb-drug interactions
    • In vitro
    • In vivo
    • Mitragyna speciosa

    ASJC Scopus subject areas

    • Organic Chemistry

    Cite this

    In vitro and in vivo effects of three different mitragyna speciosa korth leaf extracts on phase II drug metabolizing enzymes-glutathione transferases (GSTs). / Azizi, Juzaili; Ismail, Sabariah; Mordi, Mohd Nizam; Ramanathan, Surash; Said, Mohd Ikram Mohd; Mansor, Sharif Mahsufi.

    In: Molecules, Vol. 15, No. 1, 01.2010, p. 432-441.

    Research output: Contribution to journalArticle

    Azizi, Juzaili ; Ismail, Sabariah ; Mordi, Mohd Nizam ; Ramanathan, Surash ; Said, Mohd Ikram Mohd ; Mansor, Sharif Mahsufi. / In vitro and in vivo effects of three different mitragyna speciosa korth leaf extracts on phase II drug metabolizing enzymes-glutathione transferases (GSTs). In: Molecules. 2010 ; Vol. 15, No. 1. pp. 432-441.
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