In vitro and in vivo antitumour effects of coconut water vinegar on 4T1 breast cancer cells

Nurul Elyani Mohamad, Swee Keong Yeap, Nadiah Abu, Kian Lam Lim, Nur Rizi Zamberi, Noraini Nordin, Shaiful Adzni Sharifuddin, Kamariah Long, Noorjahan Banu Alitheen

Research output: Contribution to journalArticle

Abstract

Background: Coconut water and vinegars have been reported to possess potential anti-tumour and immunostimulatory effects. However, the anti-tumour, anti-inflammatory and immunostimulatory effects of coconut water vinegar have yet to be tested. Objective: This study investigated the in vitro and in vivo anti-tumour effects of coconut water vinegar on 4T1 breast cancer cells. Methods: The 4T1 cells were treated with freeze-dried coconut water vinegar and subjected to MTT cell viability, BrdU, annexin V/PI apoptosis, cell cycle and wound healing assays for the in vitro analysis. For the in vivo chemopreventive evaluation, mice challenged with 4T1 cells were treated with 0.08or 2.00 mL/kg body weight of fresh coconut water vinegar for 28 days. Tumour weight, apoptosis of tumour cells, metastasis and immunity of untreated mice and coconut water vinegar-treated 4T1 challenged mice were compared. Results: Freeze-dried coconut water vinegar reduced the cell viability, induced apoptosis and delayed the wound healing effect of 4T1 cells in vitro. In vivo, coconut water vinegar delayed 4T1 breast cancer progression in mice by inducing apoptosis and delaying the metastasis. Furthermore, coconut water vinegar also promoted immune cell cytotoxicity and production of anticancer cytokines. The results indicate that coconut water vinegar delays breast cancer progression by inducing apoptosis in breast cancer cells, suppressing metastasis and activating anti-tumour immunity. Conclusion: Coconut water vinegar is a potential health food ingredient with a chemopreventive effect.

Original languageEnglish
Article number1616
JournalFood and Nutrition Research
Volume63
DOIs
Publication statusPublished - 1 Jan 2019
Externally publishedYes

Fingerprint

Cocos
vinegars
Acetic Acid
breast neoplasms
Breast Neoplasms
Water
apoptosis
Apoptosis
desiccated coconut
neoplasms
metastasis
mice
Neoplasms
Neoplasm Metastasis
tissue repair
Wound Healing
cell viability
Immunity
Cell Survival
In Vitro Techniques

Keywords

  • Breast cancer
  • Coconut water vinegar
  • Metastasis

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics
  • Public Health, Environmental and Occupational Health

Cite this

Mohamad, N. E., Yeap, S. K., Abu, N., Lim, K. L., Zamberi, N. R., Nordin, N., ... Alitheen, N. B. (2019). In vitro and in vivo antitumour effects of coconut water vinegar on 4T1 breast cancer cells. Food and Nutrition Research, 63, [1616]. https://doi.org/10.29219/fnr.v63.1616

In vitro and in vivo antitumour effects of coconut water vinegar on 4T1 breast cancer cells. / Mohamad, Nurul Elyani; Yeap, Swee Keong; Abu, Nadiah; Lim, Kian Lam; Zamberi, Nur Rizi; Nordin, Noraini; Sharifuddin, Shaiful Adzni; Long, Kamariah; Alitheen, Noorjahan Banu.

In: Food and Nutrition Research, Vol. 63, 1616, 01.01.2019.

Research output: Contribution to journalArticle

Mohamad, NE, Yeap, SK, Abu, N, Lim, KL, Zamberi, NR, Nordin, N, Sharifuddin, SA, Long, K & Alitheen, NB 2019, 'In vitro and in vivo antitumour effects of coconut water vinegar on 4T1 breast cancer cells', Food and Nutrition Research, vol. 63, 1616. https://doi.org/10.29219/fnr.v63.1616
Mohamad, Nurul Elyani ; Yeap, Swee Keong ; Abu, Nadiah ; Lim, Kian Lam ; Zamberi, Nur Rizi ; Nordin, Noraini ; Sharifuddin, Shaiful Adzni ; Long, Kamariah ; Alitheen, Noorjahan Banu. / In vitro and in vivo antitumour effects of coconut water vinegar on 4T1 breast cancer cells. In: Food and Nutrition Research. 2019 ; Vol. 63.
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