In silico identification of resistance and defense related genes for Bacterial Leaf Blight (BLB) in rice

Ilakiya Sharanee Kumar, Nadiah Zaharin, Kalaivani K. Nadarajah

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Bacterial leaf blight (BLB) disease is a rice disease caused by Xanthomonas oryzae pv.oryzae (Xoo). This disease causes devastating losses in the rice industry. To date research has been directed towards identifying QTLs and disease resistance genes that will afford resistance against Xoo. The rice plantations in different parts of the world are attacked by various strains of Xoo and thence the varieties that exhibit resistance to these strains may vary from one geographical location to another. In this paper we have analysed a QTL that has been recurrent in various rice backgrounds making it one of the three main QTLs responsible for BLB resistance in rice. qBBR11-1, a QTL found in chromosome eleven of rice is a good candidate for defense and resistance analysis towards BLB due to the various reports on the presence of multiple resistance genes in this QTL. Through the utilisation of bioinformatics tools we (1) identified and classified defense genes to functional groups, (2) identified and classified resistance genes according to the relevant domains, and finally, (3) mapped the relationship between defense related and resistance genes to build a defense model against BLB. A total of 21 defense genes have been classified according to functional groups such as chitinase activity, transduction signal and response to stress. A total of 37 putative resistance genes have been found and classified according to related domains such as nucleotide binding sites (NBS), trans membrane domains (Trd), WRKY domains and Ser/Thr Kin without LRR. Collectively all this information is used to provide a graphical representation of the interaction between the dense and the resistance gene systems. The key genes identified may be developed into markers for breeding or used in plant transformation works.

Original languageEnglish
Pages (from-to)1867-1876
Number of pages10
JournalJournal of Pure and Applied Microbiology
Volume12
Issue number4
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

Bacterial Genes
Computer Simulation
Genes
Beriberi
Xanthomonas
Oryza
Chitinases
Disease Resistance
Computational Biology
Breeding
Signal Transduction
Industry
Nucleotides
Chromosomes
Binding Sites
Membranes

Keywords

  • Bacterial leaf blight
  • Defense, and pathways
  • Dominant
  • Quantitative trait loci
  • Resistance

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

In silico identification of resistance and defense related genes for Bacterial Leaf Blight (BLB) in rice. / Kumar, Ilakiya Sharanee; Zaharin, Nadiah; K. Nadarajah, Kalaivani.

In: Journal of Pure and Applied Microbiology, Vol. 12, No. 4, 01.12.2018, p. 1867-1876.

Research output: Contribution to journalArticle

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