In silico identification and characterization of a putative phosphatidylinositol 4-phosphate 5-kinase (PIP5K) gene in Eimeria tenella

King Hwa Ling, Shu San Loo, Rozita Rosli, Mariana Nor Shamsudin, Rahmah Mohamed, Kiew Lian Wan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly. Discovery of the PIP5K gene in Eimeria tenella may shed light on its role in the biology of this avian protozoan, and afford further understanding of the cell-host interaction, particularly during the invasion process. In this study, we report the identification of the PIP5K coding region in the genome sequence of Eimeria tenella using in silico gene prediction approaches. Prediction of the PIP5K coding sequence was confirmed by mapping the full-length cDNA sequence, generated via the Rapid Amplification of cDNA Ends (RACE) method, to the genomic sequence. The putative PIP5K gene of Eimeria tenella is located on the complementary strand of the E1080B12.b1 contig, and comprises 12 exons. Further analysis showed that the coding region spans from exon 1 to exon 7, with all exons obeying the adopted 'gt...ag' splicing rule of intronic sequences. Consensus of the hexameric 5′ donor-splice site was deduced as GTRDBB... and the consensus for the 3′ acceptor-splice sites as... BHDYAG. The gene encodes a 252-amino acid residue protein. Domain search and protein fold recognition analyses provide compelling evidences that the deduced protein is a PIP5K.

Original languageEnglish
Pages (from-to)115-121
Number of pages7
JournalIn Silico Biology
Volume7
Issue number1
Publication statusPublished - 2007

Fingerprint

Eimeria tenella
Phosphate
Computer Simulation
Phosphates
Genes
Gene
Exons
RNA Splice Sites
Coding
CDNA
Proteins
Protein
Biology
Complementary DNA
Cytology
Cytoskeleton
Signal transduction
Signal Transduction
Prediction
Secretion

Keywords

  • Eimeria tenella
  • Homology-based approach
  • Intron-exon splicing
  • Phosphatidylinositol 4-phosphate 5-kinase
  • Protein fold recognition
  • Threading

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

In silico identification and characterization of a putative phosphatidylinositol 4-phosphate 5-kinase (PIP5K) gene in Eimeria tenella. / Ling, King Hwa; Loo, Shu San; Rosli, Rozita; Shamsudin, Mariana Nor; Mohamed, Rahmah; Wan, Kiew Lian.

In: In Silico Biology, Vol. 7, No. 1, 2007, p. 115-121.

Research output: Contribution to journalArticle

Ling, King Hwa ; Loo, Shu San ; Rosli, Rozita ; Shamsudin, Mariana Nor ; Mohamed, Rahmah ; Wan, Kiew Lian. / In silico identification and characterization of a putative phosphatidylinositol 4-phosphate 5-kinase (PIP5K) gene in Eimeria tenella. In: In Silico Biology. 2007 ; Vol. 7, No. 1. pp. 115-121.
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