In silico evaluation of miRNA binding site in mutated 3′UTR mRNA of G6PD

Syarifah Anis Wafa Binti Syed Mohd Azmi, Mohd Shihabudin Noorden, Nurul Yuziana Mohd Yusof, Endom Ismail

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

MicroRNAs (miRNAs) are small non coding RNA sized 21-25 nucleotide. It has the ability to bind to the 3′- untranslated regions (3′UTR) of their target genes. Consequently, the binding of miRNA in the 3′UTR of targeted mRNA will regulate the expression of this gene. Thus, changes in 3′UTR may affect miRNA binding to mRNA of their target gene, leading to aberrations in mRNA regulations or expression and likely contribute to the various phenotypic changes or clinical risk for certain diseases in man. Therefore, the aim of this study is to evaluate candidate miRNAs species involved during the regulation of glucose-6-phosphate dehydrogenase (G6PD) mRNA with and without a specific 3′UTR nucleotide change that was previously shown to be responsible for G6PD deficiency in a Negrito sub-group of the Malaysian Orang Asli. We have conducted in silico analysis using TargetScan, PITA, RegRNA 2.0 and miRanda platform. Our results indicate that three potential miRNAs may have a functional role towards the regulated expression of those bearing the 3′UTR mutation. The role of these eleven miRNA can be investigated in future in vitro expression studies in order to verify its miRNA:mRNA relationship.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
PublisherAmerican Institute of Physics Inc.
Volume1678
ISBN (Print)9780735413252
DOIs
Publication statusPublished - 25 Sep 2015
Event2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015 - Selangor, Malaysia
Duration: 15 Apr 201516 Apr 2015

Other

Other2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015
CountryMalaysia
CitySelangor
Period15/4/1516/4/15

Fingerprint

dehydrogenases
glucose
phosphates
genes
evaluation
nucleotides
Miranda
mutations
aberration
coding
platforms

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Azmi, S. A. W. B. S. M., Noorden, M. S., Mohd Yusof, N. Y., & Ismail, E. (2015). In silico evaluation of miRNA binding site in mutated 3′UTR mRNA of G6PD. In AIP Conference Proceedings (Vol. 1678). [030024] American Institute of Physics Inc.. https://doi.org/10.1063/1.4931245

In silico evaluation of miRNA binding site in mutated 3′UTR mRNA of G6PD. / Azmi, Syarifah Anis Wafa Binti Syed Mohd; Noorden, Mohd Shihabudin; Mohd Yusof, Nurul Yuziana; Ismail, Endom.

AIP Conference Proceedings. Vol. 1678 American Institute of Physics Inc., 2015. 030024.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Azmi, SAWBSM, Noorden, MS, Mohd Yusof, NY & Ismail, E 2015, In silico evaluation of miRNA binding site in mutated 3′UTR mRNA of G6PD. in AIP Conference Proceedings. vol. 1678, 030024, American Institute of Physics Inc., 2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015, Selangor, Malaysia, 15/4/15. https://doi.org/10.1063/1.4931245
Azmi SAWBSM, Noorden MS, Mohd Yusof NY, Ismail E. In silico evaluation of miRNA binding site in mutated 3′UTR mRNA of G6PD. In AIP Conference Proceedings. Vol. 1678. American Institute of Physics Inc. 2015. 030024 https://doi.org/10.1063/1.4931245
Azmi, Syarifah Anis Wafa Binti Syed Mohd ; Noorden, Mohd Shihabudin ; Mohd Yusof, Nurul Yuziana ; Ismail, Endom. / In silico evaluation of miRNA binding site in mutated 3′UTR mRNA of G6PD. AIP Conference Proceedings. Vol. 1678 American Institute of Physics Inc., 2015.
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