In silico analysis of subtilisin from Glaciozyma antarctica PI12

Siti Mardhiah Mustafha, Abdul Munir Abd. Murad, Nor Muhammad Mahadi, Shazilah Kamaruddin, Farah Diba Abu Bakar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Subtilisin constitute as a major player in industrial enzymes that has a wide range of application especially in the detergent industry. In this study, a cDNA encoding for subtilisin (GaSUBT) was extracted from the psychrophilic yeast, Glaciozyma antarctica PI12, PCR amplified and sequenced. Various bioinformatics tools were used to characterize the GaSUBT. GaSUBT contains 1587 bp nucleotides encoding for 529 amino acids. The predicted molecular weight of the deduced protein is 55.34 kDa with an isoelectric point of 6.25. GaSUBT was predicted to possess a signal peptide and pro-peptide consisting of a peptidase inhibitor I9 sequence. From the sequence alignment analysis of deduced amino acids with other subtilisins in the NCBI database showed that the sequences surrounding the catalytic triad that forms the catalytic domain are well conserved.

Original languageEnglish
Title of host publication2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1678
ISBN (Electronic)9780735413252
DOIs
Publication statusPublished - 25 Sep 2015
Event2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015 - Selangor, Malaysia
Duration: 15 Apr 201516 Apr 2015

Other

Other2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015
CountryMalaysia
CitySelangor
Period15/4/1516/4/15

Fingerprint

Antarctic regions
peptides
amino acids
coding
detergents
yeast
nucleotides
inhibitors
enzymes
molecular weight
industries
alignment
proteins

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Mustafha, S. M., Abd. Murad, A. M., Mahadi, N. M., Kamaruddin, S., & Abu Bakar, F. D. (2015). In silico analysis of subtilisin from Glaciozyma antarctica PI12. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium (Vol. 1678). [030025] American Institute of Physics Inc.. https://doi.org/10.1063/1.4931246

In silico analysis of subtilisin from Glaciozyma antarctica PI12. / Mustafha, Siti Mardhiah; Abd. Murad, Abdul Munir; Mahadi, Nor Muhammad; Kamaruddin, Shazilah; Abu Bakar, Farah Diba.

2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015. 030025.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mustafha, SM, Abd. Murad, AM, Mahadi, NM, Kamaruddin, S & Abu Bakar, FD 2015, In silico analysis of subtilisin from Glaciozyma antarctica PI12. in 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. vol. 1678, 030025, American Institute of Physics Inc., 2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015, Selangor, Malaysia, 15/4/15. https://doi.org/10.1063/1.4931246
Mustafha SM, Abd. Murad AM, Mahadi NM, Kamaruddin S, Abu Bakar FD. In silico analysis of subtilisin from Glaciozyma antarctica PI12. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678. American Institute of Physics Inc. 2015. 030025 https://doi.org/10.1063/1.4931246
Mustafha, Siti Mardhiah ; Abd. Murad, Abdul Munir ; Mahadi, Nor Muhammad ; Kamaruddin, Shazilah ; Abu Bakar, Farah Diba. / In silico analysis of subtilisin from Glaciozyma antarctica PI12. 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015.
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