In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia

Wong Kon Ken, Md. Mostafizur Rahman, Alfizah Hanafiah, Ilina Isahak

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives: Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children worldwide. Its detection by viral culture and immunofluorescent assay is time consuming and has low sensitivity. The objective of the study was to develop In House Light Cycler Real Time Polymerase Chain Reaction for Rapid Detection of Respiratory Syncytial Virus. Methodology: In house developed Light Cycler Real-Time reverse transcriptase polymerase chain reaction (LC-RT-PCR) assay was standardized using designed primers for the rapid identification of clinical samples of RSV. Results: A total of 130 samples (throat swab and nasopharyngeal aspirate) obtained from University Kebangsaan Malaysia Medical Centre, were efficiently tested and of which 54 (42%) were RSV positive. Conclusion: This study indicated that this newly developed in house rRT-PCR assay proved to be an excellent method for the rapid detection of RSV.

Original languageEnglish
Pages (from-to)818-822
Number of pages5
JournalPakistan Journal of Medical Sciences
Volume28
Issue number5
Publication statusPublished - Oct 2012

Fingerprint

Respiratory Syncytial Viruses
Malaysia
Real-Time Polymerase Chain Reaction
Light
Pharynx
Reverse Transcriptase Polymerase Chain Reaction
Polymerase Chain Reaction

Keywords

  • LightCycler real time polymerase chain reaction
  • Primer
  • Probe and Plasmid
  • Respiratory syncytial virus
  • Sequence

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia",
abstract = "Objectives: Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children worldwide. Its detection by viral culture and immunofluorescent assay is time consuming and has low sensitivity. The objective of the study was to develop In House Light Cycler Real Time Polymerase Chain Reaction for Rapid Detection of Respiratory Syncytial Virus. Methodology: In house developed Light Cycler Real-Time reverse transcriptase polymerase chain reaction (LC-RT-PCR) assay was standardized using designed primers for the rapid identification of clinical samples of RSV. Results: A total of 130 samples (throat swab and nasopharyngeal aspirate) obtained from University Kebangsaan Malaysia Medical Centre, were efficiently tested and of which 54 (42{\%}) were RSV positive. Conclusion: This study indicated that this newly developed in house rRT-PCR assay proved to be an excellent method for the rapid detection of RSV.",
keywords = "LightCycler real time polymerase chain reaction, Primer, Probe and Plasmid, Respiratory syncytial virus, Sequence",
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T1 - In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia

AU - Kon Ken, Wong

AU - Rahman, Md. Mostafizur

AU - Hanafiah, Alfizah

AU - Isahak, Ilina

PY - 2012/10

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N2 - Objectives: Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children worldwide. Its detection by viral culture and immunofluorescent assay is time consuming and has low sensitivity. The objective of the study was to develop In House Light Cycler Real Time Polymerase Chain Reaction for Rapid Detection of Respiratory Syncytial Virus. Methodology: In house developed Light Cycler Real-Time reverse transcriptase polymerase chain reaction (LC-RT-PCR) assay was standardized using designed primers for the rapid identification of clinical samples of RSV. Results: A total of 130 samples (throat swab and nasopharyngeal aspirate) obtained from University Kebangsaan Malaysia Medical Centre, were efficiently tested and of which 54 (42%) were RSV positive. Conclusion: This study indicated that this newly developed in house rRT-PCR assay proved to be an excellent method for the rapid detection of RSV.

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KW - Primer

KW - Probe and Plasmid

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KW - Sequence

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