Improving the methods for isolation of monocyte and establishing macrophage cell culture in caprine model

Nasruddin Nurrul Shaqinah, Mazlan Mazlina, M. Zamri-Saad, Hamzah Hazilawati, Sabri Jasni

Research output: Contribution to journalArticle

Abstract

Monocytes are widely used for immunological research, especially in the study of innate immune system. Although methods for isolation of human monocytes have been established, the procedure for non-human monocyte has not been well developed. This paper describes an improved method for isolation of monocyte and the subsequent macrophage cultivation from caprine blood. Monocytes were isolated from 16 ml of heparinized caprine blood using double density methods; the Ficoll and Percoll. The number of monocytes obtained was 5.12 ± 0.89 × 107 cells/ml at 70 % purity. The isolated monocytes were maintained in 10 % fetal bovine serum-enriched Dulbecco’s Modified Eagle Medium for maturation to form macrophage cell culture. At the end of the experiment, the harvested macrophage was 2.48 ± 0.33 × 106 cells/ml.

Original languageEnglish
Pages (from-to)1655-1659
Number of pages5
JournalCytotechnology
Volume68
Issue number4
DOIs
Publication statusPublished - 1 Aug 2016
Externally publishedYes

Fingerprint

Macrophages
Cell culture
Monocytes
Cell Culture Techniques
Blood
Ficoll
Immune system
Eagles
Experiments
Immune System
Serum
Research

Keywords

  • Caprine
  • Cell line
  • Isolation
  • Macrophage
  • Monocyte

ASJC Scopus subject areas

  • Cell Biology
  • Biotechnology
  • Biomedical Engineering
  • Bioengineering
  • Clinical Biochemistry

Cite this

Improving the methods for isolation of monocyte and establishing macrophage cell culture in caprine model. / Nurrul Shaqinah, Nasruddin; Mazlina, Mazlan; Zamri-Saad, M.; Hazilawati, Hamzah; Jasni, Sabri.

In: Cytotechnology, Vol. 68, No. 4, 01.08.2016, p. 1655-1659.

Research output: Contribution to journalArticle

Nurrul Shaqinah, Nasruddin ; Mazlina, Mazlan ; Zamri-Saad, M. ; Hazilawati, Hamzah ; Jasni, Sabri. / Improving the methods for isolation of monocyte and establishing macrophage cell culture in caprine model. In: Cytotechnology. 2016 ; Vol. 68, No. 4. pp. 1655-1659.
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