Improvement of the butanol production selectivity and butanol to acetone ratio (B: A) by addition of electron carriers in the batch culture of a new local isolate of Clostridium acetobutylicum YM1

Najeeb Kaid Nasser Al-Shorgani, Mohd. Sahaid Kalil, Wan Mohtar Wan Yusoff, Hafiza Shukor, Aidil Abdul Hamid

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9 Citations (Scopus)

Abstract

Improvement in the butanol production selectivity or enhanced butanol:acetone ratio (B:A) is desirable in acetone-butanol-ethanol (ABE) fermentation by Clostridium strains. In this study, artificial electron carriers were added to the fermentation medium of a new isolate of Clostridium acetobutylicum YM1 in order to improve the butanol yield and B:A ratio. The results revealed that medium supplementation with electron carriers changed the metabolism flux of electron and carbon in ABE fermentation by YM1. A decrease in acetone production, which subsequently improved the B:A ratio, was observed. Further improvement in the butanol production and B:A ratios were obtained when the fermentation medium was supplemented with butyric acid. The maximum butanol production (18.20 ± 1.38 g/L) was gained when a combination of methyl red and butyric acid was added. Although the addition of benzyl viologen (0.1 mM) and butyric acid resulted in high a B:A ratio of 16:1 (800% increment compared with the conventional 2:1 ratio), the addition of benzyl viologen to the culture after 4 h resulted in the production of 18.05 g/L butanol. Manipulating the metabolic flux to butanol through the addition of electron carriers could become an alternative strategy to achieve higher butanol productivity and improve the B:A ratio.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalAnaerobe
Volume36
DOIs
Publication statusPublished - 1 Dec 2015

Fingerprint

Clostridium acetobutylicum
Butanols
Batch Cell Culture Techniques
Acetone
Electrons
Fermentation
Butyric Acid
Benzyl Viologen
Ethanol
Carbon Cycle
Clostridium

Keywords

  • B:A ratio
  • Butanol
  • Clostridium acetobutylicum YM1
  • Electron carriers
  • Renewable energy

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

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title = "Improvement of the butanol production selectivity and butanol to acetone ratio (B: A) by addition of electron carriers in the batch culture of a new local isolate of Clostridium acetobutylicum YM1",
abstract = "Improvement in the butanol production selectivity or enhanced butanol:acetone ratio (B:A) is desirable in acetone-butanol-ethanol (ABE) fermentation by Clostridium strains. In this study, artificial electron carriers were added to the fermentation medium of a new isolate of Clostridium acetobutylicum YM1 in order to improve the butanol yield and B:A ratio. The results revealed that medium supplementation with electron carriers changed the metabolism flux of electron and carbon in ABE fermentation by YM1. A decrease in acetone production, which subsequently improved the B:A ratio, was observed. Further improvement in the butanol production and B:A ratios were obtained when the fermentation medium was supplemented with butyric acid. The maximum butanol production (18.20 ± 1.38 g/L) was gained when a combination of methyl red and butyric acid was added. Although the addition of benzyl viologen (0.1 mM) and butyric acid resulted in high a B:A ratio of 16:1 (800{\%} increment compared with the conventional 2:1 ratio), the addition of benzyl viologen to the culture after 4 h resulted in the production of 18.05 g/L butanol. Manipulating the metabolic flux to butanol through the addition of electron carriers could become an alternative strategy to achieve higher butanol productivity and improve the B:A ratio.",
keywords = "B:A ratio, Butanol, Clostridium acetobutylicum YM1, Electron carriers, Renewable energy",
author = "{Nasser Al-Shorgani}, {Najeeb Kaid} and Kalil, {Mohd. Sahaid} and {Wan Yusoff}, {Wan Mohtar} and Hafiza Shukor and {Abdul Hamid}, Aidil",
year = "2015",
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T1 - Improvement of the butanol production selectivity and butanol to acetone ratio (B

T2 - A) by addition of electron carriers in the batch culture of a new local isolate of Clostridium acetobutylicum YM1

AU - Nasser Al-Shorgani, Najeeb Kaid

AU - Kalil, Mohd. Sahaid

AU - Wan Yusoff, Wan Mohtar

AU - Shukor, Hafiza

AU - Abdul Hamid, Aidil

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Improvement in the butanol production selectivity or enhanced butanol:acetone ratio (B:A) is desirable in acetone-butanol-ethanol (ABE) fermentation by Clostridium strains. In this study, artificial electron carriers were added to the fermentation medium of a new isolate of Clostridium acetobutylicum YM1 in order to improve the butanol yield and B:A ratio. The results revealed that medium supplementation with electron carriers changed the metabolism flux of electron and carbon in ABE fermentation by YM1. A decrease in acetone production, which subsequently improved the B:A ratio, was observed. Further improvement in the butanol production and B:A ratios were obtained when the fermentation medium was supplemented with butyric acid. The maximum butanol production (18.20 ± 1.38 g/L) was gained when a combination of methyl red and butyric acid was added. Although the addition of benzyl viologen (0.1 mM) and butyric acid resulted in high a B:A ratio of 16:1 (800% increment compared with the conventional 2:1 ratio), the addition of benzyl viologen to the culture after 4 h resulted in the production of 18.05 g/L butanol. Manipulating the metabolic flux to butanol through the addition of electron carriers could become an alternative strategy to achieve higher butanol productivity and improve the B:A ratio.

AB - Improvement in the butanol production selectivity or enhanced butanol:acetone ratio (B:A) is desirable in acetone-butanol-ethanol (ABE) fermentation by Clostridium strains. In this study, artificial electron carriers were added to the fermentation medium of a new isolate of Clostridium acetobutylicum YM1 in order to improve the butanol yield and B:A ratio. The results revealed that medium supplementation with electron carriers changed the metabolism flux of electron and carbon in ABE fermentation by YM1. A decrease in acetone production, which subsequently improved the B:A ratio, was observed. Further improvement in the butanol production and B:A ratios were obtained when the fermentation medium was supplemented with butyric acid. The maximum butanol production (18.20 ± 1.38 g/L) was gained when a combination of methyl red and butyric acid was added. Although the addition of benzyl viologen (0.1 mM) and butyric acid resulted in high a B:A ratio of 16:1 (800% increment compared with the conventional 2:1 ratio), the addition of benzyl viologen to the culture after 4 h resulted in the production of 18.05 g/L butanol. Manipulating the metabolic flux to butanol through the addition of electron carriers could become an alternative strategy to achieve higher butanol productivity and improve the B:A ratio.

KW - B:A ratio

KW - Butanol

KW - Clostridium acetobutylicum YM1

KW - Electron carriers

KW - Renewable energy

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