Improvement of excretory overexpression for Bacillus sp. G1 cyclodextrin glucanotransferase (CGTase) in recombinant Escherichia coli through medium optimization

P. K. Lo, C. Y. Tan, Osman Hassan, A. Ahmad, N. M. Mahadi, R. M. Illias

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the study presented, Design of Experiments (DOE) was combined with statistical analysis such as fractional factorial design and small central composite design Response Surface Methodology (RSM) to significantly increase the extracelullar recombinant CGTase yields in fermentation flasks. The new medium obtained by the statistical analysis for the significant medium components comprised of 12 g L-1 NZ Amine A., 24 g L-1 yeast extract, 9.44 g L-1 KH2PO4, 4.4 g L -1 K2HPO4, 4.58 mLL-1 glycerol, 7 mg L-1 sucrose and 3 mg L-1 CuCl2. Yields were improved about 68% from 9.54 to 16.07 kU mL-1 in flasks when using the optimized cultivation medium. The results suggest that the overexpression level of recombinant CGTase excreted into the culture medium using the recombinant Escherichia coli could be improved through medium optimization.

Original languageEnglish
Pages (from-to)184-193
Number of pages10
JournalBiotechnology
Volume8
Issue number2
DOIs
Publication statusPublished - 2009

Fingerprint

Bacilli
Escherichia coli
Bacillus
Statistical methods
Yeast
Design of experiments
Glycerol
Fermentation
Amines
Culture Media
Sucrose
Yeasts
Composite materials
cyclomaltodextrin glucanotransferase
cupric chloride
potassium phosphate

Keywords

  • E. coli
  • Extracellular recombinant CGTase
  • Medium optimization
  • RSM

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Improvement of excretory overexpression for Bacillus sp. G1 cyclodextrin glucanotransferase (CGTase) in recombinant Escherichia coli through medium optimization. / Lo, P. K.; Tan, C. Y.; Hassan, Osman; Ahmad, A.; Mahadi, N. M.; Illias, R. M.

In: Biotechnology, Vol. 8, No. 2, 2009, p. 184-193.

Research output: Contribution to journalArticle

Lo, P. K. ; Tan, C. Y. ; Hassan, Osman ; Ahmad, A. ; Mahadi, N. M. ; Illias, R. M. / Improvement of excretory overexpression for Bacillus sp. G1 cyclodextrin glucanotransferase (CGTase) in recombinant Escherichia coli through medium optimization. In: Biotechnology. 2009 ; Vol. 8, No. 2. pp. 184-193.
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