Improved production of live cells of Lactobacillus rhamnosus by continuous cultivation using glucose-yeast extract medium

Siew Ling Liew, Rosfarizan Mohamad, Raha Abdul Rahim, Yin Wan Ho, Arbakariya Bin Ariff

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, the growth kinetics of Lactobacillus rhamnosus and lactic acid production in continuous culture were assessed at a range of dilution rates (0.05 h-1 to 0.40 h-1) using a 2 L stirred tank fermenter with a working volume of 600 ml. Unstructured models, predicated on the Monod and Luedeking-Piret equations, were employed to simulate the growth of the bacterium, glucose consumption, and lactic acid production at different dilution rates in continuous cultures. The maximum specific growth rate of L. rhamnosus, μmax, was estimated at 0.40 h-1, and the Monod cell growth saturation constant, Ks, at approximately 0.25 g/L. Maximum cell viability (1.3 × 1010 CFU/ml) was achieved in the dilution rate range of D = 0.28 h-1 to 0.35 h-1. Both maximum viable cell yield and productivity were achieved at D = 0.35 h-1. The continuous cultivation of L. rhamnosus at D = 0.35 h-1 resulted in substantial improvements in cell productivity, of 267% (viable cell count) that achieved via batch cultivation.

Original languageEnglish
Pages (from-to)439-446
Number of pages8
JournalJournal of Microbiology
Volume44
Issue number4
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Lactobacillus rhamnosus
Yeasts
Glucose
Growth
Lactic Acid
Cell Survival
Cell Count
Bacteria

Keywords

  • Continuous culture
  • Lactic acid
  • Lactobacillus rhamnosus
  • Modeling
  • Probiotic

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Improved production of live cells of Lactobacillus rhamnosus by continuous cultivation using glucose-yeast extract medium. / Liew, Siew Ling; Mohamad, Rosfarizan; Rahim, Raha Abdul; Ho, Yin Wan; Ariff, Arbakariya Bin.

In: Journal of Microbiology, Vol. 44, No. 4, 2006, p. 439-446.

Research output: Contribution to journalArticle

Liew, Siew Ling ; Mohamad, Rosfarizan ; Rahim, Raha Abdul ; Ho, Yin Wan ; Ariff, Arbakariya Bin. / Improved production of live cells of Lactobacillus rhamnosus by continuous cultivation using glucose-yeast extract medium. In: Journal of Microbiology. 2006 ; Vol. 44, No. 4. pp. 439-446.
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