Improved prediction for medium optimization using factorial screening for docosahexaenoic acid production by Schizochytrium SP. SW1

Vidyah Manikan, Mohd. Sahaid Kalil, Mohd Hafez Mohd Isa, Aidil Abdul Hamid

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Docosa Hexaenoic Acid (DHA) is an essential fatty acid that plays major role in human health. It is typically extracted from fatty fish and this puts pressure on global fish stock. DHA obtained from fish is also prone to contamination. Schizochytrium is a potential alternative source, having the ability of accumulating considerable amounts of DHA. Modification of medium components and culture conditions are the preferred techniques to enhance DHA production by this marine microalga. In this study, two-level factorial design was employed to investigate the significance of sea salt, glucose, yeast extract, Monosodium Glutamate (MSG) and peptone in affecting the biomass, lipid and DHA accumulation processes of a locally isolated strain of Schizochytrium. In contrast to the conventional ANOVA method commonly employed in various screening studies, half normal probability plots were used to identify the significant factors, whereas interaction plots were used to explain the pattern of interaction between significant combinations. Design Expert software was used to construct a set of experiments where each medium component mentioned above was varied over two levels. Cultivation was carried out in 250 mL flasks containing 50 mL of medium, incubated at 30°C with 200 rpm agitation for 96 h. This experiment revealed that sea salt and interaction of glucose-MSG have significant effect on biomass production whereas sea salt and interaction of sea salt-glucose affected lipid accumulation. Meanwhile, interactions of glucose-yeast extract and glucose-MSG have significant effect on DHA accumulation. Detailed analysis of interaction plots enabled deeper understanding of the behavior of the system in response to the significant variables, which improves the precision in determination of variable domains for effective medium optimization.

Original languageEnglish
Pages (from-to)462-474
Number of pages13
JournalAmerican Journal of Applied Sciences
Volume11
Issue number3
DOIs
Publication statusPublished - 15 Jan 2014

Fingerprint

sea salt
glucose
acid
prediction
yeast
lipid
microalga
biomass
fish
screening
fatty acid
experiment
software
effect

Keywords

  • Docosahexaenoic Acid
  • Factorial design
  • Schizochytrium
  • Screening of medium components

ASJC Scopus subject areas

  • General

Cite this

@article{58fd7988bc4242b99b5f07be4f7281fc,
title = "Improved prediction for medium optimization using factorial screening for docosahexaenoic acid production by Schizochytrium SP. SW1",
abstract = "Docosa Hexaenoic Acid (DHA) is an essential fatty acid that plays major role in human health. It is typically extracted from fatty fish and this puts pressure on global fish stock. DHA obtained from fish is also prone to contamination. Schizochytrium is a potential alternative source, having the ability of accumulating considerable amounts of DHA. Modification of medium components and culture conditions are the preferred techniques to enhance DHA production by this marine microalga. In this study, two-level factorial design was employed to investigate the significance of sea salt, glucose, yeast extract, Monosodium Glutamate (MSG) and peptone in affecting the biomass, lipid and DHA accumulation processes of a locally isolated strain of Schizochytrium. In contrast to the conventional ANOVA method commonly employed in various screening studies, half normal probability plots were used to identify the significant factors, whereas interaction plots were used to explain the pattern of interaction between significant combinations. Design Expert software was used to construct a set of experiments where each medium component mentioned above was varied over two levels. Cultivation was carried out in 250 mL flasks containing 50 mL of medium, incubated at 30°C with 200 rpm agitation for 96 h. This experiment revealed that sea salt and interaction of glucose-MSG have significant effect on biomass production whereas sea salt and interaction of sea salt-glucose affected lipid accumulation. Meanwhile, interactions of glucose-yeast extract and glucose-MSG have significant effect on DHA accumulation. Detailed analysis of interaction plots enabled deeper understanding of the behavior of the system in response to the significant variables, which improves the precision in determination of variable domains for effective medium optimization.",
keywords = "Docosahexaenoic Acid, Factorial design, Schizochytrium, Screening of medium components",
author = "Vidyah Manikan and Kalil, {Mohd. Sahaid} and {Mohd Isa}, {Mohd Hafez} and {Abdul Hamid}, Aidil",
year = "2014",
month = "1",
day = "15",
doi = "10.3844/ajassp.2014.462.474",
language = "English",
volume = "11",
pages = "462--474",
journal = "American Journal of Applied Sciences",
issn = "1546-9239",
publisher = "Science Publications",
number = "3",

}

TY - JOUR

T1 - Improved prediction for medium optimization using factorial screening for docosahexaenoic acid production by Schizochytrium SP. SW1

AU - Manikan, Vidyah

AU - Kalil, Mohd. Sahaid

AU - Mohd Isa, Mohd Hafez

AU - Abdul Hamid, Aidil

PY - 2014/1/15

Y1 - 2014/1/15

N2 - Docosa Hexaenoic Acid (DHA) is an essential fatty acid that plays major role in human health. It is typically extracted from fatty fish and this puts pressure on global fish stock. DHA obtained from fish is also prone to contamination. Schizochytrium is a potential alternative source, having the ability of accumulating considerable amounts of DHA. Modification of medium components and culture conditions are the preferred techniques to enhance DHA production by this marine microalga. In this study, two-level factorial design was employed to investigate the significance of sea salt, glucose, yeast extract, Monosodium Glutamate (MSG) and peptone in affecting the biomass, lipid and DHA accumulation processes of a locally isolated strain of Schizochytrium. In contrast to the conventional ANOVA method commonly employed in various screening studies, half normal probability plots were used to identify the significant factors, whereas interaction plots were used to explain the pattern of interaction between significant combinations. Design Expert software was used to construct a set of experiments where each medium component mentioned above was varied over two levels. Cultivation was carried out in 250 mL flasks containing 50 mL of medium, incubated at 30°C with 200 rpm agitation for 96 h. This experiment revealed that sea salt and interaction of glucose-MSG have significant effect on biomass production whereas sea salt and interaction of sea salt-glucose affected lipid accumulation. Meanwhile, interactions of glucose-yeast extract and glucose-MSG have significant effect on DHA accumulation. Detailed analysis of interaction plots enabled deeper understanding of the behavior of the system in response to the significant variables, which improves the precision in determination of variable domains for effective medium optimization.

AB - Docosa Hexaenoic Acid (DHA) is an essential fatty acid that plays major role in human health. It is typically extracted from fatty fish and this puts pressure on global fish stock. DHA obtained from fish is also prone to contamination. Schizochytrium is a potential alternative source, having the ability of accumulating considerable amounts of DHA. Modification of medium components and culture conditions are the preferred techniques to enhance DHA production by this marine microalga. In this study, two-level factorial design was employed to investigate the significance of sea salt, glucose, yeast extract, Monosodium Glutamate (MSG) and peptone in affecting the biomass, lipid and DHA accumulation processes of a locally isolated strain of Schizochytrium. In contrast to the conventional ANOVA method commonly employed in various screening studies, half normal probability plots were used to identify the significant factors, whereas interaction plots were used to explain the pattern of interaction between significant combinations. Design Expert software was used to construct a set of experiments where each medium component mentioned above was varied over two levels. Cultivation was carried out in 250 mL flasks containing 50 mL of medium, incubated at 30°C with 200 rpm agitation for 96 h. This experiment revealed that sea salt and interaction of glucose-MSG have significant effect on biomass production whereas sea salt and interaction of sea salt-glucose affected lipid accumulation. Meanwhile, interactions of glucose-yeast extract and glucose-MSG have significant effect on DHA accumulation. Detailed analysis of interaction plots enabled deeper understanding of the behavior of the system in response to the significant variables, which improves the precision in determination of variable domains for effective medium optimization.

KW - Docosahexaenoic Acid

KW - Factorial design

KW - Schizochytrium

KW - Screening of medium components

UR - http://www.scopus.com/inward/record.url?scp=84896467325&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896467325&partnerID=8YFLogxK

U2 - 10.3844/ajassp.2014.462.474

DO - 10.3844/ajassp.2014.462.474

M3 - Article

AN - SCOPUS:84896467325

VL - 11

SP - 462

EP - 474

JO - American Journal of Applied Sciences

JF - American Journal of Applied Sciences

SN - 1546-9239

IS - 3

ER -