Improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics

Mohd Hafis Yuswan, Wan Mohd Aizat Wan Kamaruddin, Mohd Nasir Mohd Desa, Amalia Mohd Hashim, Nur Ajreena Rahim, Shuhaimi Mustafa, Rozi Mohamed, Dhilia Udie Lamasudin

Research output: Contribution to journalArticle

Abstract

Numerous analytical methods for the authentication of halal meat are now well established, with gel-enhanced liquid chromatography-mass spectrometry (GeLCMS) being a popular approach. However, the selection of potential protein markers on 1-dimensional gel electrophoresis (1DE) prior to LCMS is considered problematic, because using the optical density for the selection process could introduce human error. In this study, an improved GeLCMS method assisted by multivariate principal component analysis (PCA) was developed to identify the potential protein markers for non-halal pork among halal beef and chicken. The improved GeLCMS technique allowed for the confident excising of identified protein bands prior to in-gel tryptic digestion. The inferential protein markers (myofibrillar proteins), which might be present in the samples, were determined based on the identified sequence of peptides. This chemometric-assisted GeLCMS could potentially be used as a guideline to assist chemists in analysis of any gel-based separation of biomolecules, regardless of the field of study.

Original languageEnglish
Article number103825
JournalChemometrics and Intelligent Laboratory Systems
Volume192
DOIs
Publication statusPublished - 15 Sep 2019

Fingerprint

Liquid chromatography
Mass spectrometry
Gels
Proteins
Beef
Density (optical)
Meats
Biomolecules
Proteomics
Electrophoresis
Principal component analysis
Authentication
Peptides

Keywords

  • Bottom-up proteomics
  • Chemometrics
  • GeLCMS
  • PCA
  • Protein marker

ASJC Scopus subject areas

  • Analytical Chemistry
  • Software
  • Computer Science Applications
  • Process Chemistry and Technology
  • Spectroscopy

Cite this

Improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics. / Yuswan, Mohd Hafis; Wan Kamaruddin, Wan Mohd Aizat; Desa, Mohd Nasir Mohd; Hashim, Amalia Mohd; Rahim, Nur Ajreena; Mustafa, Shuhaimi; Mohamed, Rozi; Lamasudin, Dhilia Udie.

In: Chemometrics and Intelligent Laboratory Systems, Vol. 192, 103825, 15.09.2019.

Research output: Contribution to journalArticle

Yuswan, Mohd Hafis ; Wan Kamaruddin, Wan Mohd Aizat ; Desa, Mohd Nasir Mohd ; Hashim, Amalia Mohd ; Rahim, Nur Ajreena ; Mustafa, Shuhaimi ; Mohamed, Rozi ; Lamasudin, Dhilia Udie. / Improved gel-enhanced liquid chromatography-mass spectrometry by chemometrics for halal proteomics. In: Chemometrics and Intelligent Laboratory Systems. 2019 ; Vol. 192.
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