Improved Fermentability of Pretreated Glycerol Enhanced Bioconversion of 1,3-Propanediol

Jian Ping Tan, Zhao Kang Tee, Wan Isahak Wan Nor Roslam, Byung Hong Kim, Ahmad Jaril Asis, Jamaliah Md Jahim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The potential of biodiesel-derived glycerol to be valorized into 1,3-propanediol (1,3-PDO) was hindered by the impurities contained therein. This study introduced a straightforward and effective pretreatment process through microfiltration to remove free fatty acids (FFAs) that could inhibit the fermentation which resulted in remarkable high productivity in batch fermentation. This approach appreciably reduces the cost of pretreatment as compared to previously reported acidification, solvent extraction, electrodialysis, activated carbon adsorption and ion-exchange resins. From the fermentation results, a high productivity of 1,3-PDO of 2.16 g L-1 h-1 was attained when 20 g/L pretreated glycerol was used as the carbon source, an improvement of 62.4% compared with fermentation with crude glycerol using locally isolated strain Clostridium butyricum JKT37, and among the highest compared to previous reports. These results suggested that, with a simple microfiltration pretreatment, the utility of the resultant biodiesel-derived glycerol in bioproduction of 1,3-PDO is comparable to that of pure glycerol. The physical and chemical properties of the biodiesel-derived glycerol were characterized in accordance with American Oil Chemists' Society (AOCS), International Organization for Standardization (ISO), and American Society for Testing and Materials (ASTM) standard methods, before and after the pretreatment. The sample of crude glycerol comprised 75 wt % glycerol, 15% soap, 8% moisture, 0.8% methanol, and less than 5% ash. FFAs generated from crude glycerol were removed completely by microfiltration.

Original languageEnglish
Pages (from-to)12565-12573
Number of pages9
JournalIndustrial and Engineering Chemistry Research
Volume57
Issue number37
DOIs
Publication statusPublished - 19 Sep 2018

Fingerprint

Bioconversion
Glycerol
Fermentation
Microfiltration
Biofuels
Biodiesel
Ashes
Fatty acids
Nonesterified Fatty Acids
Productivity
Ion Exchange Resins
1,3-propanediol
Electrodialysis
Clostridium
Ion exchange resins
Soaps (detergents)
Acidification
Solvent extraction
Activated carbon
Chemical properties

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Improved Fermentability of Pretreated Glycerol Enhanced Bioconversion of 1,3-Propanediol. / Tan, Jian Ping; Tee, Zhao Kang; Wan Nor Roslam, Wan Isahak; Kim, Byung Hong; Asis, Ahmad Jaril; Md Jahim, Jamaliah.

In: Industrial and Engineering Chemistry Research, Vol. 57, No. 37, 19.09.2018, p. 12565-12573.

Research output: Contribution to journalArticle

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