Abstract
The potential of biodiesel-derived glycerol to be valorized into 1,3-propanediol (1,3-PDO) was hindered by the impurities contained therein. This study introduced a straightforward and effective pretreatment process through microfiltration to remove free fatty acids (FFAs) that could inhibit the fermentation which resulted in remarkable high productivity in batch fermentation. This approach appreciably reduces the cost of pretreatment as compared to previously reported acidification, solvent extraction, electrodialysis, activated carbon adsorption and ion-exchange resins. From the fermentation results, a high productivity of 1,3-PDO of 2.16 g L-1 h-1 was attained when 20 g/L pretreated glycerol was used as the carbon source, an improvement of 62.4% compared with fermentation with crude glycerol using locally isolated strain Clostridium butyricum JKT37, and among the highest compared to previous reports. These results suggested that, with a simple microfiltration pretreatment, the utility of the resultant biodiesel-derived glycerol in bioproduction of 1,3-PDO is comparable to that of pure glycerol. The physical and chemical properties of the biodiesel-derived glycerol were characterized in accordance with American Oil Chemists' Society (AOCS), International Organization for Standardization (ISO), and American Society for Testing and Materials (ASTM) standard methods, before and after the pretreatment. The sample of crude glycerol comprised 75 wt % glycerol, 15% soap, 8% moisture, 0.8% methanol, and less than 5% ash. FFAs generated from crude glycerol were removed completely by microfiltration.
| Original language | English |
|---|---|
| Pages (from-to) | 12565-12573 |
| Number of pages | 9 |
| Journal | Industrial and Engineering Chemistry Research |
| Volume | 57 |
| Issue number | 37 |
| DOIs | |
| Publication status | Published - 19 Sep 2018 |
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ASJC Scopus subject areas
- Chemistry(all)
- Chemical Engineering(all)
- Industrial and Manufacturing Engineering
Cite this
Improved Fermentability of Pretreated Glycerol Enhanced Bioconversion of 1,3-Propanediol. / Tan, Jian Ping; Tee, Zhao Kang; Wan Nor Roslam, Wan Isahak; Kim, Byung Hong; Asis, Ahmad Jaril; Md Jahim, Jamaliah.
In: Industrial and Engineering Chemistry Research, Vol. 57, No. 37, 19.09.2018, p. 12565-12573.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improved Fermentability of Pretreated Glycerol Enhanced Bioconversion of 1,3-Propanediol
AU - Tan, Jian Ping
AU - Tee, Zhao Kang
AU - Wan Nor Roslam, Wan Isahak
AU - Kim, Byung Hong
AU - Asis, Ahmad Jaril
AU - Md Jahim, Jamaliah
PY - 2018/9/19
Y1 - 2018/9/19
N2 - The potential of biodiesel-derived glycerol to be valorized into 1,3-propanediol (1,3-PDO) was hindered by the impurities contained therein. This study introduced a straightforward and effective pretreatment process through microfiltration to remove free fatty acids (FFAs) that could inhibit the fermentation which resulted in remarkable high productivity in batch fermentation. This approach appreciably reduces the cost of pretreatment as compared to previously reported acidification, solvent extraction, electrodialysis, activated carbon adsorption and ion-exchange resins. From the fermentation results, a high productivity of 1,3-PDO of 2.16 g L-1 h-1 was attained when 20 g/L pretreated glycerol was used as the carbon source, an improvement of 62.4% compared with fermentation with crude glycerol using locally isolated strain Clostridium butyricum JKT37, and among the highest compared to previous reports. These results suggested that, with a simple microfiltration pretreatment, the utility of the resultant biodiesel-derived glycerol in bioproduction of 1,3-PDO is comparable to that of pure glycerol. The physical and chemical properties of the biodiesel-derived glycerol were characterized in accordance with American Oil Chemists' Society (AOCS), International Organization for Standardization (ISO), and American Society for Testing and Materials (ASTM) standard methods, before and after the pretreatment. The sample of crude glycerol comprised 75 wt % glycerol, 15% soap, 8% moisture, 0.8% methanol, and less than 5% ash. FFAs generated from crude glycerol were removed completely by microfiltration.
AB - The potential of biodiesel-derived glycerol to be valorized into 1,3-propanediol (1,3-PDO) was hindered by the impurities contained therein. This study introduced a straightforward and effective pretreatment process through microfiltration to remove free fatty acids (FFAs) that could inhibit the fermentation which resulted in remarkable high productivity in batch fermentation. This approach appreciably reduces the cost of pretreatment as compared to previously reported acidification, solvent extraction, electrodialysis, activated carbon adsorption and ion-exchange resins. From the fermentation results, a high productivity of 1,3-PDO of 2.16 g L-1 h-1 was attained when 20 g/L pretreated glycerol was used as the carbon source, an improvement of 62.4% compared with fermentation with crude glycerol using locally isolated strain Clostridium butyricum JKT37, and among the highest compared to previous reports. These results suggested that, with a simple microfiltration pretreatment, the utility of the resultant biodiesel-derived glycerol in bioproduction of 1,3-PDO is comparable to that of pure glycerol. The physical and chemical properties of the biodiesel-derived glycerol were characterized in accordance with American Oil Chemists' Society (AOCS), International Organization for Standardization (ISO), and American Society for Testing and Materials (ASTM) standard methods, before and after the pretreatment. The sample of crude glycerol comprised 75 wt % glycerol, 15% soap, 8% moisture, 0.8% methanol, and less than 5% ash. FFAs generated from crude glycerol were removed completely by microfiltration.
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U2 - 10.1021/acs.iecr.8b02268
DO - 10.1021/acs.iecr.8b02268
M3 - Article
AN - SCOPUS:85053300645
VL - 57
SP - 12565
EP - 12573
JO - Industrial & Engineering Chemistry Research
JF - Industrial & Engineering Chemistry Research
SN - 0888-5885
IS - 37
ER -