Impedimetric graphene-based biosensor for the detection of Escherichia coli DNA

Nurulasma Zainudin, Ab Rahim Mohd Hairul, Mashitah Mohd Yusoff, Ling Ling Tan @ Chong, Kwok Feng Chong

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A label-free impedimetric DNA biosensor based on graphene nanosheets has been developed for the detection of Escherichia coli O157:H7 strain GZ-021210. Probe DNA (pDNA) of E. coli was immobilized onto graphene nanosheets by the surface functionalization of graphene with 1-pyrenebutyric acid (PyBA) followed by carbodiimide linkage. The hybridization of complementary DNA (cDNA) of E. coli with the immobilized pDNA increased the electron transfer resistance of the graphene nanosheets, as observed by electrochemical impedance spectroscopy (EIS). The E. coli DNA biosensor displayed a wide range of linear response (1.0 × 10-10M to 1.0 × 10-14M), low detection limit (0.7 × 10-15M), single-base mismatch selectivity, high robustness and good reproducibility. The current work demonstrates an important advancement in the development of a sensitive biosensor for E. coli detection. This journal is

Original languageEnglish
Pages (from-to)7935-7941
Number of pages7
JournalAnalytical Methods
Volume6
Issue number19
DOIs
Publication statusPublished - 7 Oct 2014

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Biosensors
Graphene
Escherichia coli
DNA
Nanosheets
DNA Probes
Carbodiimides
Electrochemical impedance spectroscopy
Labels
Complementary DNA
Acids
Electrons

ASJC Scopus subject areas

  • Analytical Chemistry
  • Engineering(all)
  • Chemical Engineering(all)

Cite this

Impedimetric graphene-based biosensor for the detection of Escherichia coli DNA. / Zainudin, Nurulasma; Mohd Hairul, Ab Rahim; Yusoff, Mashitah Mohd; Tan @ Chong, Ling Ling; Chong, Kwok Feng.

In: Analytical Methods, Vol. 6, No. 19, 07.10.2014, p. 7935-7941.

Research output: Contribution to journalArticle

Zainudin, Nurulasma ; Mohd Hairul, Ab Rahim ; Yusoff, Mashitah Mohd ; Tan @ Chong, Ling Ling ; Chong, Kwok Feng. / Impedimetric graphene-based biosensor for the detection of Escherichia coli DNA. In: Analytical Methods. 2014 ; Vol. 6, No. 19. pp. 7935-7941.
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