Impact of pH and butyric acid on butanol production during batch fermentation using a new local isolate of Clostridium acetobutylicum YM1

Najeeb Kaid Nasser Al-Shorgani, Mohd. Sahaid Kalil, Wan Mohtar Wan Yusoff, Aidil Abdul Hamid

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The effect of pH and butyric acid supplementation on the production of butanol by a new local isolate of Clostridium acetobutylicum YM1 during batch culture fermentation was investigated. The results showed that pH had a significant effect on bacterial growth and butanol yield and productivity. The optimal initial pH that maximized butanol production was pH 6.0±0.2. Controlled pH was found to be unsuitable for butanol production in strain YM1, while the uncontrolled pH condition with an initial pH of 6.0±0.2 was suitable for bacterial growth, butanol yield and productivity. The maximum butanol concentration of 13.5±1.42g/L was obtained from cultures grown under the uncontrolled pH condition, resulting in a butanol yield (YP / S ) and productivity of 0.27g/g and 0.188g/Lh, respectively. Supplementation of the pH-controlled cultures with 4.0g/L butyric acid did not improve butanol production; however, supplementation of the uncontrolled pH cultures resulted in high butanol concentrations, yield and productivity (16.50±0.8g/L, 0.345g/g and 0.163g/Lh, respectively). pH influenced the activity of NADH-dependent butanol dehydrogenase, with the highest activity obtained under the uncontrolled pH condition. This study revealed that pH is a very important factor in butanol fermentation by C. acetobutylicum YM1.

Original languageEnglish
JournalSaudi Journal of Biological Sciences
DOIs
Publication statusAccepted/In press - 13 Jul 2015

Fingerprint

Clostridium acetobutylicum
batch fermentation
Butanols
Butyric Acid
butyric acid
butanol
Fermentation
microbial growth
fermentation
Batch Cell Culture Techniques
Growth

Keywords

  • Clostridium acetobutylicum YM1
  • Butanol
  • Butyric acid
  • NADH-dependent butanol dehydrogenase
  • PH

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

@article{79fa6bdcba664d9dbc8ea000d19ae2e6,
title = "Impact of pH and butyric acid on butanol production during batch fermentation using a new local isolate of Clostridium acetobutylicum YM1",
abstract = "The effect of pH and butyric acid supplementation on the production of butanol by a new local isolate of Clostridium acetobutylicum YM1 during batch culture fermentation was investigated. The results showed that pH had a significant effect on bacterial growth and butanol yield and productivity. The optimal initial pH that maximized butanol production was pH 6.0±0.2. Controlled pH was found to be unsuitable for butanol production in strain YM1, while the uncontrolled pH condition with an initial pH of 6.0±0.2 was suitable for bacterial growth, butanol yield and productivity. The maximum butanol concentration of 13.5±1.42g/L was obtained from cultures grown under the uncontrolled pH condition, resulting in a butanol yield (YP / S ) and productivity of 0.27g/g and 0.188g/Lh, respectively. Supplementation of the pH-controlled cultures with 4.0g/L butyric acid did not improve butanol production; however, supplementation of the uncontrolled pH cultures resulted in high butanol concentrations, yield and productivity (16.50±0.8g/L, 0.345g/g and 0.163g/Lh, respectively). pH influenced the activity of NADH-dependent butanol dehydrogenase, with the highest activity obtained under the uncontrolled pH condition. This study revealed that pH is a very important factor in butanol fermentation by C. acetobutylicum YM1.",
keywords = "Clostridium acetobutylicum YM1, Butanol, Butyric acid, NADH-dependent butanol dehydrogenase, PH",
author = "Al-Shorgani, {Najeeb Kaid Nasser} and Kalil, {Mohd. Sahaid} and {Wan Yusoff}, {Wan Mohtar} and {Abdul Hamid}, Aidil",
year = "2015",
month = "7",
day = "13",
doi = "10.1016/j.sjbs.2017.03.020",
language = "English",
journal = "Saudi Journal of Biological Sciences",
issn = "1319-562X",
publisher = "King Saud University",

}

TY - JOUR

T1 - Impact of pH and butyric acid on butanol production during batch fermentation using a new local isolate of Clostridium acetobutylicum YM1

AU - Al-Shorgani, Najeeb Kaid Nasser

AU - Kalil, Mohd. Sahaid

AU - Wan Yusoff, Wan Mohtar

AU - Abdul Hamid, Aidil

PY - 2015/7/13

Y1 - 2015/7/13

N2 - The effect of pH and butyric acid supplementation on the production of butanol by a new local isolate of Clostridium acetobutylicum YM1 during batch culture fermentation was investigated. The results showed that pH had a significant effect on bacterial growth and butanol yield and productivity. The optimal initial pH that maximized butanol production was pH 6.0±0.2. Controlled pH was found to be unsuitable for butanol production in strain YM1, while the uncontrolled pH condition with an initial pH of 6.0±0.2 was suitable for bacterial growth, butanol yield and productivity. The maximum butanol concentration of 13.5±1.42g/L was obtained from cultures grown under the uncontrolled pH condition, resulting in a butanol yield (YP / S ) and productivity of 0.27g/g and 0.188g/Lh, respectively. Supplementation of the pH-controlled cultures with 4.0g/L butyric acid did not improve butanol production; however, supplementation of the uncontrolled pH cultures resulted in high butanol concentrations, yield and productivity (16.50±0.8g/L, 0.345g/g and 0.163g/Lh, respectively). pH influenced the activity of NADH-dependent butanol dehydrogenase, with the highest activity obtained under the uncontrolled pH condition. This study revealed that pH is a very important factor in butanol fermentation by C. acetobutylicum YM1.

AB - The effect of pH and butyric acid supplementation on the production of butanol by a new local isolate of Clostridium acetobutylicum YM1 during batch culture fermentation was investigated. The results showed that pH had a significant effect on bacterial growth and butanol yield and productivity. The optimal initial pH that maximized butanol production was pH 6.0±0.2. Controlled pH was found to be unsuitable for butanol production in strain YM1, while the uncontrolled pH condition with an initial pH of 6.0±0.2 was suitable for bacterial growth, butanol yield and productivity. The maximum butanol concentration of 13.5±1.42g/L was obtained from cultures grown under the uncontrolled pH condition, resulting in a butanol yield (YP / S ) and productivity of 0.27g/g and 0.188g/Lh, respectively. Supplementation of the pH-controlled cultures with 4.0g/L butyric acid did not improve butanol production; however, supplementation of the uncontrolled pH cultures resulted in high butanol concentrations, yield and productivity (16.50±0.8g/L, 0.345g/g and 0.163g/Lh, respectively). pH influenced the activity of NADH-dependent butanol dehydrogenase, with the highest activity obtained under the uncontrolled pH condition. This study revealed that pH is a very important factor in butanol fermentation by C. acetobutylicum YM1.

KW - Clostridium acetobutylicum YM1

KW - Butanol

KW - Butyric acid

KW - NADH-dependent butanol dehydrogenase

KW - PH

UR - http://www.scopus.com/inward/record.url?scp=85019139745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019139745&partnerID=8YFLogxK

U2 - 10.1016/j.sjbs.2017.03.020

DO - 10.1016/j.sjbs.2017.03.020

M3 - Article

AN - SCOPUS:85019139745

JO - Saudi Journal of Biological Sciences

JF - Saudi Journal of Biological Sciences

SN - 1319-562X

ER -