Impact of low oxygen tension on stemness, proliferation and differentiation potential of human adipose-derived stem cells

Jane Ru Choi, Belinda Pingguan-Murphy, Wan Abu Bakar Wan Abas, Mat Adenan Noor Azmi, Siti Zawiah Omar, Chua Kien Hui, Wan Kamarul Zaman Wan Safwani

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Adipose-derived stem cells (ASCs) have been found adapted to a specific niche with low oxygen tension (hypoxia) in the body. As an important component of this niche, oxygen tension has been known to play a critical role in the maintenance of stem cell characteristics. However, the effect of O2 tension on their functional properties has not been well determined. In this study, we investigated the effects of O2 tension on ASCs stemness, differentiation and proliferation ability. Human ASCs were cultured under normoxia (21% O2) and hypoxia (2% O2). We found that hypoxia increased ASC stemness marker expression and proliferation rate without altering their morphology and surface markers. Low oxygen tension further enhances the chondrogenic differentiation ability, but reduces both adipogenic and osteogenic differentiation potential. These results might be correlated with the increased expression of HIF-1α under hypoxia. Taken together, we suggest that growing ASCs under 2% O2 tension may be important in expanding ASCs effectively while maintaining their functional properties for clinical therapy, particularly for the treatment of cartilage defects.

Original languageEnglish
Pages (from-to)218-224
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume448
Issue number2
DOIs
Publication statusPublished - 30 May 2014

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Stem cells
Stem Cells
Oxygen
Cartilage
Cell Differentiation
Maintenance
Cell Proliferation
Defects
Hypoxia

Keywords

  • Adipose-derived stem cells
  • Differentiation
  • Hypoxia
  • Proliferation
  • Stemness

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Impact of low oxygen tension on stemness, proliferation and differentiation potential of human adipose-derived stem cells. / Choi, Jane Ru; Pingguan-Murphy, Belinda; Wan Abas, Wan Abu Bakar; Noor Azmi, Mat Adenan; Omar, Siti Zawiah; Kien Hui, Chua; Wan Safwani, Wan Kamarul Zaman.

In: Biochemical and Biophysical Research Communications, Vol. 448, No. 2, 30.05.2014, p. 218-224.

Research output: Contribution to journalArticle

Choi, Jane Ru ; Pingguan-Murphy, Belinda ; Wan Abas, Wan Abu Bakar ; Noor Azmi, Mat Adenan ; Omar, Siti Zawiah ; Kien Hui, Chua ; Wan Safwani, Wan Kamarul Zaman. / Impact of low oxygen tension on stemness, proliferation and differentiation potential of human adipose-derived stem cells. In: Biochemical and Biophysical Research Communications. 2014 ; Vol. 448, No. 2. pp. 218-224.
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