Impact of exogenous ascorbic acid on biochemical activities of rice callus treated with salt stress

Arshad Naji Alhasnawi, Che Radziah Che Mohd. Zain, Ahsan A. Kadhimi, Anizan Isahak, Azhar Mohamad, Mehdi Farshad Ashraf, Febri Doni, Wan Mohtar Wan Yusoff

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The application of in vitro systems can lead to new methods of crop amelioration. This method has been widely utilized for breeding tenacities, particularly for stress tolerance selection. Salinity causes oxidative stress in callus by enhancing the production of Reactive Oxygen Species (ROS), resulting in an efficient antioxidant system. The exogenous application of ascorbic acid (AsA) is an important requirement for tolerance. The present study aimed to examine in vitro selection strategy for callus induction in rice mature embryo culture on MS culture medium and to produce salt-tolerant callus under sodium chloride (NaCl) and AsA conditions in callus rice variety, MR269. This study also highlights changes in the activities of proline and antioxidants peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) of callus under NaCl stress to understand their possible role in salt tolerance. However, various levels of exogenously applied AsA under saline conditions improved callus, and the antioxidant enzyme activities of AsA are related to resistance to oxidative stress. Our results provide strong support for the hypothesis that AsA-dependent antioxidant enzymes play a significant role in the salinity tolerance of callus rice.

Original languageEnglish
Title of host publication2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1784
ISBN (Electronic)9780735414464
DOIs
Publication statusPublished - 17 Nov 2016
Event2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016 - Selangor, Malaysia
Duration: 13 Apr 201614 Apr 2016

Other

Other2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016
CountryMalaysia
CitySelangor
Period13/4/1614/4/16

Fingerprint

ascorbic acid
rice
antioxidants
salts
salinity
catalase
culture media
enzyme activity
inorganic peroxides
embryos
crops
sodium chlorides
enzymes
induction
requirements
causes
oxygen

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Alhasnawi, A. N., Che Mohd. Zain, C. R., Kadhimi, A. A., Isahak, A., Mohamad, A., Ashraf, M. F., ... Wan Yusoff, W. M. (2016). Impact of exogenous ascorbic acid on biochemical activities of rice callus treated with salt stress. In 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium (Vol. 1784). [020005] American Institute of Physics Inc.. https://doi.org/10.1063/1.4966715

Impact of exogenous ascorbic acid on biochemical activities of rice callus treated with salt stress. / Alhasnawi, Arshad Naji; Che Mohd. Zain, Che Radziah; Kadhimi, Ahsan A.; Isahak, Anizan; Mohamad, Azhar; Ashraf, Mehdi Farshad; Doni, Febri; Wan Yusoff, Wan Mohtar.

2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784 American Institute of Physics Inc., 2016. 020005.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Alhasnawi, AN, Che Mohd. Zain, CR, Kadhimi, AA, Isahak, A, Mohamad, A, Ashraf, MF, Doni, F & Wan Yusoff, WM 2016, Impact of exogenous ascorbic acid on biochemical activities of rice callus treated with salt stress. in 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. vol. 1784, 020005, American Institute of Physics Inc., 2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016, Selangor, Malaysia, 13/4/16. https://doi.org/10.1063/1.4966715
Alhasnawi AN, Che Mohd. Zain CR, Kadhimi AA, Isahak A, Mohamad A, Ashraf MF et al. Impact of exogenous ascorbic acid on biochemical activities of rice callus treated with salt stress. In 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784. American Institute of Physics Inc. 2016. 020005 https://doi.org/10.1063/1.4966715
Alhasnawi, Arshad Naji ; Che Mohd. Zain, Che Radziah ; Kadhimi, Ahsan A. ; Isahak, Anizan ; Mohamad, Azhar ; Ashraf, Mehdi Farshad ; Doni, Febri ; Wan Yusoff, Wan Mohtar. / Impact of exogenous ascorbic acid on biochemical activities of rice callus treated with salt stress. 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784 American Institute of Physics Inc., 2016.
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