Impact of adipogenic differentiation on stemness and osteogenic gene expression in extensive culture of human adipose-derived stem cells

Wan Kamarul Zaman Wan Safwani, Suzana Makpol, Somasundaram Sathapan, Chua Kien Hui

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Introduction: Adipose tissue is a source of multipotent adult stem cells. Most studies on human adipose-derived stem cells (ASC) have been on the early passages. Studies in extensive expansion have not been well established yet. In this study, we aim to investigate the effects of extensive expansion on the adipogenic differentiation capability of ASC. Material and methods: The ability of ASC to undergo adipogenic differentiation in extensive expansion was evaluated by morphological changes, differentiation assay by using Oil Red O staining and changes in the genes expression levels of adipogenic genes, osteogenic genes and stemness genes using quantitative polymerase chain reaction (qPCR) after induction. Results: Morphological study showed that the formation of lipid droplets can be observed at all passages but decreased at P20 after induction. Data from qPCR showed that most adipogenicgenes expression increased significantlyat P5, P10 and P15 but decreased at P20 after induction. On the other hand, osteogenic genes showed no significant changes after adipogenic induction indicating low potentiality of adipogenic-induced ASC to become osteogenic cells. While stemness genes expression levels showed a decrease or no significant changes after adipogenic induction except Nanog3, which showed a significant increase at P15 and P20. Conclusions: The ability of ASC to differentiate into mature adipogenic cells decreased after P10 and the decrease in the osteogenics gene expression level during adipogenic induction suggested that the osteogenesis and adipogenesis are not parallel events.

Original languageEnglish
Pages (from-to)597-606
Number of pages10
JournalArchives of Medical Science
Volume10
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Stem Cells
Gene Expression
Genes
Multipotent Stem Cells
Adipogenesis
Polymerase Chain Reaction
Adult Stem Cells
Osteogenesis
Adipose Tissue
Staining and Labeling

Keywords

  • Adipogenesis
  • Adipose
  • Cell-based therapy
  • Culture expansion
  • Differentiation
  • Osteogenesis
  • Stem cells
  • Stemness

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Impact of adipogenic differentiation on stemness and osteogenic gene expression in extensive culture of human adipose-derived stem cells. / Safwani, Wan Kamarul Zaman Wan; Makpol, Suzana; Sathapan, Somasundaram; Kien Hui, Chua.

In: Archives of Medical Science, Vol. 10, No. 3, 2014, p. 597-606.

Research output: Contribution to journalArticle

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