Immunosuppressive phenolic compounds from Hydnora abyssinica A. Braun

Waleed S. Koko, Osman Mesaik Mohammed Ahmed Hassan, Rosa Ranjitt, Mohamed Galal, Muhammad I. Choudhary

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Hydnora abyssinica (HA) A. Braun is an endemic Sudanese medicinal plant traditionally used as anti-inflammatory and against many infectious diseases. However, it proved to be very rich in phenols and tannins, so the present study was undertaken to investigate the immunomodulatory potential of the whole plant ethanolic extract and its isolated compounds. Methods: Lymphocyte proliferation, chemiluminescence and superoxide reduction assays were used for immunomodulatory evaluation. While, MTT (3-(4, 5-dimethylthazol-2-yl)-2, 5-diphenyl tetrazonium bromide) test was performed on 3T3 cell line clone in order to evaluate the cytoxicity effect of the extracts and isolated compounds of phenolic derivatives which were carried out by chromotographic techniques. Results: Catechin, (1), tyrosol (2) and benzoic acid, 3, 4, dihydroxy-, ethyl ester (3) compounds were isolated from HA ethanolic extract which revealed potent immunosuppressive activity against reactive oxygen species from both polymorph nuclear cells (PMNs) (45-90% inhibition) and mononuclear cells (MNCs) (30 -65% inhibition), T lymphocyte proliferation assay (70-93 % inhibition) as well as potent inhibitory effect against superoxide production (42-71% inhibition) at concentrations of 6.25-100μg/mL. Catechin (1) was found the most potent immunosuppressive agent among all constituents examined. Conclusion: These results can support the traditional uses of H. abyssinica extracts as anti-inflammatory and immunosuppressive and further investigations of the mode of action and other pharmacological studies are highly desirable.

Original languageEnglish
Article number400
JournalBMC Complementary and Alternative Medicine
Volume15
Issue number1
DOIs
Publication statusPublished - 9 Nov 2015

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Immunosuppressive Agents
Catechin
Superoxides
Anti-Inflammatory Agents
3T3 Cells
Benzoic Acid
Tannins
Phenols
Plant Extracts
Medicinal Plants
Luminescence
Bromides
Communicable Diseases
Reactive Oxygen Species
Esters
Clone Cells
Pharmacology
Lymphocytes
T-Lymphocytes
Cell Line

Keywords

  • Chemiluminescence assay
  • Hydnora abyssinica
  • Immunosuppression
  • Oxidative burst

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Immunosuppressive phenolic compounds from Hydnora abyssinica A. Braun. / Koko, Waleed S.; Mohammed Ahmed Hassan, Osman Mesaik; Ranjitt, Rosa; Galal, Mohamed; Choudhary, Muhammad I.

In: BMC Complementary and Alternative Medicine, Vol. 15, No. 1, 400, 09.11.2015.

Research output: Contribution to journalArticle

Koko, Waleed S. ; Mohammed Ahmed Hassan, Osman Mesaik ; Ranjitt, Rosa ; Galal, Mohamed ; Choudhary, Muhammad I. / Immunosuppressive phenolic compounds from Hydnora abyssinica A. Braun. In: BMC Complementary and Alternative Medicine. 2015 ; Vol. 15, No. 1.
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