Immunosuppressive effects of the standardized extract of Zingiber zerumbet on innate immune responses in Wistar rats

Nor Shazliana Ghazalee, Ibrahim Jantan, Laiba Arshad, Md Areeful Haque

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Zingiber zerumbet rhizome has been used in traditional medicine mainly for the treatment of various immune-inflammatory related ailments and has been shown to exhibit a wide spectrum of biological effects especially antioxidant and anti-inflammatory activities. The present study was aimed to investigate the immunosuppressive effects of the standardized 80% ethanol extract of Z. zerumbet at 100, 200, and 400 mg/kg on the innate immune responses in male Wistar rats. The immune parameters determined were chemotaxis of neutrophils, Mac-1 expression, engulfment of Escherichia coli by neutrophils, reactive oxygen species production, and plasma lysozyme and ceruloplasmin levels. Zerumbone was qualitatively and quantitatively determined in the extract by using a validated reversed-phase HPLC, whereas liquid chromatography tandem-mass spectrometry (LC -MS/MS) was used to profile the secondary metabolites. Z. zerumbet significantly inhibited the migration of neutrophils, expressions of CD11b/CD18 integrin, phagocytic activity, and production of reactive oxygen species in a dose-dependent manner. The extract also dose-dependently inhibited the expressions of lysozyme and ceruloplasmin in the rat plasma. Z. zerumbet extract possessed strong inhibitory effects on the innate immune responses and has potential to be developed into an effective immunosuppressive agent.

Original languageEnglish
JournalPhytotherapy Research
DOIs
Publication statusAccepted/In press - 1 Jan 2019
Externally publishedYes

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Immunosuppressive Agents
Innate Immunity
Wistar Rats
Ceruloplasmin
Neutrophils
Muramidase
Reactive Oxygen Species
Rhizome
Traditional Medicine
Chemotaxis
Tandem Mass Spectrometry
Integrins
Liquid Chromatography
Anti-Inflammatory Agents
Ethanol
Antioxidants
High Pressure Liquid Chromatography
Escherichia coli
Therapeutics

Keywords

  • immunosuppression
  • innate immunity
  • neutrophil migration
  • phagocytosis
  • reactive oxygen species
  • Zingiber zerumbet

ASJC Scopus subject areas

  • Pharmacology

Cite this

Immunosuppressive effects of the standardized extract of Zingiber zerumbet on innate immune responses in Wistar rats. / Ghazalee, Nor Shazliana; Jantan, Ibrahim; Arshad, Laiba; Haque, Md Areeful.

In: Phytotherapy Research, 01.01.2019.

Research output: Contribution to journalArticle

Ghazalee, Nor Shazliana ; Jantan, Ibrahim ; Arshad, Laiba ; Haque, Md Areeful. / Immunosuppressive effects of the standardized extract of Zingiber zerumbet on innate immune responses in Wistar rats. In: Phytotherapy Research. 2019.
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