Immunosuppressive activity of buxidin and E-buxenone from buxus hyrcana

Osman Mesaik Mohammed Ahmed Hassan, Sobia A. Halim, Zaheer Ul-Haq, M. Iqbal Choudhary, Salma Shahnaz, S. A M Ayatollahi, Shahnaz Murad, Aqeel Ahmad

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Buxidin (1) and E-Buxenone (2), steroidal alkaloids from Buxus hyrcana, are found to possess potent immunosuppressive properties. The activity was tested in vitro on oxidative burst, chemotaxis, T-cell proliferation, and cytokine production. Both compounds showed a significant immunomodulatory activity with clear suppressive effect on oxidative burst and chemotaxis in a dose-dependent manner. They also exhibited suppressive effect on the phytohemagglutinin- stimulated T-cell proliferation. The immunomodulatory activity was further confirmed by the suppression of IL-2 and IL-4 production. Furthermore, molecular docking studies were performed to investigate the binding mode of Buxidin (1) and E-Buxenone (2) with IL-2. Despite the structural differences between Buxidin (1) and E-Buxenone (2), docking results revealed that they adopt a similar binding pattern at the active site of the IL-2. A good agreement between practical and theoretic results indicates that the current docking study could provide an alternate tool for the structural optimization of recently identified ligand as more potent IL-2 inhibitors.

Original languageEnglish
Pages (from-to)310-317
Number of pages8
JournalChemical Biology and Drug Design
Volume75
Issue number3
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Buxus
Immunosuppressive Agents
Interleukin-2
Respiratory Burst
T-cells
Cell proliferation
Chemotaxis
Cell Proliferation
T-Lymphocytes
Structural optimization
Phytohemagglutinins
Alkaloids
Interleukin-4
Catalytic Domain
Cytokines
Ligands
E-buxenone
buxidin

Keywords

  • Buxidin
  • E-Buxenone
  • FlexX
  • Immunosuppressive agents
  • Molecular docking

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Medicine(all)

Cite this

Mohammed Ahmed Hassan, O. M., Halim, S. A., Ul-Haq, Z., Choudhary, M. I., Shahnaz, S., Ayatollahi, S. A. M., ... Ahmad, A. (2010). Immunosuppressive activity of buxidin and E-buxenone from buxus hyrcana. Chemical Biology and Drug Design, 75(3), 310-317. https://doi.org/10.1111/j.1747-0285.2009.00906.x

Immunosuppressive activity of buxidin and E-buxenone from buxus hyrcana. / Mohammed Ahmed Hassan, Osman Mesaik; Halim, Sobia A.; Ul-Haq, Zaheer; Choudhary, M. Iqbal; Shahnaz, Salma; Ayatollahi, S. A M; Murad, Shahnaz; Ahmad, Aqeel.

In: Chemical Biology and Drug Design, Vol. 75, No. 3, 2010, p. 310-317.

Research output: Contribution to journalArticle

Mohammed Ahmed Hassan, OM, Halim, SA, Ul-Haq, Z, Choudhary, MI, Shahnaz, S, Ayatollahi, SAM, Murad, S & Ahmad, A 2010, 'Immunosuppressive activity of buxidin and E-buxenone from buxus hyrcana', Chemical Biology and Drug Design, vol. 75, no. 3, pp. 310-317. https://doi.org/10.1111/j.1747-0285.2009.00906.x
Mohammed Ahmed Hassan OM, Halim SA, Ul-Haq Z, Choudhary MI, Shahnaz S, Ayatollahi SAM et al. Immunosuppressive activity of buxidin and E-buxenone from buxus hyrcana. Chemical Biology and Drug Design. 2010;75(3):310-317. https://doi.org/10.1111/j.1747-0285.2009.00906.x
Mohammed Ahmed Hassan, Osman Mesaik ; Halim, Sobia A. ; Ul-Haq, Zaheer ; Choudhary, M. Iqbal ; Shahnaz, Salma ; Ayatollahi, S. A M ; Murad, Shahnaz ; Ahmad, Aqeel. / Immunosuppressive activity of buxidin and E-buxenone from buxus hyrcana. In: Chemical Biology and Drug Design. 2010 ; Vol. 75, No. 3. pp. 310-317.
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