Immunostimulant activity of standardised extracts of mangifera indica leaf and curcuma domestica rhizome in mice

Kumolosasi Msi Endang, Siti Nur Aisyah Ibrahim, Siti Munirah Ahmad Shukri, Waqas Ahmad

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: To investigate the immunomodulating activity of Mangifera indica. (MI) and Curcuma domestica (CD) extracts in mice. Methods: Mice were randomly divided into 4 groups, namely, vehicle, untreated, MI and CD groups (n = 6). They were treated with MI (160 mg/kg) or CD (200 mg/kg) extracts, and vehicle for control group, for 14 days and sacrificed on day 15. In innate immunity test, the mice were challenged with sheep red blood cells (SRBCs) antigen on day 8, while in adaptive immunity test, mice were immunized and challenged on days 7 and 14, respectively, with SRBC. White blood cells count (WBC), spleen index (SI), and haemagglutination (HA) titer, and delayed type hypersensitivity (DTH) responses were determined. Results: For both plant extracts, adaptive immunity groups showed the highest response compared to innate groups. In adaptive immunity, the WBC count of MI and CD treated animals was significantly higher than in the untreated and vehicle treated groups (p < 0.001). Moreover, the SI of mice from MI and CD treated groups differed significantly that of the untreated group (p < 0.01 and p < 0.05, respectively). HA titer in CD (both non-challenged and challenged) groups was significantly higher than in the non-challenged vehicle group (p < 0.001). HA titer in MI group (non-challenged) was significantly lower than in non-challenged CD and challenged groups (p < 0.01 and p < 0.001, respectively). Conclusion: MI and CD extracts, in appropriate doses, exerted immunostimulant effects in mice by enhancing both innate and adaptive immune systems via increase in WBC, SI, HA titer and DTH responses.

Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalTropical Journal of Pharmaceutical Research
Volume17
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Immunologic Adjuvants
Curcuma
Rhizome
Hemagglutination
Adaptive Immunity
Leukocyte Count
Spleen
Delayed Hypersensitivity
Sheep
Erythrocytes
Mangifera
Mangifera indica extract
Plant Extracts
Innate Immunity
Immune System
Antigens
Control Groups

Keywords

  • Curcuma domestica
  • Haemagglutination
  • Hypersensitivity response
  • Immunostimulant activity
  • Mangifera indica
  • Spleen index

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

Immunostimulant activity of standardised extracts of mangifera indica leaf and curcuma domestica rhizome in mice. / Endang, Kumolosasi Msi; Ibrahim, Siti Nur Aisyah; Shukri, Siti Munirah Ahmad; Ahmad, Waqas.

In: Tropical Journal of Pharmaceutical Research, Vol. 17, No. 1, 01.01.2018, p. 77-84.

Research output: Contribution to journalArticle

@article{0b4821dfe8464470ac79b5e62a22dc90,
title = "Immunostimulant activity of standardised extracts of mangifera indica leaf and curcuma domestica rhizome in mice",
abstract = "Purpose: To investigate the immunomodulating activity of Mangifera indica. (MI) and Curcuma domestica (CD) extracts in mice. Methods: Mice were randomly divided into 4 groups, namely, vehicle, untreated, MI and CD groups (n = 6). They were treated with MI (160 mg/kg) or CD (200 mg/kg) extracts, and vehicle for control group, for 14 days and sacrificed on day 15. In innate immunity test, the mice were challenged with sheep red blood cells (SRBCs) antigen on day 8, while in adaptive immunity test, mice were immunized and challenged on days 7 and 14, respectively, with SRBC. White blood cells count (WBC), spleen index (SI), and haemagglutination (HA) titer, and delayed type hypersensitivity (DTH) responses were determined. Results: For both plant extracts, adaptive immunity groups showed the highest response compared to innate groups. In adaptive immunity, the WBC count of MI and CD treated animals was significantly higher than in the untreated and vehicle treated groups (p < 0.001). Moreover, the SI of mice from MI and CD treated groups differed significantly that of the untreated group (p < 0.01 and p < 0.05, respectively). HA titer in CD (both non-challenged and challenged) groups was significantly higher than in the non-challenged vehicle group (p < 0.001). HA titer in MI group (non-challenged) was significantly lower than in non-challenged CD and challenged groups (p < 0.01 and p < 0.001, respectively). Conclusion: MI and CD extracts, in appropriate doses, exerted immunostimulant effects in mice by enhancing both innate and adaptive immune systems via increase in WBC, SI, HA titer and DTH responses.",
keywords = "Curcuma domestica, Haemagglutination, Hypersensitivity response, Immunostimulant activity, Mangifera indica, Spleen index",
author = "Endang, {Kumolosasi Msi} and Ibrahim, {Siti Nur Aisyah} and Shukri, {Siti Munirah Ahmad} and Waqas Ahmad",
year = "2018",
month = "1",
day = "1",
doi = "10.4314/tjpr.v17i1.12",
language = "English",
volume = "17",
pages = "77--84",
journal = "Tropical Journal of Pharmaceutical Research",
issn = "1596-5996",
publisher = "Pharmacotherapy Group",
number = "1",

}

TY - JOUR

T1 - Immunostimulant activity of standardised extracts of mangifera indica leaf and curcuma domestica rhizome in mice

AU - Endang, Kumolosasi Msi

AU - Ibrahim, Siti Nur Aisyah

AU - Shukri, Siti Munirah Ahmad

AU - Ahmad, Waqas

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Purpose: To investigate the immunomodulating activity of Mangifera indica. (MI) and Curcuma domestica (CD) extracts in mice. Methods: Mice were randomly divided into 4 groups, namely, vehicle, untreated, MI and CD groups (n = 6). They were treated with MI (160 mg/kg) or CD (200 mg/kg) extracts, and vehicle for control group, for 14 days and sacrificed on day 15. In innate immunity test, the mice were challenged with sheep red blood cells (SRBCs) antigen on day 8, while in adaptive immunity test, mice were immunized and challenged on days 7 and 14, respectively, with SRBC. White blood cells count (WBC), spleen index (SI), and haemagglutination (HA) titer, and delayed type hypersensitivity (DTH) responses were determined. Results: For both plant extracts, adaptive immunity groups showed the highest response compared to innate groups. In adaptive immunity, the WBC count of MI and CD treated animals was significantly higher than in the untreated and vehicle treated groups (p < 0.001). Moreover, the SI of mice from MI and CD treated groups differed significantly that of the untreated group (p < 0.01 and p < 0.05, respectively). HA titer in CD (both non-challenged and challenged) groups was significantly higher than in the non-challenged vehicle group (p < 0.001). HA titer in MI group (non-challenged) was significantly lower than in non-challenged CD and challenged groups (p < 0.01 and p < 0.001, respectively). Conclusion: MI and CD extracts, in appropriate doses, exerted immunostimulant effects in mice by enhancing both innate and adaptive immune systems via increase in WBC, SI, HA titer and DTH responses.

AB - Purpose: To investigate the immunomodulating activity of Mangifera indica. (MI) and Curcuma domestica (CD) extracts in mice. Methods: Mice were randomly divided into 4 groups, namely, vehicle, untreated, MI and CD groups (n = 6). They were treated with MI (160 mg/kg) or CD (200 mg/kg) extracts, and vehicle for control group, for 14 days and sacrificed on day 15. In innate immunity test, the mice were challenged with sheep red blood cells (SRBCs) antigen on day 8, while in adaptive immunity test, mice were immunized and challenged on days 7 and 14, respectively, with SRBC. White blood cells count (WBC), spleen index (SI), and haemagglutination (HA) titer, and delayed type hypersensitivity (DTH) responses were determined. Results: For both plant extracts, adaptive immunity groups showed the highest response compared to innate groups. In adaptive immunity, the WBC count of MI and CD treated animals was significantly higher than in the untreated and vehicle treated groups (p < 0.001). Moreover, the SI of mice from MI and CD treated groups differed significantly that of the untreated group (p < 0.01 and p < 0.05, respectively). HA titer in CD (both non-challenged and challenged) groups was significantly higher than in the non-challenged vehicle group (p < 0.001). HA titer in MI group (non-challenged) was significantly lower than in non-challenged CD and challenged groups (p < 0.01 and p < 0.001, respectively). Conclusion: MI and CD extracts, in appropriate doses, exerted immunostimulant effects in mice by enhancing both innate and adaptive immune systems via increase in WBC, SI, HA titer and DTH responses.

KW - Curcuma domestica

KW - Haemagglutination

KW - Hypersensitivity response

KW - Immunostimulant activity

KW - Mangifera indica

KW - Spleen index

UR - http://www.scopus.com/inward/record.url?scp=85041584446&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041584446&partnerID=8YFLogxK

U2 - 10.4314/tjpr.v17i1.12

DO - 10.4314/tjpr.v17i1.12

M3 - Article

VL - 17

SP - 77

EP - 84

JO - Tropical Journal of Pharmaceutical Research

JF - Tropical Journal of Pharmaceutical Research

SN - 1596-5996

IS - 1

ER -