Immunophenotyping of peroxidase-negative acute leukaemias using immunoalkaline phosphatase (APAAP) method.

S. K. Cheong, Y. C. Lim, O. Ainoon, Noor Hamidah Hussin

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Abstract

Immunophenotyping of acute leukaemias has become an important diagnostic tool in haematology laboratories as it is now well recognised that the presence of certain surface markers has prognostic significance. In 1988, we experimented with the alkaline phosphatase anti-alkaline phosphatase (APAAP) method for immunophenotyping of leukaemic cells in our laboratory. 48 cases of peroxidase-negative acute leukaemias were studied. Our study showed that 2 peroxidase-negative cases carried myeloid surface markers, 44% were negative for the markers studied and 5% were unclassified due to technical problems. We concluded that the APAAP method is a useful technique for demonstrating cell markers in leukaemic cells as the reaction is reddish and usually intense. We failed to demonstrate surface markers in 44% of the cases probably because of the choice of a limited panel of monoclonal antibodies.

Original languageEnglish
Pages (from-to)119-121
Number of pages3
JournalThe Malaysian journal of pathology
Volume13
Issue number2
Publication statusPublished - Dec 1991

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Immunophenotyping
Phosphoric Monoester Hydrolases
Peroxidase
Alkaline Phosphatase
Leukemia
Hematology
Monoclonal Antibodies

ASJC Scopus subject areas

  • Medicine(all)

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Immunophenotyping of peroxidase-negative acute leukaemias using immunoalkaline phosphatase (APAAP) method. / Cheong, S. K.; Lim, Y. C.; Ainoon, O.; Hussin, Noor Hamidah.

In: The Malaysian journal of pathology, Vol. 13, No. 2, 12.1991, p. 119-121.

Research output: Contribution to journalArticle

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