Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes

Nurul Hikmah Harun, Abdi Wira Septama, Ibrahim Jantan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: To investigate the effects of 20 methanolic extracts from Malaysian selected plants on CD18/11a expression and phagocytosis activity of leukocytes using flow cytometry analysis. Methods: The effects of methanolic extracts on CD18/11a expression and phagocytosis of leukocytes were measured by labelling the cells with CD18-fluorescein isothiocyanate and ingestion labelled with Escherichia coli-fluorescein isothiocyanate and then analyzed using flow cytometer. Results: About 12 out of 20 methanolic extracts of selected Malaysian medicinal plants significantly (P≤0.05) inhibited the CD18/11a expression of leukocytes at both concentrations of 6.25 μg/mL and 100 μg/mL in dose dependent manner. The most active inhibitory was shown in Citrus aurantifolia (Christm.) Swingle and Alpinia galangal (L.) Willd. at dosage 100 μg/mL. Moreover, the Orthosiphon aristatus (Blume) Miq (O. aristatus). showed the highest stimulatory activity at the concentration of 100 μg/mL. Other than that, four plant extracts significantly (P≤0.05) rose the phagocytosis activities of leukocytes in dose dependent manner. However, Annona muricata L. and O. aristatus showed the highest stimulated activities at the 100 μg/mL concentration. Conclusions: The results suggest that methanolic extracts of Citrus aurantifolia, Alpinia galangal, O. aristatus and Annona muricata are able to modulate innate immune system and can potentially be recognized as therapeutic agents for modulating immune system.

Original languageEnglish
Pages (from-to)48-53
Number of pages6
JournalAsian Pacific Journal of Tropical Biomedicine
Volume5
Issue number1
Publication statusPublished - 2015

Fingerprint

Orthosiphon
Immune system
Fluorescein
Phagocytosis
Leukocytes
Alpinia
Annona
Flow cytometry
Citrus
Plant Extracts
Labeling
Escherichia coli
Immune System
Medicinal Plants
Flow Cytometry
Eating
isothiocyanic acid

Keywords

  • CD18/11a expression
  • Flow cytometry analysis
  • Immunomodulatory effects
  • Phagocytosis activity
  • Selected Malaysian medicinal plants

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes. / Harun, Nurul Hikmah; Septama, Abdi Wira; Jantan, Ibrahim.

In: Asian Pacific Journal of Tropical Biomedicine, Vol. 5, No. 1, 2015, p. 48-53.

Research output: Contribution to journalArticle

@article{5e18a153e9394692a34034d3f00cd6d2,
title = "Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes",
abstract = "Objective: To investigate the effects of 20 methanolic extracts from Malaysian selected plants on CD18/11a expression and phagocytosis activity of leukocytes using flow cytometry analysis. Methods: The effects of methanolic extracts on CD18/11a expression and phagocytosis of leukocytes were measured by labelling the cells with CD18-fluorescein isothiocyanate and ingestion labelled with Escherichia coli-fluorescein isothiocyanate and then analyzed using flow cytometer. Results: About 12 out of 20 methanolic extracts of selected Malaysian medicinal plants significantly (P≤0.05) inhibited the CD18/11a expression of leukocytes at both concentrations of 6.25 μg/mL and 100 μg/mL in dose dependent manner. The most active inhibitory was shown in Citrus aurantifolia (Christm.) Swingle and Alpinia galangal (L.) Willd. at dosage 100 μg/mL. Moreover, the Orthosiphon aristatus (Blume) Miq (O. aristatus). showed the highest stimulatory activity at the concentration of 100 μg/mL. Other than that, four plant extracts significantly (P≤0.05) rose the phagocytosis activities of leukocytes in dose dependent manner. However, Annona muricata L. and O. aristatus showed the highest stimulated activities at the 100 μg/mL concentration. Conclusions: The results suggest that methanolic extracts of Citrus aurantifolia, Alpinia galangal, O. aristatus and Annona muricata are able to modulate innate immune system and can potentially be recognized as therapeutic agents for modulating immune system.",
keywords = "CD18/11a expression, Flow cytometry analysis, Immunomodulatory effects, Phagocytosis activity, Selected Malaysian medicinal plants",
author = "Harun, {Nurul Hikmah} and Septama, {Abdi Wira} and Ibrahim Jantan",
year = "2015",
language = "English",
volume = "5",
pages = "48--53",
journal = "Asian Pacific Journal of Tropical Biomedicine",
issn = "2221-1691",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes

AU - Harun, Nurul Hikmah

AU - Septama, Abdi Wira

AU - Jantan, Ibrahim

PY - 2015

Y1 - 2015

N2 - Objective: To investigate the effects of 20 methanolic extracts from Malaysian selected plants on CD18/11a expression and phagocytosis activity of leukocytes using flow cytometry analysis. Methods: The effects of methanolic extracts on CD18/11a expression and phagocytosis of leukocytes were measured by labelling the cells with CD18-fluorescein isothiocyanate and ingestion labelled with Escherichia coli-fluorescein isothiocyanate and then analyzed using flow cytometer. Results: About 12 out of 20 methanolic extracts of selected Malaysian medicinal plants significantly (P≤0.05) inhibited the CD18/11a expression of leukocytes at both concentrations of 6.25 μg/mL and 100 μg/mL in dose dependent manner. The most active inhibitory was shown in Citrus aurantifolia (Christm.) Swingle and Alpinia galangal (L.) Willd. at dosage 100 μg/mL. Moreover, the Orthosiphon aristatus (Blume) Miq (O. aristatus). showed the highest stimulatory activity at the concentration of 100 μg/mL. Other than that, four plant extracts significantly (P≤0.05) rose the phagocytosis activities of leukocytes in dose dependent manner. However, Annona muricata L. and O. aristatus showed the highest stimulated activities at the 100 μg/mL concentration. Conclusions: The results suggest that methanolic extracts of Citrus aurantifolia, Alpinia galangal, O. aristatus and Annona muricata are able to modulate innate immune system and can potentially be recognized as therapeutic agents for modulating immune system.

AB - Objective: To investigate the effects of 20 methanolic extracts from Malaysian selected plants on CD18/11a expression and phagocytosis activity of leukocytes using flow cytometry analysis. Methods: The effects of methanolic extracts on CD18/11a expression and phagocytosis of leukocytes were measured by labelling the cells with CD18-fluorescein isothiocyanate and ingestion labelled with Escherichia coli-fluorescein isothiocyanate and then analyzed using flow cytometer. Results: About 12 out of 20 methanolic extracts of selected Malaysian medicinal plants significantly (P≤0.05) inhibited the CD18/11a expression of leukocytes at both concentrations of 6.25 μg/mL and 100 μg/mL in dose dependent manner. The most active inhibitory was shown in Citrus aurantifolia (Christm.) Swingle and Alpinia galangal (L.) Willd. at dosage 100 μg/mL. Moreover, the Orthosiphon aristatus (Blume) Miq (O. aristatus). showed the highest stimulatory activity at the concentration of 100 μg/mL. Other than that, four plant extracts significantly (P≤0.05) rose the phagocytosis activities of leukocytes in dose dependent manner. However, Annona muricata L. and O. aristatus showed the highest stimulated activities at the 100 μg/mL concentration. Conclusions: The results suggest that methanolic extracts of Citrus aurantifolia, Alpinia galangal, O. aristatus and Annona muricata are able to modulate innate immune system and can potentially be recognized as therapeutic agents for modulating immune system.

KW - CD18/11a expression

KW - Flow cytometry analysis

KW - Immunomodulatory effects

KW - Phagocytosis activity

KW - Selected Malaysian medicinal plants

UR - http://www.scopus.com/inward/record.url?scp=84922739404&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922739404&partnerID=8YFLogxK

M3 - Article

VL - 5

SP - 48

EP - 53

JO - Asian Pacific Journal of Tropical Biomedicine

JF - Asian Pacific Journal of Tropical Biomedicine

SN - 2221-1691

IS - 1

ER -