Immunomodulatory activities of some common lichen metabolites

Vinitha M. Thadhani, Osman Mesaik Mohammed Ahmed Hassan, Muhammad Asif, Veranja Karunaratne, Iqbal M. Choudhary

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: To evaluate the immunomodulatory activities of some of the common lichen compounds by using chemiluminescence based cellular assays. Methods: Number of secondary lichen metabolites, representing a breadth of lichen substances, was investigated for their effects on the respiratory burst of human whole blood phagocytes, isolated human polymorphonuclear leukocytes (PMNs) and murine macrophages using luminol or lucigenin-based chemiluminescence probes. Results: This study identify a clear suppressive effect of some lichen metabolites on phagocytosis response upon activation with serum opsonized zymosan by several lichen substances. Amongst the compounds tested, orsellinic acid, methyl orsellinate, methyl haematomate, lecanoric acid and lobaric acid, showed a potent immunomodulatory activity as compared to the standards. The lobaric acid suppressed both the myloperoxidase dependent and myloperoxidase independent, Reactive Oxygen Species (ROS) production in the oxidative burst of polymorphonuclear neutrophils (PMN) at the lowest concentration tested (3.1 μg/ml). Whereas, lecanoric acid, suppressed only the myloperoxidase dependent ROS production with IC50<3.1μg/ml when compared to the standard sodium diethyldithiocarbamate trihydrate (SDT) (IC50 = 1.3 ± 0.2 μg/ml). Orsellinic acid, methyl orsellinate and methyl haematomate showed a selective myloperoxidase independent pathway with IC50 values; <3.1μg/ml; 6.1 ± 1.0 μg/ml; 3.3 ± 0.1 μg/ml, respectively, being lower as compared to standard SDT (IC50= 8.2 ± 1.9 μg/ml). Conclusion: Based on the results obtained it is appropriate to conclude that lichen are not only a good source of antioxidants, but also potent immunomodulators, and thus deserve to be investigated further.

Original languageEnglish
Pages (from-to)144-147
Number of pages4
JournalInternational Journal of Pharmacy and Pharmaceutical Sciences
Volume7
Issue number11
Publication statusPublished - 2015

Fingerprint

Lichens
Inhibitory Concentration 50
Ditiocarb
Respiratory Burst
Luminescence
Reactive Oxygen Species
Neutrophils
Luminol
Zymosan
Immunologic Factors
Phagocytes
Phagocytosis
Antioxidants
Macrophages
Serum

Keywords

  • Immunomodulatory activities
  • Lecanoric acid
  • Lichen compounds
  • Lobaric acid
  • Lumino-or lucigenin based chemiluminescence assay
  • Methyl haematomate
  • Methyl orsellinate
  • Orsellinic acid

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Immunomodulatory activities of some common lichen metabolites. / Thadhani, Vinitha M.; Mohammed Ahmed Hassan, Osman Mesaik; Asif, Muhammad; Karunaratne, Veranja; Choudhary, Iqbal M.

In: International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, No. 11, 2015, p. 144-147.

Research output: Contribution to journalArticle

Thadhani, Vinitha M. ; Mohammed Ahmed Hassan, Osman Mesaik ; Asif, Muhammad ; Karunaratne, Veranja ; Choudhary, Iqbal M. / Immunomodulatory activities of some common lichen metabolites. In: International Journal of Pharmacy and Pharmaceutical Sciences. 2015 ; Vol. 7, No. 11. pp. 144-147.
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