Abstract
CONTEXT: Cussonia arborea Hochst. ex A. Rich (Araliaceae) is a folk medicine used to treat various diseases. However, there is no report of the root phytochemistry.
OBJECTIVE: This study isolates and identifies the immunomodulatory compounds from root-bark of C. arborea.
MATERIALS AND METHODS: The methanol extract (18 g) was subjected to repeated column chromatography resulting in isolation of five compounds (1-5). Structure determination was achieved by analysis of their 1 D and 2 D NMR, and mass spectroscopy. The compounds (100-1.0 μg/mL) were examined immunomodulatory for effect on production of reactive oxygen species (ROS) from whole blood phagocytes and on proliferation of T-cells. The compounds cytotoxicity (100-1.0 μg/mL) was evaluated on NIH-3T3 normal fibroblast cells.
RESULTS: Three pentacyclic triterpenoids [3, 23-dihydroxy-12-oleanen-28-oic acid (1), 3β-hydroxylolean-12-en-28-oic (2) and 23-hydoxy-oxo-urs-12-en-28-oic acid (5)], two phytosterols: [stigmasterol (3)] and [3-O-β-d-glucopyranosyl stigmasterol (4)] were all isolated from the methanol soluble extract. All the tested compounds (1-4) were found to be nontoxic on NIH-3T3 cells. Compound 1 and 2 moderately inhibited the production of ROS (IC50 = 24.4 ± 4.3 and 37.5 ± 0.1 μg/mL, respectively) whereas compound 2 exhibited the highest inhibitory effect (IC50 = 12.6 ± 0.4 μg/mL) on proliferation of phytoheamagglutinin (PHA) activated T-cells.
CONCLUSIONS: The isolated compounds (1-5) are reported for the first time from this species. In addition, compound 2 with suppressive potential on production of intracellular ROS and proliferation of T-cells could be of immense value in control of autoimmune diseases as well as in immune compromised patients.
| Original language | English |
|---|---|
| Pages (from-to) | 2240-2247 |
| Number of pages | 8 |
| Journal | Pharmaceutical Biology |
| Volume | 55 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Dec 2017 |
| Externally published | Yes |
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Keywords
- Araliaceae
- hederagenin
- NMR spectroscopy
- oleanolic acid
- reactive oxygen species
- steroid
- stigmasterol
- T-cell proliferation assay
- triterpenoids
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology
- Pharmaceutical Science
- Drug Discovery
- Complementary and alternative medicine
Cite this
Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea. / Oladimeji, Abdulkabir Oladele; Oladosu, Ibrahim Adebayo; Jabeen, Almas; Faheem, Aisha; Mohammed Ahmed Hassan, Osman Mesaik; Ali, Muhammad Shaiq.
In: Pharmaceutical Biology, Vol. 55, No. 1, 01.12.2017, p. 2240-2247.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea
AU - Oladimeji, Abdulkabir Oladele
AU - Oladosu, Ibrahim Adebayo
AU - Jabeen, Almas
AU - Faheem, Aisha
AU - Mohammed Ahmed Hassan, Osman Mesaik
AU - Ali, Muhammad Shaiq
PY - 2017/12/1
Y1 - 2017/12/1
N2 - CONTEXT: Cussonia arborea Hochst. ex A. Rich (Araliaceae) is a folk medicine used to treat various diseases. However, there is no report of the root phytochemistry.OBJECTIVE: This study isolates and identifies the immunomodulatory compounds from root-bark of C. arborea.MATERIALS AND METHODS: The methanol extract (18 g) was subjected to repeated column chromatography resulting in isolation of five compounds (1-5). Structure determination was achieved by analysis of their 1 D and 2 D NMR, and mass spectroscopy. The compounds (100-1.0 μg/mL) were examined immunomodulatory for effect on production of reactive oxygen species (ROS) from whole blood phagocytes and on proliferation of T-cells. The compounds cytotoxicity (100-1.0 μg/mL) was evaluated on NIH-3T3 normal fibroblast cells.RESULTS: Three pentacyclic triterpenoids [3, 23-dihydroxy-12-oleanen-28-oic acid (1), 3β-hydroxylolean-12-en-28-oic (2) and 23-hydoxy-oxo-urs-12-en-28-oic acid (5)], two phytosterols: [stigmasterol (3)] and [3-O-β-d-glucopyranosyl stigmasterol (4)] were all isolated from the methanol soluble extract. All the tested compounds (1-4) were found to be nontoxic on NIH-3T3 cells. Compound 1 and 2 moderately inhibited the production of ROS (IC50 = 24.4 ± 4.3 and 37.5 ± 0.1 μg/mL, respectively) whereas compound 2 exhibited the highest inhibitory effect (IC50 = 12.6 ± 0.4 μg/mL) on proliferation of phytoheamagglutinin (PHA) activated T-cells.CONCLUSIONS: The isolated compounds (1-5) are reported for the first time from this species. In addition, compound 2 with suppressive potential on production of intracellular ROS and proliferation of T-cells could be of immense value in control of autoimmune diseases as well as in immune compromised patients.
AB - CONTEXT: Cussonia arborea Hochst. ex A. Rich (Araliaceae) is a folk medicine used to treat various diseases. However, there is no report of the root phytochemistry.OBJECTIVE: This study isolates and identifies the immunomodulatory compounds from root-bark of C. arborea.MATERIALS AND METHODS: The methanol extract (18 g) was subjected to repeated column chromatography resulting in isolation of five compounds (1-5). Structure determination was achieved by analysis of their 1 D and 2 D NMR, and mass spectroscopy. The compounds (100-1.0 μg/mL) were examined immunomodulatory for effect on production of reactive oxygen species (ROS) from whole blood phagocytes and on proliferation of T-cells. The compounds cytotoxicity (100-1.0 μg/mL) was evaluated on NIH-3T3 normal fibroblast cells.RESULTS: Three pentacyclic triterpenoids [3, 23-dihydroxy-12-oleanen-28-oic acid (1), 3β-hydroxylolean-12-en-28-oic (2) and 23-hydoxy-oxo-urs-12-en-28-oic acid (5)], two phytosterols: [stigmasterol (3)] and [3-O-β-d-glucopyranosyl stigmasterol (4)] were all isolated from the methanol soluble extract. All the tested compounds (1-4) were found to be nontoxic on NIH-3T3 cells. Compound 1 and 2 moderately inhibited the production of ROS (IC50 = 24.4 ± 4.3 and 37.5 ± 0.1 μg/mL, respectively) whereas compound 2 exhibited the highest inhibitory effect (IC50 = 12.6 ± 0.4 μg/mL) on proliferation of phytoheamagglutinin (PHA) activated T-cells.CONCLUSIONS: The isolated compounds (1-5) are reported for the first time from this species. In addition, compound 2 with suppressive potential on production of intracellular ROS and proliferation of T-cells could be of immense value in control of autoimmune diseases as well as in immune compromised patients.
KW - Araliaceae
KW - hederagenin
KW - NMR spectroscopy
KW - oleanolic acid
KW - reactive oxygen species
KW - steroid
KW - stigmasterol
KW - T-cell proliferation assay
KW - triterpenoids
UR - http://www.scopus.com/inward/record.url?scp=85049406582&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049406582&partnerID=8YFLogxK
U2 - 10.1080/13880209.2017.1400078
DO - 10.1080/13880209.2017.1400078
M3 - Article
C2 - 29141487
AN - SCOPUS:85049406582
VL - 55
SP - 2240
EP - 2247
JO - Pharmaceutical Biology
JF - Pharmaceutical Biology
SN - 1388-0209
IS - 1
ER -