Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea

Abdulkabir Oladele Oladimeji, Ibrahim Adebayo Oladosu, Almas Jabeen, Aisha Faheem, Osman Mesaik Mohammed Ahmed Hassan, Muhammad Shaiq Ali

Research output: Contribution to journalArticle

Abstract

CONTEXT: Cussonia arborea Hochst. ex A. Rich (Araliaceae) is a folk medicine used to treat various diseases. However, there is no report of the root phytochemistry.

OBJECTIVE: This study isolates and identifies the immunomodulatory compounds from root-bark of C. arborea.

MATERIALS AND METHODS: The methanol extract (18 g) was subjected to repeated column chromatography resulting in isolation of five compounds (1-5). Structure determination was achieved by analysis of their 1 D and 2 D NMR, and mass spectroscopy. The compounds (100-1.0 μg/mL) were examined immunomodulatory for effect on production of reactive oxygen species (ROS) from whole blood phagocytes and on proliferation of T-cells. The compounds cytotoxicity (100-1.0 μg/mL) was evaluated on NIH-3T3 normal fibroblast cells.

RESULTS: Three pentacyclic triterpenoids [3, 23-dihydroxy-12-oleanen-28-oic acid (1), 3β-hydroxylolean-12-en-28-oic (2) and 23-hydoxy-oxo-urs-12-en-28-oic acid (5)], two phytosterols: [stigmasterol (3)] and [3-O-β-d-glucopyranosyl stigmasterol (4)] were all isolated from the methanol soluble extract. All the tested compounds (1-4) were found to be nontoxic on NIH-3T3 cells. Compound 1 and 2 moderately inhibited the production of ROS (IC50 = 24.4 ± 4.3 and 37.5 ± 0.1 μg/mL, respectively) whereas compound 2 exhibited the highest inhibitory effect (IC50 = 12.6 ± 0.4 μg/mL) on proliferation of phytoheamagglutinin (PHA) activated T-cells.

CONCLUSIONS: The isolated compounds (1-5) are reported for the first time from this species. In addition, compound 2 with suppressive potential on production of intracellular ROS and proliferation of T-cells could be of immense value in control of autoimmune diseases as well as in immune compromised patients.

Original languageEnglish
Pages (from-to)2240-2247
Number of pages8
JournalPharmaceutical Biology
Volume55
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017
Externally publishedYes

Fingerprint

Araliaceae
Stigmasterol
Reactive Oxygen Species
T-Lymphocytes
Inhibitory Concentration 50
Methanol
Phytosterols
NIH 3T3 Cells
Acids
Traditional Medicine
Phagocytes
Autoimmune Diseases
Chromatography
Mass Spectrometry
Magnetic Resonance Spectroscopy
Fibroblasts

Keywords

  • Araliaceae
  • hederagenin
  • NMR spectroscopy
  • oleanolic acid
  • reactive oxygen species
  • steroid
  • stigmasterol
  • T-cell proliferation assay
  • triterpenoids

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea. / Oladimeji, Abdulkabir Oladele; Oladosu, Ibrahim Adebayo; Jabeen, Almas; Faheem, Aisha; Mohammed Ahmed Hassan, Osman Mesaik; Ali, Muhammad Shaiq.

In: Pharmaceutical Biology, Vol. 55, No. 1, 01.12.2017, p. 2240-2247.

Research output: Contribution to journalArticle

Oladimeji, Abdulkabir Oladele ; Oladosu, Ibrahim Adebayo ; Jabeen, Almas ; Faheem, Aisha ; Mohammed Ahmed Hassan, Osman Mesaik ; Ali, Muhammad Shaiq. / Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea. In: Pharmaceutical Biology. 2017 ; Vol. 55, No. 1. pp. 2240-2247.
@article{e1100758e3cc4caa9e34b17e643ea8c4,
title = "Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea",
abstract = "CONTEXT: Cussonia arborea Hochst. ex A. Rich (Araliaceae) is a folk medicine used to treat various diseases. However, there is no report of the root phytochemistry.OBJECTIVE: This study isolates and identifies the immunomodulatory compounds from root-bark of C. arborea.MATERIALS AND METHODS: The methanol extract (18 g) was subjected to repeated column chromatography resulting in isolation of five compounds (1-5). Structure determination was achieved by analysis of their 1 D and 2 D NMR, and mass spectroscopy. The compounds (100-1.0 μg/mL) were examined immunomodulatory for effect on production of reactive oxygen species (ROS) from whole blood phagocytes and on proliferation of T-cells. The compounds cytotoxicity (100-1.0 μg/mL) was evaluated on NIH-3T3 normal fibroblast cells.RESULTS: Three pentacyclic triterpenoids [3, 23-dihydroxy-12-oleanen-28-oic acid (1), 3β-hydroxylolean-12-en-28-oic (2) and 23-hydoxy-oxo-urs-12-en-28-oic acid (5)], two phytosterols: [stigmasterol (3)] and [3-O-β-d-glucopyranosyl stigmasterol (4)] were all isolated from the methanol soluble extract. All the tested compounds (1-4) were found to be nontoxic on NIH-3T3 cells. Compound 1 and 2 moderately inhibited the production of ROS (IC50 = 24.4 ± 4.3 and 37.5 ± 0.1 μg/mL, respectively) whereas compound 2 exhibited the highest inhibitory effect (IC50 = 12.6 ± 0.4 μg/mL) on proliferation of phytoheamagglutinin (PHA) activated T-cells.CONCLUSIONS: The isolated compounds (1-5) are reported for the first time from this species. In addition, compound 2 with suppressive potential on production of intracellular ROS and proliferation of T-cells could be of immense value in control of autoimmune diseases as well as in immune compromised patients.",
keywords = "Araliaceae, hederagenin, NMR spectroscopy, oleanolic acid, reactive oxygen species, steroid, stigmasterol, T-cell proliferation assay, triterpenoids",
author = "Oladimeji, {Abdulkabir Oladele} and Oladosu, {Ibrahim Adebayo} and Almas Jabeen and Aisha Faheem and {Mohammed Ahmed Hassan}, {Osman Mesaik} and Ali, {Muhammad Shaiq}",
year = "2017",
month = "12",
day = "1",
doi = "10.1080/13880209.2017.1400078",
language = "English",
volume = "55",
pages = "2240--2247",
journal = "Pharmaceutical Biology",
issn = "1388-0209",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea

AU - Oladimeji, Abdulkabir Oladele

AU - Oladosu, Ibrahim Adebayo

AU - Jabeen, Almas

AU - Faheem, Aisha

AU - Mohammed Ahmed Hassan, Osman Mesaik

AU - Ali, Muhammad Shaiq

PY - 2017/12/1

Y1 - 2017/12/1

N2 - CONTEXT: Cussonia arborea Hochst. ex A. Rich (Araliaceae) is a folk medicine used to treat various diseases. However, there is no report of the root phytochemistry.OBJECTIVE: This study isolates and identifies the immunomodulatory compounds from root-bark of C. arborea.MATERIALS AND METHODS: The methanol extract (18 g) was subjected to repeated column chromatography resulting in isolation of five compounds (1-5). Structure determination was achieved by analysis of their 1 D and 2 D NMR, and mass spectroscopy. The compounds (100-1.0 μg/mL) were examined immunomodulatory for effect on production of reactive oxygen species (ROS) from whole blood phagocytes and on proliferation of T-cells. The compounds cytotoxicity (100-1.0 μg/mL) was evaluated on NIH-3T3 normal fibroblast cells.RESULTS: Three pentacyclic triterpenoids [3, 23-dihydroxy-12-oleanen-28-oic acid (1), 3β-hydroxylolean-12-en-28-oic (2) and 23-hydoxy-oxo-urs-12-en-28-oic acid (5)], two phytosterols: [stigmasterol (3)] and [3-O-β-d-glucopyranosyl stigmasterol (4)] were all isolated from the methanol soluble extract. All the tested compounds (1-4) were found to be nontoxic on NIH-3T3 cells. Compound 1 and 2 moderately inhibited the production of ROS (IC50 = 24.4 ± 4.3 and 37.5 ± 0.1 μg/mL, respectively) whereas compound 2 exhibited the highest inhibitory effect (IC50 = 12.6 ± 0.4 μg/mL) on proliferation of phytoheamagglutinin (PHA) activated T-cells.CONCLUSIONS: The isolated compounds (1-5) are reported for the first time from this species. In addition, compound 2 with suppressive potential on production of intracellular ROS and proliferation of T-cells could be of immense value in control of autoimmune diseases as well as in immune compromised patients.

AB - CONTEXT: Cussonia arborea Hochst. ex A. Rich (Araliaceae) is a folk medicine used to treat various diseases. However, there is no report of the root phytochemistry.OBJECTIVE: This study isolates and identifies the immunomodulatory compounds from root-bark of C. arborea.MATERIALS AND METHODS: The methanol extract (18 g) was subjected to repeated column chromatography resulting in isolation of five compounds (1-5). Structure determination was achieved by analysis of their 1 D and 2 D NMR, and mass spectroscopy. The compounds (100-1.0 μg/mL) were examined immunomodulatory for effect on production of reactive oxygen species (ROS) from whole blood phagocytes and on proliferation of T-cells. The compounds cytotoxicity (100-1.0 μg/mL) was evaluated on NIH-3T3 normal fibroblast cells.RESULTS: Three pentacyclic triterpenoids [3, 23-dihydroxy-12-oleanen-28-oic acid (1), 3β-hydroxylolean-12-en-28-oic (2) and 23-hydoxy-oxo-urs-12-en-28-oic acid (5)], two phytosterols: [stigmasterol (3)] and [3-O-β-d-glucopyranosyl stigmasterol (4)] were all isolated from the methanol soluble extract. All the tested compounds (1-4) were found to be nontoxic on NIH-3T3 cells. Compound 1 and 2 moderately inhibited the production of ROS (IC50 = 24.4 ± 4.3 and 37.5 ± 0.1 μg/mL, respectively) whereas compound 2 exhibited the highest inhibitory effect (IC50 = 12.6 ± 0.4 μg/mL) on proliferation of phytoheamagglutinin (PHA) activated T-cells.CONCLUSIONS: The isolated compounds (1-5) are reported for the first time from this species. In addition, compound 2 with suppressive potential on production of intracellular ROS and proliferation of T-cells could be of immense value in control of autoimmune diseases as well as in immune compromised patients.

KW - Araliaceae

KW - hederagenin

KW - NMR spectroscopy

KW - oleanolic acid

KW - reactive oxygen species

KW - steroid

KW - stigmasterol

KW - T-cell proliferation assay

KW - triterpenoids

UR - http://www.scopus.com/inward/record.url?scp=85049406582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049406582&partnerID=8YFLogxK

U2 - 10.1080/13880209.2017.1400078

DO - 10.1080/13880209.2017.1400078

M3 - Article

VL - 55

SP - 2240

EP - 2247

JO - Pharmaceutical Biology

JF - Pharmaceutical Biology

SN - 1388-0209

IS - 1

ER -