Immunohistochemistry on rodent circulatory system

Its possible use in investigating hypertension

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Considering the increasingly strong footing of the role of vascular wall inflammation in hypertension, immunohistochemistry has become an important method to investigate the inflammatory responses in circulatory system. Immunohistochemistry is a method used to recognise the presence and location of constituents of tissues (as antigens) in situ by means of corresponding labelled antibodies. The principle of immunohistochemistry lies on the immunoreaction between antibodies to bind specifically to their corresponding antigens. Since it is done on a tissue rather than measuring inflammatory biomarker levels in serum, immunohistochemistry gives an overview of an intact tissue in the aspect of the localisation of a particular protein (i.e. in nucleus, cellular membrane or cytoplasm) and thus the expressions of inflammatory biomarkers may indicate what they are doing in vivo. In addition, the continuing advance in computer-aided image analysis has shifted immunohistochemistry from a traditional ‘merely descriptive’ technique to a quantitative measurement. Digital image evaluation enables measurements of protein activation by quantifying the colour density of positive immunostaining on a tissue. Therefore, immunohistochemistry is advantageous in providing not only qualitative but also semi-quantitative or quantitative information of protein expressions.

Original languageEnglish
Title of host publicationTechnical Aspects of Toxicological Immunohistochemistry: System Specific Biomarkers
PublisherSpringer New York
Pages147-177
Number of pages31
ISBN (Electronic)9781493915163
ISBN (Print)9781493915156
DOIs
Publication statusPublished - 1 Jan 2015

Fingerprint

circulatory system
Cardiovascular System
hypertension
immunohistochemistry
Rodentia
rodents
Immunohistochemistry
Hypertension
Tissue
Biomarkers
biomarkers
inflammation
antigens
Antigens
Proteins
antibodies
Antibodies
digital images
blood vessels
Image analysis

Keywords

  • Biological markers
  • Cardiovascular system
  • Hypertension
  • Inflammation
  • Rats

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ng, C. Y., Yusof, K., & Jaarin, K. (2015). Immunohistochemistry on rodent circulatory system: Its possible use in investigating hypertension. In Technical Aspects of Toxicological Immunohistochemistry: System Specific Biomarkers (pp. 147-177). Springer New York. https://doi.org/10.1007/978-1-4939-1516-3_8

Immunohistochemistry on rodent circulatory system : Its possible use in investigating hypertension. / Ng, Chun Yi; Yusof, Kamisah; Jaarin, Kamsiah.

Technical Aspects of Toxicological Immunohistochemistry: System Specific Biomarkers. Springer New York, 2015. p. 147-177.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ng, CY, Yusof, K & Jaarin, K 2015, Immunohistochemistry on rodent circulatory system: Its possible use in investigating hypertension. in Technical Aspects of Toxicological Immunohistochemistry: System Specific Biomarkers. Springer New York, pp. 147-177. https://doi.org/10.1007/978-1-4939-1516-3_8
Ng CY, Yusof K, Jaarin K. Immunohistochemistry on rodent circulatory system: Its possible use in investigating hypertension. In Technical Aspects of Toxicological Immunohistochemistry: System Specific Biomarkers. Springer New York. 2015. p. 147-177 https://doi.org/10.1007/978-1-4939-1516-3_8
Ng, Chun Yi ; Yusof, Kamisah ; Jaarin, Kamsiah. / Immunohistochemistry on rodent circulatory system : Its possible use in investigating hypertension. Technical Aspects of Toxicological Immunohistochemistry: System Specific Biomarkers. Springer New York, 2015. pp. 147-177
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