Immunohistochemical study of p16INK4A and survivin expressions in cervical squamous neoplasm

Geok Chin Tan, Sydee Norlatiffah, N. Akmal Sharifah, Ghazali Razmin, M. Sikik Shiran, A. Zailani Hatta, H. Oon Paul-Ng

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Introduction:Cervical cancer is the second most common cancer affecting Malaysian women. Despite the implementation of pap smear screening, many women are still diagnosed only in the advanced stage of cervical cancer. This could partly be due to failure of detection of its precursor lesions; hence the need to search for novel biomarkers to assist in the screening and diagnosis of cervical neoplasia. This study aims to determine the expression of p16 INK4A and survivin as possible predictive biomarkers in cervical squamous neoplasm. Material and Methods: This is a retrospective study on 201 cases of cervical neoplasm comprising of 129 cervical intraepithelial neoplasia (CIN) and 72 squamous cell carcinoma (SCC). All samples were evaluated by two independent observers using p16INK4A and survivin monoclonal antibodies. The p16INK4A expression was graded as negative, focal and diffuse positivity. The intensity for survivin expression was graded as weak, moderate and intense. Results: It is seen that p16INK4A expression in CIN 1, CIN 2 and CIN 3 were 25.4%, 42.9% and 95.9% respectively. Majority of SCC (98.6%) showed p16INK4A expression. Survivin expressions in CIN 1, CIN 2, CIN 3 and SCC were 56.7%, 33.4%, 87.5% and 98.6%. There was a linear relationship between increasing grade of CIN and p16INK4A expressions. Conclusion: Our study showed that p16INK4A expressions correlate well with the increasing grade of CIN. Although survivin does not correlate well to the increasing grade of CIN, it could be useful in differentiating CIN 3 from SCC.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalIndian Journal of Pathology and Microbiology
Volume53
Issue number1
DOIs
Publication statusPublished - 1 Jan 2010

Fingerprint

Cervical Intraepithelial Neoplasia
Uterine Cervical Neoplasms
Squamous Cell Carcinoma
Biomarkers
Cyclin-Dependent Kinase Inhibitor p16
Papanicolaou Test
Neoplasms
Retrospective Studies
Monoclonal Antibodies

Keywords

  • Cervical cancer
  • Cervix
  • Immunohistochemistry
  • P16
  • Survivin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Microbiology (medical)

Cite this

Immunohistochemical study of p16INK4A and survivin expressions in cervical squamous neoplasm. / Tan, Geok Chin; Norlatiffah, Sydee; Sharifah, N. Akmal; Razmin, Ghazali; Shiran, M. Sikik; Hatta, A. Zailani; Paul-Ng, H. Oon.

In: Indian Journal of Pathology and Microbiology, Vol. 53, No. 1, 01.01.2010, p. 1-6.

Research output: Contribution to journalArticle

Tan, Geok Chin ; Norlatiffah, Sydee ; Sharifah, N. Akmal ; Razmin, Ghazali ; Shiran, M. Sikik ; Hatta, A. Zailani ; Paul-Ng, H. Oon. / Immunohistochemical study of p16INK4A and survivin expressions in cervical squamous neoplasm. In: Indian Journal of Pathology and Microbiology. 2010 ; Vol. 53, No. 1. pp. 1-6.
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AU - Hatta, A. Zailani

AU - Paul-Ng, H. Oon

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AB - Introduction:Cervical cancer is the second most common cancer affecting Malaysian women. Despite the implementation of pap smear screening, many women are still diagnosed only in the advanced stage of cervical cancer. This could partly be due to failure of detection of its precursor lesions; hence the need to search for novel biomarkers to assist in the screening and diagnosis of cervical neoplasia. This study aims to determine the expression of p16 INK4A and survivin as possible predictive biomarkers in cervical squamous neoplasm. Material and Methods: This is a retrospective study on 201 cases of cervical neoplasm comprising of 129 cervical intraepithelial neoplasia (CIN) and 72 squamous cell carcinoma (SCC). All samples were evaluated by two independent observers using p16INK4A and survivin monoclonal antibodies. The p16INK4A expression was graded as negative, focal and diffuse positivity. The intensity for survivin expression was graded as weak, moderate and intense. Results: It is seen that p16INK4A expression in CIN 1, CIN 2 and CIN 3 were 25.4%, 42.9% and 95.9% respectively. Majority of SCC (98.6%) showed p16INK4A expression. Survivin expressions in CIN 1, CIN 2, CIN 3 and SCC were 56.7%, 33.4%, 87.5% and 98.6%. There was a linear relationship between increasing grade of CIN and p16INK4A expressions. Conclusion: Our study showed that p16INK4A expressions correlate well with the increasing grade of CIN. Although survivin does not correlate well to the increasing grade of CIN, it could be useful in differentiating CIN 3 from SCC.

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