Immunohistochemical Expression of π Class Glutathione S-Transferase and α-Fetoprotein in Hepatocellular Carcinoma and Chronic Liver Disease

Yasmin Anum Mohd Yusof, Khong Li Yan, Sharifah Noor Akmal Syed Hussain

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

OBJECTIVE: To determine whether tumor marker π glutathione transferase (GST-π) is expressed in hepatocellular carcinoma (HCC) and other chronic liver diseases and to compare its expression with that of α-fetoprotein (AFP). STUDY DESIGN: Samples used were formalin-fixed, paraffin-embedded liver tissues: normal (n = 3), chronic hepatitis B (n = 15), cirrhosis (n = 15) and HCC (n = 30). The expression of AFP and GST-π was detected by using immunohistochemistry with the peroxidase-antiperoxidase method. AFP immunoreactivity was based on the cytoplasm of the hepatocytes, while GST-π immunoreactivity was based on the nuclei of hepatocytes. RESULTS: In normal liver tissues, AFP was not expressed. However, there was strong staining of GST-π in bile duct epithelium cells and weak staining in hepatocytes. Our results showed higher AFP immunoreactivity in cases of HCC (36.7%) as compared to cirrhosis (6.7%) and hepatitis B (0%), whereas GST-π immunoreactivity was lower in cases of HCC (53.3%) as compared to cases of cirrhosis (100.0%) and hepatitis B (93.3%). Percent sensitivity of AFP determination for HCC was 36.7% as compared to 53.3% for GST-π, thus making GST-π a more senstive marker for detection of HCC. This study showed a significant relationship between the intensity and percentage of cells stained in hepatitis B, cirrhosis and HCC for GST-π immunoreactivity (P<.001, .001 and .05, respectively) but not for AFP (P<.05). Statistical analysis showed that there was no significant relationship between expression of AFP and GST-π in cirrhosis and HCC cases. Hepatitis B virus infection in HCC cases showed a positive rate of 46.7%, with AFP staining positively in 42.9% of tissues and GST-π staining positively in 57.1% of tissues. CONCLUSION: AFP is a diagnostic but rather insensitive tissue marker for HCC. However, the absence of AFP in benign chronic liver disease makes this marker useful in differentiating between HCC and other chronic liver diseases, whereas GST-π can be used as a diagnostic marker for HCC as well as in detecting other chronic liver diseases.

Original languageEnglish
Pages (from-to)332-338
Number of pages7
JournalAnalytical and Quantitative Cytology and Histology
Volume25
Issue number6
Publication statusPublished - Dec 2003

Fingerprint

Fetal Proteins
Glutathione Transferase
Liver Diseases
Hepatocellular Carcinoma
Chronic Disease
Fibrosis
Hepatitis B
Staining and Labeling
Hepatocytes
Liver
Chronic Hepatitis B
Virus Diseases
Tumor Biomarkers
Bile Ducts
Hepatitis B virus
Paraffin
Formaldehyde
Peroxidase

Keywords

  • Alpha-fetoproteins
  • Glutathione transferase
  • Hepatitis B
  • Hepatocellular carcinoma
  • Liver cirrhosis

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Cell Biology

Cite this

Immunohistochemical Expression of π Class Glutathione S-Transferase and α-Fetoprotein in Hepatocellular Carcinoma and Chronic Liver Disease. / Mohd Yusof, Yasmin Anum; Yan, Khong Li; Syed Hussain, Sharifah Noor Akmal.

In: Analytical and Quantitative Cytology and Histology, Vol. 25, No. 6, 12.2003, p. 332-338.

Research output: Contribution to journalArticle

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abstract = "OBJECTIVE: To determine whether tumor marker π glutathione transferase (GST-π) is expressed in hepatocellular carcinoma (HCC) and other chronic liver diseases and to compare its expression with that of α-fetoprotein (AFP). STUDY DESIGN: Samples used were formalin-fixed, paraffin-embedded liver tissues: normal (n = 3), chronic hepatitis B (n = 15), cirrhosis (n = 15) and HCC (n = 30). The expression of AFP and GST-π was detected by using immunohistochemistry with the peroxidase-antiperoxidase method. AFP immunoreactivity was based on the cytoplasm of the hepatocytes, while GST-π immunoreactivity was based on the nuclei of hepatocytes. RESULTS: In normal liver tissues, AFP was not expressed. However, there was strong staining of GST-π in bile duct epithelium cells and weak staining in hepatocytes. Our results showed higher AFP immunoreactivity in cases of HCC (36.7{\%}) as compared to cirrhosis (6.7{\%}) and hepatitis B (0{\%}), whereas GST-π immunoreactivity was lower in cases of HCC (53.3{\%}) as compared to cases of cirrhosis (100.0{\%}) and hepatitis B (93.3{\%}). Percent sensitivity of AFP determination for HCC was 36.7{\%} as compared to 53.3{\%} for GST-π, thus making GST-π a more senstive marker for detection of HCC. This study showed a significant relationship between the intensity and percentage of cells stained in hepatitis B, cirrhosis and HCC for GST-π immunoreactivity (P<.001, .001 and .05, respectively) but not for AFP (P<.05). Statistical analysis showed that there was no significant relationship between expression of AFP and GST-π in cirrhosis and HCC cases. Hepatitis B virus infection in HCC cases showed a positive rate of 46.7{\%}, with AFP staining positively in 42.9{\%} of tissues and GST-π staining positively in 57.1{\%} of tissues. CONCLUSION: AFP is a diagnostic but rather insensitive tissue marker for HCC. However, the absence of AFP in benign chronic liver disease makes this marker useful in differentiating between HCC and other chronic liver diseases, whereas GST-π can be used as a diagnostic marker for HCC as well as in detecting other chronic liver diseases.",
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T1 - Immunohistochemical Expression of π Class Glutathione S-Transferase and α-Fetoprotein in Hepatocellular Carcinoma and Chronic Liver Disease

AU - Mohd Yusof, Yasmin Anum

AU - Yan, Khong Li

AU - Syed Hussain, Sharifah Noor Akmal

PY - 2003/12

Y1 - 2003/12

N2 - OBJECTIVE: To determine whether tumor marker π glutathione transferase (GST-π) is expressed in hepatocellular carcinoma (HCC) and other chronic liver diseases and to compare its expression with that of α-fetoprotein (AFP). STUDY DESIGN: Samples used were formalin-fixed, paraffin-embedded liver tissues: normal (n = 3), chronic hepatitis B (n = 15), cirrhosis (n = 15) and HCC (n = 30). The expression of AFP and GST-π was detected by using immunohistochemistry with the peroxidase-antiperoxidase method. AFP immunoreactivity was based on the cytoplasm of the hepatocytes, while GST-π immunoreactivity was based on the nuclei of hepatocytes. RESULTS: In normal liver tissues, AFP was not expressed. However, there was strong staining of GST-π in bile duct epithelium cells and weak staining in hepatocytes. Our results showed higher AFP immunoreactivity in cases of HCC (36.7%) as compared to cirrhosis (6.7%) and hepatitis B (0%), whereas GST-π immunoreactivity was lower in cases of HCC (53.3%) as compared to cases of cirrhosis (100.0%) and hepatitis B (93.3%). Percent sensitivity of AFP determination for HCC was 36.7% as compared to 53.3% for GST-π, thus making GST-π a more senstive marker for detection of HCC. This study showed a significant relationship between the intensity and percentage of cells stained in hepatitis B, cirrhosis and HCC for GST-π immunoreactivity (P<.001, .001 and .05, respectively) but not for AFP (P<.05). Statistical analysis showed that there was no significant relationship between expression of AFP and GST-π in cirrhosis and HCC cases. Hepatitis B virus infection in HCC cases showed a positive rate of 46.7%, with AFP staining positively in 42.9% of tissues and GST-π staining positively in 57.1% of tissues. CONCLUSION: AFP is a diagnostic but rather insensitive tissue marker for HCC. However, the absence of AFP in benign chronic liver disease makes this marker useful in differentiating between HCC and other chronic liver diseases, whereas GST-π can be used as a diagnostic marker for HCC as well as in detecting other chronic liver diseases.

AB - OBJECTIVE: To determine whether tumor marker π glutathione transferase (GST-π) is expressed in hepatocellular carcinoma (HCC) and other chronic liver diseases and to compare its expression with that of α-fetoprotein (AFP). STUDY DESIGN: Samples used were formalin-fixed, paraffin-embedded liver tissues: normal (n = 3), chronic hepatitis B (n = 15), cirrhosis (n = 15) and HCC (n = 30). The expression of AFP and GST-π was detected by using immunohistochemistry with the peroxidase-antiperoxidase method. AFP immunoreactivity was based on the cytoplasm of the hepatocytes, while GST-π immunoreactivity was based on the nuclei of hepatocytes. RESULTS: In normal liver tissues, AFP was not expressed. However, there was strong staining of GST-π in bile duct epithelium cells and weak staining in hepatocytes. Our results showed higher AFP immunoreactivity in cases of HCC (36.7%) as compared to cirrhosis (6.7%) and hepatitis B (0%), whereas GST-π immunoreactivity was lower in cases of HCC (53.3%) as compared to cases of cirrhosis (100.0%) and hepatitis B (93.3%). Percent sensitivity of AFP determination for HCC was 36.7% as compared to 53.3% for GST-π, thus making GST-π a more senstive marker for detection of HCC. This study showed a significant relationship between the intensity and percentage of cells stained in hepatitis B, cirrhosis and HCC for GST-π immunoreactivity (P<.001, .001 and .05, respectively) but not for AFP (P<.05). Statistical analysis showed that there was no significant relationship between expression of AFP and GST-π in cirrhosis and HCC cases. Hepatitis B virus infection in HCC cases showed a positive rate of 46.7%, with AFP staining positively in 42.9% of tissues and GST-π staining positively in 57.1% of tissues. CONCLUSION: AFP is a diagnostic but rather insensitive tissue marker for HCC. However, the absence of AFP in benign chronic liver disease makes this marker useful in differentiating between HCC and other chronic liver diseases, whereas GST-π can be used as a diagnostic marker for HCC as well as in detecting other chronic liver diseases.

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KW - Hepatocellular carcinoma

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