Immunohistochemical detection of phospho-akt, phospho-bad, her2 and oestrogen receptors α and β in malaysian breast cancer patients

Heng Fong Seow, Wai Kien Yip, Hui Woon Loh, Hairuszah Ithnin, Patricia Por, Rohaizak Muhammad

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Activation of Akt signaling pathway has been documented in various human malignancies, including breast carcinoma. The objective of this study is to determine the incidence of Akt phosphorylation in breast tumours and its relationship with expression of ER-α, ER-β, HER2, Ki-67 and phosphorylated Bcl-2 associated death domain (p-BAD). Immunohistochemical staining was performed to detect these molecules on 43 paraffin-embedded breast tumour tissues with commercially available antibodies. Eighteen (41.9%), 3 (7.0%), 23 (53.5%), 35 (81.4%), 21 (48.8%), 29 (67.4%), and 34 (81.0%) of breast tumours were positive for nuclear ER-α, nuclear ER-β, membranous HER2, cytonuclear p-Akt (Thr308), p-Akt (Ser473), p-BAD and Ki-67, respectively. ER-α expression was inversely correlated with HER2 and Ki-67 (P=0.041 and P=0.040, respectively). The p-Akt (Ser473) was correlated with increased level of p-BAD (Ser136) (P=0.012). No relationship of Akt phosphorylation with HER2, ER-α or ER-β was found. The p-Akt (Ser473) immunoreactivity was significantly higher in stage IV than in stage I or II (P=0.036 or P=0.009). The higher Ki-67 and lower ER-α expression showed an association with patient age of <50 years (P=0.004) and with positive nodal status(P=0.033), respectively. Our data suggest that the Aktphosphorylation and inactivation of its downstream target, BAD may play a role in survival of breast cancer cell. This study does not support the simple model of linear HER2/ PI3K/Akt pathway in breast cancer.

Original languageEnglish
Pages (from-to)239-248
Number of pages10
JournalPathology and Oncology Research
Volume16
Issue number2
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Estrogen Receptors
Breast Neoplasms
Phosphorylation
Phosphatidylinositol 3-Kinases
Paraffin
Linear Models
Staining and Labeling
Survival
Antibodies
Incidence
Death Domain
Neoplasms

Keywords

  • Akt
  • Breast cancer
  • HER2
  • Oestrogen receptor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Immunohistochemical detection of phospho-akt, phospho-bad, her2 and oestrogen receptors α and β in malaysian breast cancer patients. / Seow, Heng Fong; Yip, Wai Kien; Loh, Hui Woon; Ithnin, Hairuszah; Por, Patricia; Muhammad, Rohaizak.

In: Pathology and Oncology Research, Vol. 16, No. 2, 06.2010, p. 239-248.

Research output: Contribution to journalArticle

Seow, Heng Fong ; Yip, Wai Kien ; Loh, Hui Woon ; Ithnin, Hairuszah ; Por, Patricia ; Muhammad, Rohaizak. / Immunohistochemical detection of phospho-akt, phospho-bad, her2 and oestrogen receptors α and β in malaysian breast cancer patients. In: Pathology and Oncology Research. 2010 ; Vol. 16, No. 2. pp. 239-248.
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