Immunogenic eimeria tenella glycosylphosphatidylinositol-anchored surface antigens (SAGs) induce inflammatory responses in avian macrophages

Chow Yock Ping, Kiew Lian Wan, Damer P. Blake, Fiona Tomley, Sheila Nathan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: At least 19 glycosylphosphatidylinositol (GPI)-anchored surface antigens (SAGs) are expressed specifically by second-generation merozoites of Eimeria tenella, but the ability of these proteins to stimulate immune responses in the chicken is unknown. Methodology/Principal Findings: Ten SAGs, belonging to two previously defined multigene families (A and B), were expressed as soluble recombinant (r) fusion proteins in E. coli. Chicken macrophages were treated with purified rSAGs and changes in macrophage nitrite production, and in mRNA expression profiles of inducible nitric oxide synthase (iNOS) and of a panel of cytokines were measured. Treatment with rSAGs 4, 5, and 12 induced high levels of macrophage nitric oxide production and IL-1β mRNA transcription that may contribute to the inflammatory response observed during E. tenella infection. Concomitantly, treatment with rSAGs 4, 5 and 12 suppressed the expression of IL-12 and IFN-γ and elevated that of IL-10, suggesting that during infection these molecules may specifically impair the development of cellular mediated immunity. Conclusions/Significance: In summary, some E. tenella SAGs appear to differentially modulate chicken innate and humoral immune responses and those derived from multigene family A (especially rSAG 12) may be more strongly linked with E. tenella pathogenicity associated with the endogenous second generation stages.

Original languageEnglish
Article numbere25233
JournalPLoS One
Volume6
Issue number9
DOIs
Publication statusPublished - 28 Sep 2011

Fingerprint

Eimeria tenella
Glycosylphosphatidylinositols
Macrophages
surface antigens
Surface Antigens
macrophages
inflammation
Chickens
Multigene Family
chickens
multigene family
Messenger RNA
Nitric Oxide Synthase Type II
recombinant fusion proteins
Transcription
Interleukin-12
Recombinant Fusion Proteins
Nitrites
Interleukin-1
Merozoites

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Immunogenic eimeria tenella glycosylphosphatidylinositol-anchored surface antigens (SAGs) induce inflammatory responses in avian macrophages. / Yock Ping, Chow; Wan, Kiew Lian; Blake, Damer P.; Tomley, Fiona; Nathan, Sheila.

In: PLoS One, Vol. 6, No. 9, e25233, 28.09.2011.

Research output: Contribution to journalArticle

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