Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss

S. M. Rahim, Mazlan Abd. Ghaffar, Simon Kumar Das, J. P. Delaunoy, P. Laurent

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Pseudobranch function has long interested scientists, but its role has yet to be elucidated. Several studies have suggested that pseudobranchs serve respiratory, osmoregulatory, and sensory functions. This work investigated the immunolocalization of pseudobranch carbonic anhydrase (CA) in the teleost fish species rainbow trout (Oncorhynchus mykiss) to clarify its physiological function. CA was purified from rainbow trout gills O. mykiss and specific antibodies were raised. Immunoblotting between tissue homogenates of pseudobranch and gill CA antibodies showed specific immunostaining with only one band corresponding to CA in the pseudobranch homogenate. Results of immunohistochemical technique revealed that CA was distributed within pseudobranch cells and more precisely in the apical parts (anti-vascular) of cells. The basal (vascular) parts of cells, tubular system, blood capillaries, and pillar cells were not immunostained. Immunocytochemistry confirmed these results and showed that some CA enzyme was cytoplasmic and the remainder was linked to membranous structures. The results also showed that the lacunar tissue layers did not display immunoperoxidase activity. Our results indicated that pseudobranch CA may have a function related to the extracellular medium wherein CA intervenes with the mechanism of stimulation of afferent nerve fibers.

Original languageEnglish
Pages (from-to)194-200
Number of pages7
JournalJournal of Zhejiang University: Science B
Volume15
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Carbonic Anhydrases
Oncorhynchus mykiss
carbonate dehydratase
Tissue
blood vessels
Blood Vessels
gills
cells
blood capillaries
antibodies
tissues
Antibodies
immunocytochemistry
nerve fibers
immunoblotting
Nerve Fibers
Immunoblotting
Fish
Fishes
Blood

Keywords

  • Carbonic anhydrase
  • Immunocytochemistry
  • Immunohistochemistry
  • Physiology
  • Pseudobranch
  • Rainbow trout

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • veterinary(all)

Cite this

Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss. / Rahim, S. M.; Abd. Ghaffar, Mazlan; Das, Simon Kumar; Delaunoy, J. P.; Laurent, P.

In: Journal of Zhejiang University: Science B, Vol. 15, No. 2, 2014, p. 194-200.

Research output: Contribution to journalArticle

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