Immuno strip test for detection of pork adulteration in cooked meatballs

Bambang Kuswandi, Agus Abdul Gani, Musa Ahmad

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A rapid immuno strip test was developed to provide visual detection of pork adulteration in processed meats. Gold nanoparticles was prepared and conjugated with anti-Swine IgG polyclonal antibody. A lateral flow immunosensor was developed, and then were used to test pork adulterated in processed meats, i.e. beef meatball samples. The simulated samples consisted of pork-in-beef meatball at the ratio of 0–100 (%w/w) adulteration levels that were extracted in phosphate-buffered solution. Pure beef meatball without pork was included as controls. The response time was completed in 5 min, after incubation time. Detection limit of the immunosensor strip was 0.1% (w/w). This immunosensor tests can be applied to detect low levels of pork adulteration in beef meatballs. The reliability of the assay was further investigated by comparing the results to those of commercially available ELISA kits. It was obtained that the correlation of two methods was excellent. Thus, the strip could provide a simple approach to detect pork adulteration in processed meats samples with high reliability.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalFood Bioscience
Volume19
DOIs
Publication statusPublished - 1 Sep 2017
Externally publishedYes

Fingerprint

Beef
adulterated products
Immunosensors
pork
Meats
beef
testing
Meat
Gold
Assays
Immunoglobulin G
Phosphates
Nanoparticles
polyclonal antibodies
sampling
Red Meat
Antibodies
detection limit
enzyme-linked immunosorbent assay
phosphates

Keywords

  • Beef meatball
  • Immunosensor
  • Pork adulteration
  • Processed meats
  • Strip test

ASJC Scopus subject areas

  • Food Science
  • Biochemistry

Cite this

Immuno strip test for detection of pork adulteration in cooked meatballs. / Kuswandi, Bambang; Gani, Agus Abdul; Ahmad, Musa.

In: Food Bioscience, Vol. 19, 01.09.2017, p. 1-6.

Research output: Contribution to journalArticle

Kuswandi, Bambang ; Gani, Agus Abdul ; Ahmad, Musa. / Immuno strip test for detection of pork adulteration in cooked meatballs. In: Food Bioscience. 2017 ; Vol. 19. pp. 1-6.
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