Immunization with the recombinant Burkholderia pseudomallei outer membrane protein Omp85 induces protective immunity in mice

Yu Ching Su, Kiew Lian Wan, Rahmah Mohamed, Sheila Nathan

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Burkholderia pseudomallei is resistant to a wide range of antibiotics, leading to relapse and recrudescence of melioidosis after cessation of antibiotic therapy. More effective immunotherapies are needed for better management of melioidosis. We evaluated the prophylactic potential of the immunogenic outer membrane protein Omp85 as a vaccine against murine melioidosis. Immunization of BALB/c mice with recombinant Omp85 (rOmp85) triggered a Th2-type immune response. Up to 70% of the immunized animals were protected against infectious challenge of B. pseudomallei with reduced bacterial load in extrapulmonary organs. Mouse anti-rOmp85 promoted complement-mediated killing and opsonophagocytosis of B. pseudomallei by human polymorphonuclear cells. In conclusion, we demonstrated that B. pseudomallei Omp85 is potentially able to induce protective immunity against melioidosis.

Original languageEnglish
Pages (from-to)5005-5011
Number of pages7
JournalVaccine
Volume28
Issue number31
DOIs
Publication statusPublished - Jul 2010

Fingerprint

Burkholderia pseudomallei
Melioidosis
outer membrane proteins
Immunity
Immunization
immunization
Membrane Proteins
immunity
mice
antibiotics
Anti-Bacterial Agents
Recurrence
Bacterial Load
immunotherapy
relapse
Immunotherapy
complement
Vaccines
immune response
vaccines

Keywords

  • Bacteriocidal
  • Immunotherapy
  • Melioidosis
  • Opsonophagocytosis

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Immunization with the recombinant Burkholderia pseudomallei outer membrane protein Omp85 induces protective immunity in mice. / Su, Yu Ching; Wan, Kiew Lian; Mohamed, Rahmah; Nathan, Sheila.

In: Vaccine, Vol. 28, No. 31, 07.2010, p. 5005-5011.

Research output: Contribution to journalArticle

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